Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC9_RS01245 Genome accession   NZ_CP009984
Coordinates   255009..256016 (+) Length   335 a.a.
NCBI ID   WP_011151279.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_009     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 250009..261016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC9_RS01230 (FORC9_0222) - 250840..251112 (-) 273 WP_060530654.1 DUF3624 domain-containing protein -
  FORC9_RS01235 (FORC9_0223) oxyR 251386..252285 (+) 900 WP_039554843.1 DNA-binding transcriptional regulator OxyR -
  FORC9_RS01240 (FORC9_0224) - 252356..254869 (-) 2514 WP_060530655.1 penicillin-binding protein 1A -
  FORC9_RS01245 (FORC9_0225) pilM 255009..256016 (+) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  FORC9_RS01250 (FORC9_0226) pilN 256006..256596 (+) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  FORC9_RS01255 (FORC9_0227) pilO 256589..257179 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC9_RS01260 (FORC9_0228) pilP 257169..257681 (+) 513 WP_060530656.1 pilus assembly protein PilP Machinery gene
  FORC9_RS01265 (FORC9_0229) pilQ 257695..259479 (+) 1785 WP_011079356.1 type IV pilus secretin PilQ Machinery gene
  FORC9_RS01270 (FORC9_0230) aroK 259681..260199 (+) 519 WP_011079357.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37638.05 Da        Isoelectric Point: 6.3873

>NTDB_id=133447 FORC9_RS01245 WP_011151279.1 255009..256016(+) (pilM) [Vibrio vulnificus strain FORC_009]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVALAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=133447 FORC9_RS01245 WP_011151279.1 255009..256016(+) (pilM) [Vibrio vulnificus strain FORC_009]
ATGGGTAAGCCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAACTGCCTCTTTTCCGTTATCAGGTGGCACTGGCGGTGCCTGATAGTTCA
GTAATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAGCGAGAATTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGTGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCTGTGCTG
GTAGATGTGCATGCGCATAGTCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGATAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCGCCTTTAGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAACTGACAGAGCGAGTTCGTCGGCAATTGCAGCTGTTCCAATCTATGGATAGCCAAAGCAAAGTGAATGGCCTCTGGAT
CTGCGGTGAAGGCGCGATTACTCCGTTGTTGAGTGAAACGTTGCAGAGAAAGCTGAGTTTGGAGTGCGAAGTCCTTAATC
CTCTGGCTCTGTTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTGGCAGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.433

100

0.534
  pilM Vibrio cholerae strain A1552

49.267

100

0.501

Multiple sequence alignment