Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EG09_RS25655 Genome accession   NZ_CP010555
Coordinates   5586300..5587517 (-) Length   405 a.a.
NCBI ID   WP_015648061.1    Uniprot ID   A0A9P1W172
Organism   Pseudomonas aeruginosa strain FRD1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5581300..5592517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG09_RS25610 (EG09_25610) - 5581485..5581934 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  EG09_RS25615 (EG09_25615) - 5581931..5582559 (-) 629 Protein_5142 DUF1780 domain-containing protein -
  EG09_RS25620 (EG09_25620) - 5582692..5583117 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  EG09_RS25625 (EG09_25625) - 5583114..5583584 (+) 471 WP_003103866.1 hypothetical protein -
  EG09_RS25630 (EG09_25630) - 5583581..5584270 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  EG09_RS25635 (EG09_25635) - 5584376..5584585 (+) 210 WP_003094660.1 hypothetical protein -
  EG09_RS25640 (EG09_25640) yacG 5584622..5584822 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  EG09_RS25645 (EG09_25645) coaE 5584819..5585430 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  EG09_RS25650 (EG09_25650) pilD 5585427..5586299 (-) 873 WP_015648060.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EG09_RS25655 (EG09_25655) pilC 5586300..5587517 (-) 1218 WP_015648061.1 type II secretion system F family protein Machinery gene
  EG09_RS25660 (EG09_25660) pilB 5587521..5589221 (-) 1701 WP_030048379.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EG09_RS25665 (EG09_25665) - 5589452..5589916 (+) 465 WP_023129290.1 pilin -
  EG09_RS25670 (EG09_25670) - 5590074..5591387 (+) 1314 WP_015648064.1 O-antigen ligase family protein -
  EG09_RS25680 (EG09_25680) nadC 5591568..5592416 (-) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44024.90 Da        Isoelectric Point: 9.8103

>NTDB_id=139220 EG09_RS25655 WP_015648061.1 5586300..5587517(-) (pilC) [Pseudomonas aeruginosa strain FRD1]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTHIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=139220 EG09_RS25655 WP_015648061.1 5586300..5587517(-) (pilC) [Pseudomonas aeruginosa strain FRD1]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGG
TCATCAACATTTCCAACGTACTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCCACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAGTCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

60.784

100

0.612
  pilC Acinetobacter baumannii D1279779

60.149

99.753

0.6
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria gonorrhoeae MS11

46.04

99.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Vibrio campbellii strain DS40M4

40.898

99.012

0.405
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment