Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VAB027_RS15385 Genome accession   NZ_CP010812
Coordinates   3321802..3323490 (+) Length   562 a.a.
NCBI ID   WP_046127051.1    Uniprot ID   -
Organism   Vibrio cholerae strain 10432-62     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3316802..3328490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAB027_RS15360 (VAB027_3045) fldB 3317131..3317649 (+) 519 WP_000690112.1 flavodoxin FldB -
  VAB027_RS15365 (VAB027_3046) - 3317696..3319075 (-) 1380 WP_046127048.1 IS4-like element ISVa18 family transposase -
  VAB027_RS15370 (VAB027_3047) ampD 3319333..3319878 (-) 546 WP_046127049.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VAB027_RS15375 (VAB027_3048) nadC 3320174..3321064 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VAB027_RS15380 (VAB027_3049) - 3321309..3321779 (+) 471 WP_046127050.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VAB027_RS15385 (VAB027_3050) pilB 3321802..3323490 (+) 1689 WP_046127051.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VAB027_RS15390 (VAB027_3051) pilC 3323533..3324759 (+) 1227 WP_046127052.1 type II secretion system F family protein Machinery gene
  VAB027_RS15395 (VAB027_3052) pilD 3324817..3325692 (+) 876 WP_046127053.1 A24 family peptidase Machinery gene
  VAB027_RS15400 (VAB027_3053) coaE 3325689..3326297 (+) 609 WP_046127054.1 dephospho-CoA kinase -
  VAB027_RS15405 (VAB027_3054) zapD 3326329..3327069 (+) 741 WP_000207194.1 cell division protein ZapD -
  VAB027_RS15410 (VAB027_3055) yacG 3327227..3327424 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62136.26 Da        Isoelectric Point: 6.0411

>NTDB_id=140000 VAB027_RS15385 WP_046127051.1 3321802..3323490(+) (pilB) [Vibrio cholerae strain 10432-62]
MLTNLVAILRQAELISVTQEQAVVEHISASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=140000 VAB027_RS15385 WP_046127051.1 3321802..3323490(+) (pilB) [Vibrio cholerae strain 10432-62]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGTGACGCAAGAACAAGCTGTGGTCGAACACAT
CAGCGCCTCGGGCACTTCCGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAGGAACTCACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGTTACGACTACGCCCCCCTGTGCCAACAGCTTGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTCGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCGCTAGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCGATTCAAGGCGCCGCCAATCAGGGTAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATCTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGTCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCACAAGATGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGTGTATCCACACTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTGATGACAGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTGTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCCGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCACACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACACTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCATTTCAGCCCAATGAA
ATTTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAGCGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACATTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGTTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

98.577

100

0.986
  pilB Vibrio parahaemolyticus RIMD 2210633

73.665

100

0.737
  pilB Vibrio campbellii strain DS40M4

73.488

100

0.735
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50.192

92.527

0.464
  pilF Neisseria gonorrhoeae MS11

45.907

100

0.459
  pilF Thermus thermophilus HB27

39.372

96.263

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

38

97.865

0.372
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37

Multiple sequence alignment