Detailed information NTG0136630

Bioinformatically predicted

Overview

• Name: comM/pilM
• Type: Machinery gene
• Locus tag: AO353_RS12475
• Genome accession: NZ_CP012830
• Coordinates: 2745782..2746846 (+)
• Length: 354 a.a.
• NCBI ID: WP_054595227.1
• Uniprot ID: -
• Organism: Pseudomonas fluorescens strain FW300-N2E3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2740782..2751846

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AO353_RS12465 (AO353_12465)	-	2742665..2743054 (+)	390	WP_054593867.1	DUF6124 family protein	-
AO353_RS12470 (AO353_12470)	-	2743127..2745565 (-)	2439	WP_371917632.1	penicillin-binding protein 1A	-
AO353_RS12475 (AO353_12475)	comM/pilM	2745782..2746846 (+)	1065	WP_054595227.1	pilus assembly protein PilM	Machinery gene
AO353_RS12480 (AO353_12480)	-	2746846..2747412 (+)	567	WP_054595228.1	PilN domain-containing protein	-
AO353_RS12485 (AO353_12485)	pilO	2747409..2748032 (+)	624	WP_054595229.1	type 4a pilus biogenesis protein PilO	Machinery gene
AO353_RS12490 (AO353_12490)	pilP	2748029..2748556 (+)	528	WP_054595230.1	pilus assembly protein PilP	Machinery gene
AO353_RS12495 (AO353_12495)	pilQ	2748570..2750651 (+)	2082	WP_054595231.1	type IV pilus secretin PilQ	Machinery gene
AO353_RS12500 (AO353_12500)	aroK	2750656..2751174 (+)	519	WP_054595232.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38145.76 Da
• Isoelectric Point: 4.8232

>NTDB_id=136630 AO353_RS12475 WP_054595227.1 2745782..2746846(+) (comM/pilM) [Pseudomonas fluorescens strain FW300-N2E3]
MLGLFNKKSRTLLGIDISSTSVKLLELSRAGNRYRVEAYAVEPLPTNAVVEKNIAELEGVGQALSRVLLKAKTSVKSVAV
AVAGSAVITKIIEMDAGLSPDEMENQLKIEADQYIPYPLEEVAIDFEVQGASRRNPERVDVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLTAQLAGVTERSTVAVVDIGATMTTLSVLSNGRIIYTREQLFGGRQLTEEIQRRYGMTV
EQAGLAKKQGGLPEDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAIDYILLAGGTASVTGLDRLIEQRLGTPTQVANP
FADMTLSSKVNASALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=136630 AO353_RS12475 WP_054595227.1 2745782..2746846(+) (comM/pilM) [Pseudomonas fluorescens strain FW300-N2E3]
GTGCTGGGACTCTTCAATAAAAAAAGCCGCACGCTTTTGGGGATCGATATCAGCTCCACTTCGGTGAAGCTTCTCGAGCT
AAGCCGTGCAGGCAATCGATACCGGGTCGAAGCCTACGCGGTAGAGCCGCTGCCGACCAACGCCGTGGTCGAGAAAAATA
TCGCCGAACTCGAAGGTGTCGGGCAGGCGTTGTCCCGGGTGTTGCTCAAGGCCAAAACCAGCGTCAAGAGTGTCGCGGTG
GCGGTTGCCGGATCGGCGGTCATCACCAAGATCATCGAAATGGACGCCGGGCTTTCCCCCGATGAAATGGAAAATCAGCT
GAAGATTGAAGCTGACCAGTACATCCCCTATCCCCTCGAAGAAGTCGCCATCGATTTTGAAGTGCAGGGCGCCTCCCGGC
GTAATCCCGAGCGGGTCGACGTATTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAAGTTCGCGAAGCCGCGCTGGCCCTT
GCCGGCCTGACCGCACGGGTCGTCGATGTCGAGGCGTACGCGCTGGAGCGTTCATTCGGCTTGCTGACGGCACAGTTGGC
CGGCGTGACCGAGCGCTCGACGGTAGCCGTGGTCGACATCGGCGCCACCATGACCACCTTGAGCGTGCTGTCCAACGGCC
GAATCATCTACACCCGCGAGCAGTTGTTCGGCGGTCGTCAGTTGACGGAGGAAATCCAGCGCCGCTATGGCATGACGGTT
GAACAGGCCGGTCTTGCAAAAAAACAGGGTGGCTTGCCTGAAGACTACGTCAGCGAAGTCCTGCAGCCGTTCCGCGAGGC
GCTGGTGCAGCAGGTTTCGCGATCGTTGCAGTTCTTTTTCGCCTCAGGTCAGTACAACGCGATCGACTACATCCTGCTGG
CCGGTGGCACAGCCTCGGTCACCGGGCTGGATCGACTGATCGAGCAGCGCCTGGGCACGCCGACGCAGGTGGCCAATCCG
TTTGCGGACATGACGTTGAGCAGCAAGGTCAACGCCAGTGCCCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGCGG
CCTGGCGCTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM/pilM	Acinetobacter baylyi ADP1	55.493	100	0.556
pilM	Acinetobacter baumannii D1279779	54.52	100	0.545
comM	Acinetobacter nosocomialis M2	54.52	100	0.545
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466