Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VAB027_RS15390 Genome accession   NZ_CP010812
Coordinates   3323533..3324759 (+) Length   408 a.a.
NCBI ID   WP_046127052.1    Uniprot ID   -
Organism   Vibrio cholerae strain 10432-62     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3318533..3329759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAB027_RS15370 (VAB027_3047) ampD 3319333..3319878 (-) 546 WP_046127049.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VAB027_RS15375 (VAB027_3048) nadC 3320174..3321064 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VAB027_RS15380 (VAB027_3049) - 3321309..3321779 (+) 471 WP_046127050.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VAB027_RS15385 (VAB027_3050) pilB 3321802..3323490 (+) 1689 WP_046127051.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VAB027_RS15390 (VAB027_3051) pilC 3323533..3324759 (+) 1227 WP_046127052.1 type II secretion system F family protein Machinery gene
  VAB027_RS15395 (VAB027_3052) pilD 3324817..3325692 (+) 876 WP_046127053.1 A24 family peptidase Machinery gene
  VAB027_RS15400 (VAB027_3053) coaE 3325689..3326297 (+) 609 WP_046127054.1 dephospho-CoA kinase -
  VAB027_RS15405 (VAB027_3054) zapD 3326329..3327069 (+) 741 WP_000207194.1 cell division protein ZapD -
  VAB027_RS15410 (VAB027_3055) yacG 3327227..3327424 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  VAB027_RS15415 (VAB027_3056) parC 3327473..3329758 (-) 2286 WP_046127055.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45134.43 Da        Isoelectric Point: 10.3424

>NTDB_id=140001 VAB027_RS15390 WP_046127052.1 3323533..3324759(+) (pilC) [Vibrio cholerae strain 10432-62]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPLLASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=140001 VAB027_RS15390 WP_046127052.1 3323533..3324759(+) (pilC) [Vibrio cholerae strain 10432-62]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTATCTCTTTTGGCACGCCTAACCCATCGCGTGAAAAGTAAAGATATTACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCCATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCACTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGATTTAGTGGAAACCGGAGAGATGTCCGGCAACTTACCTGAAGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTGGTGTTGGTCGCGCTCGGGGTATC
TTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACCC
AGCAAGTGCTGAAACTCTCACACTGGGTACAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCTTTCCAGATCCGTTTAAAAACCAGCCGCTTGGGGCTGAAATTTCCGATTATTGGCAA
TGTACTCGCCAAAGCGTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCGCTCCTCGCCA
GTTTAAAAACCACGGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGAAGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGGCAATTAGATGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTGTTTCTTGGGACGGTCGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.755

100

0.998
  pilC Vibrio campbellii strain DS40M4

73.5

98.039

0.721
  pilC Acinetobacter baumannii D1279779

42.892

100

0.429
  pilC Acinetobacter baylyi ADP1

42.26

99.755

0.422
  pilC Legionella pneumophila strain ERS1305867

42.462

97.549

0.414
  pilC Pseudomonas stutzeri DSM 10701

42.424

97.059

0.412
  pilG Neisseria gonorrhoeae MS11

41.542

98.529

0.409
  pilG Neisseria meningitidis 44/76-A

41.045

98.529

0.404

Multiple sequence alignment