Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FORC9_RS01265 Genome accession   NZ_CP009984
Coordinates   257695..259479 (+) Length   594 a.a.
NCBI ID   WP_011079356.1    Uniprot ID   A0A3Q0L3M5
Organism   Vibrio vulnificus strain FORC_009     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 252695..264479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC9_RS01245 (FORC9_0225) pilM 255009..256016 (+) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  FORC9_RS01250 (FORC9_0226) pilN 256006..256596 (+) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  FORC9_RS01255 (FORC9_0227) pilO 256589..257179 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC9_RS01260 (FORC9_0228) pilP 257169..257681 (+) 513 WP_060530656.1 pilus assembly protein PilP Machinery gene
  FORC9_RS01265 (FORC9_0229) pilQ 257695..259479 (+) 1785 WP_011079356.1 type IV pilus secretin PilQ Machinery gene
  FORC9_RS01270 (FORC9_0230) aroK 259681..260199 (+) 519 WP_011079357.1 shikimate kinase AroK -
  FORC9_RS01275 (FORC9_0231) aroB 260232..261323 (+) 1092 WP_017789764.1 3-dehydroquinate synthase -
  FORC9_RS01280 (FORC9_0232) - 261374..262870 (+) 1497 WP_060530657.1 AAA family ATPase -
  FORC9_RS01285 (FORC9_0233) - 262950..263777 (+) 828 WP_039554836.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64520.62 Da        Isoelectric Point: 4.5316

>NTDB_id=133451 FORC9_RS01265 WP_011079356.1 257695..259479(+) (pilQ) [Vibrio vulnificus strain FORC_009]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGSEGGSASIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=133451 FORC9_RS01265 WP_011079356.1 257695..259479(+) (pilQ) [Vibrio vulnificus strain FORC_009]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACCTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAACGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACGGTGGTGGATTTGAAACCGGTAGCGGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTCAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTCTCGACCGTGGTTGAAAG
TATCGAAGTGTTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTTAGTGGTGAATTTCGTCACGACTATCGTCTGT
CTGGACGCTATCTCGAGGTGACAATTTCTCGTATCAAATCGGAACAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGTTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCCGTGACTGGTAATGTGACGTTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGATATCA
TTCTTCAAGTGAAAGGTTTAGATAAACGTGTTGAAGGTAATGTTGTGCTCATCGCTCCTCAAGATGAATTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAACGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATCATCAAAGTCAACTTTGC
CAAAGCGTCAGACATCGCTGAGATGATCAATGGCAAAGAGTCGGTTAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTTAAACAGGTACAAATTGAAGCGCGAATTGTCACGGTGAACGAAGGCAATCTTGATGAGCTTGGTGTACGTTG
GGGTTTCACCTCCATCAACGGCAGCACAACTGTCGGTGGTTCAATCGAAAACAACCTGGCAACCATTGGTCTCTACAATG
CAGGCAGTGGTAGTGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGTAGTGAAGGCGGTTCGGCCTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACCTCTGCGAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAACTCTCTGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACGGAGATCCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCATTT
AAGAAAGCCGTGCTTAGCCTGAAAGTGACCCCACAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCCGGCGAAATTGTCAAAACAGGCACTGGAGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGCATTACCAATACCACGGACAAGGTTCCTCTG
TTAGGCGATTTACCTTTATTGGGGGCGCTTTTCCGCCGTAGCTATGAAAACGTGGGTAAACGTGAATTGCTGATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3M5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.966

99.327

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

73.729

99.327

0.732
  pilQ Vibrio cholerae strain A1552

73.729

99.327

0.732
  pilQ Legionella pneumophila strain ERS1305867

41.156

96.128

0.396
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

39.539

94.949

0.375
  comQ Acinetobacter baylyi ADP1

37.162

99.663

0.37

Multiple sequence alignment