Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FORC8_RS02880 Genome accession   NZ_CP009982
Coordinates   583173..583577 (-) Length   134 a.a.
NCBI ID   WP_015297250.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_008     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 578173..588577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC8_RS02860 (FORC8_0485) coaE 578692..579306 (-) 615 WP_025633754.1 dephospho-CoA kinase -
  FORC8_RS02865 (FORC8_0486) pilD 579307..580176 (-) 870 WP_005493974.1 prepilin peptidase Machinery gene
  FORC8_RS02870 (FORC8_0487) pilC 580241..581464 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  FORC8_RS02875 (FORC8_0488) pilB 581488..583173 (-) 1686 WP_020835398.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC8_RS02880 (FORC8_0489) pilA 583173..583577 (-) 405 WP_015297250.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FORC8_RS02885 (FORC8_0490) nadC 583841..584728 (-) 888 WP_025442237.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC8_RS02890 (FORC8_0491) ampD 584821..585372 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC8_RS02895 (FORC8_0492) pdhR 585778..586545 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13659.55 Da        Isoelectric Point: 6.4433

>NTDB_id=133418 FORC8_RS02880 WP_015297250.1 583173..583577(-) (pilA) [Vibrio parahaemolyticus strain FORC_008]
MKHSKQKKQQGFTLIELMIVVAIIGVLSAIAVPAYQNYVAKSEAATALGSLRALVTPAELKLQQDGELSGVVADLGGSAS
HALGAITTSGANISAATLTFTFNTGSLSGDAITLTKTSSGWTCTDGTTVLDNCN

Nucleotide


Download         Length: 405 bp        

>NTDB_id=133418 FORC8_RS02880 WP_015297250.1 583173..583577(-) (pilA) [Vibrio parahaemolyticus strain FORC_008]
ATGAAACACAGTAAACAGAAAAAACAACAGGGTTTTACCTTGATTGAATTGATGATTGTGGTGGCGATTATTGGGGTGTT
GTCAGCAATTGCAGTACCTGCTTATCAAAATTATGTCGCTAAAAGTGAAGCGGCAACGGCATTAGGAAGTTTGAGAGCAT
TAGTCACTCCTGCTGAACTTAAACTTCAACAAGATGGAGAGCTATCTGGTGTAGTAGCTGACTTAGGTGGATCTGCATCA
CATGCATTAGGTGCTATTACAACATCTGGTGCTAACATAAGCGCTGCTACTCTAACCTTCACGTTTAATACAGGCTCATT
AAGTGGTGATGCTATCACACTAACCAAAACAAGTTCTGGTTGGACATGTACTGATGGCACTACGGTTTTAGACAACTGTA
ACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-130)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

52.143

100

0.545
  pilA Pseudomonas aeruginosa PAK

42.446

100

0.44
  pilA Vibrio cholerae strain A1552

42.029

100

0.433
  pilA Vibrio cholerae C6706

42.029

100

0.433
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.029

100

0.433
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.188

95.522

0.403
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.535

96.269

0.381

Multiple sequence alignment