Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RR32_RS16425 Genome accession   NZ_CP010368
Coordinates   3499664..3501376 (+) Length   570 a.a.
NCBI ID   WP_004710829.1    Uniprot ID   K9BAJ6
Organism   Acinetobacter nosocomialis strain 6411     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3494664..3506376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR32_RS16390 (RR32_16395) nusA 3495554..3497038 (-) 1485 WP_002156952.1 transcription termination factor NusA -
  RR32_RS16395 (RR32_16400) rimP 3497076..3497600 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  RR32_RS16415 (RR32_16420) secG 3498237..3498566 (-) 330 WP_002156913.1 preprotein translocase subunit SecG -
  RR32_RS16420 (RR32_16425) tpiA 3498579..3499373 (-) 795 WP_032047906.1 triose-phosphate isomerase -
  RR32_RS16425 (RR32_16430) pilB 3499664..3501376 (+) 1713 WP_004710829.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RR32_RS16430 (RR32_16435) pilC 3501404..3502630 (+) 1227 WP_039249002.1 type II secretion system F family protein Machinery gene
  RR32_RS20340 (RR32_16440) pilD 3502630..3503490 (+) 861 WP_032047902.1 prepilin peptidase Machinery gene
  RR32_RS16440 (RR32_16445) coaE 3503492..3504088 (+) 597 WP_032047901.1 dephospho-CoA kinase -
  RR32_RS16445 (RR32_16450) - 3504085..3504999 (-) 915 WP_031955876.1 DMT family transporter -
  RR32_RS16450 (RR32_16455) rlmB 3505035..3505784 (-) 750 WP_004883555.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  RR32_RS16455 (RR32_16460) - 3505886..3506212 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63457.85 Da        Isoelectric Point: 5.2413

>NTDB_id=136630 RR32_RS16425 WP_004710829.1 3499664..3501376(+) (pilB) [Acinetobacter nosocomialis strain 6411]
MSVLHTSPKFTGFFRRLVEEKHVSAATMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=136630 RR32_RS16425 WP_004710829.1 3499664..3501376(+) (pilB) [Acinetobacter nosocomialis strain 6411]
ATGTCAGTATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCCAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTACATCTCTCCC
CTTCTTTATTGGCTGAAACCATTTCTGCGGAGTTTGCTGAACCATATTTTGATCTAGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGACAAATTCTATATGT
CGCGACCAGCAACCCAAGCAATATTGAAGCAATCGATGCAATCCGTTTTAACAGTAAATTGCTGGTTGAACCTGTTATTG
TCGAGCATCATAAGCTGGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATGGTCCTACCACTCAAGAAGATGAAGAAGAAACACCTCAAGGTGATGAAGCTCCTATTGT
TAAATATATTAATAAGTTGCTTATTGATGCCATTCGTATGGGCGCGTCAGATTTACATTTTGAGCCTTATGAAAAGTCAT
ATCGGGTACGTTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCGCCTTTACAACTTGCAAACCGTTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGTCGTATCAAACTTAAACTATCAAA
GACCAAAGCAATTGATTTCCGTGTGAACTCGCTCCCTACCTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGCATTGATGCATTAGGCTACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTGATTACTGGACCAACAGGCTCGGGTAAAACTGTATCTTTATATACAGGCTTAAATATTCTAAATAC
TGAAAGTTCCAATATTTCTACTGCCGAAGATCCAGTGGAAATTAACTTAGAAGGTATTAATCAGGTTAACGTCAATCCTA
AAGTGGGTTTAACGTTTGCAGCTGCTCTTAAGTCTTTTTTACGTCAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCTGCTCAAACCGGCCACATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTCTACGTAATATGGGGGTTCCTTCTTTTAATATTGCAACATCGGTAAATCTAGTGATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGTAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACATCCAGATTTTCGGGTTTTTCAACCCGTGGGCTGTCCTGAATGTCGTGAAGGTTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9BAJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

98.596

100

0.986
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365

Multiple sequence alignment