Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AS52_RS16185 Genome accession   NZ_CP010586
Coordinates   3234397..3234768 (+) Length   123 a.a.
NCBI ID   WP_372454473.1    Uniprot ID   -
Organism   Priestia megaterium Q3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3229397..3239768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS52_RS16160 (AS52_03341) - 3229698..3230531 (-) 834 WP_049165544.1 AraC family transcriptional regulator -
  AS52_RS16165 (AS52_03342) - 3230734..3231555 (+) 822 WP_049165546.1 sensor histidine kinase -
  AS52_RS16170 (AS52_03343) - 3231556..3232197 (+) 642 WP_033580507.1 response regulator transcription factor -
  AS52_RS16175 (AS52_03344) - 3232332..3233711 (+) 1380 WP_049165551.1 PepSY domain-containing protein -
  AS52_RS16180 (AS52_03345) - 3233741..3234205 (+) 465 WP_049165553.1 FixH family protein -
  AS52_RS16185 (AS52_03346) nucA/comI 3234397..3234768 (+) 372 WP_372454473.1 DNA-entry nuclease Machinery gene
  AS52_RS16190 (AS52_03347) - 3234923..3235276 (+) 354 WP_223272376.1 DUF1516 family protein -
  AS52_RS16195 (AS52_03348) - 3235316..3235909 (-) 594 WP_028414498.1 hypothetical protein -
  AS52_RS16200 (AS52_03349) - 3236062..3236718 (-) 657 WP_047751820.1 TetR/AcrR family transcriptional regulator -
  AS52_RS16205 (AS52_03350) - 3236741..3238018 (-) 1278 WP_049166379.1 glycosyltransferase -
  AS52_RS16210 (AS52_03351) - 3238351..3238836 (-) 486 WP_049165556.1 hypothetical protein -
  AS52_RS16215 (AS52_03352) fsa 3238915..3239583 (-) 669 WP_028414502.1 fructose-6-phosphate aldolase -

Sequence

Protein


Download         Length: 123 a.a.        Molecular weight: 13533.79 Da        Isoelectric Point: 4.3246

>NTDB_id=139435 AS52_RS16185 WP_372454473.1 3234397..3234768(+) (nucA/comI) [Priestia megaterium Q3]
MIEGNPLEQNSSTNPSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLKGIPTKSGYDRDEFPMAF
CEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 372 bp        

>NTDB_id=139435 AS52_RS16185 WP_372454473.1 3234397..3234768(+) (nucA/comI) [Priestia megaterium Q3]
ATGATTGAAGGAAATCCGTTAGAGCAGAACTCTTCAACGAATCCCTCCTACGATGAAGTCATTACCTTCCCTGCTGATCG
TTACCCTGAAACTGCAGCGCACATTCAACATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGATCGAGACGGTG
CTGAAGAAAACCGCCGTGAGTCTTTAAAAGGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTT
TGTGAAGAAGGCGGAAGCGGCGCAGATATTGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGGTCTTGGATTTCACA
TCAAGTAGATGAGTTTCCAGACGGTACGAAGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(26-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.4

100

0.593

Multiple sequence alignment