Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC6_RS02935 Genome accession   NZ_CP009765
Coordinates   594211..595896 (-) Length   561 a.a.
NCBI ID   WP_025613047.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 589211..600896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC6_RS02905 (FORC6_0488) rplS 589601..589954 (+) 354 WP_005462554.1 50S ribosomal protein L19 -
  FORC6_RS02910 (FORC6_0489) yacG 590383..590577 (-) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  FORC6_RS02915 (FORC6_0490) zapD 590646..591386 (-) 741 WP_023624252.1 cell division protein ZapD -
  FORC6_RS02920 (FORC6_0491) coaE 591414..592028 (-) 615 WP_060657297.1 dephospho-CoA kinase -
  FORC6_RS02925 (FORC6_0492) pilD 592029..592898 (-) 870 WP_015297252.1 prepilin peptidase Machinery gene
  FORC6_RS02930 (FORC6_0493) pilC 592963..594186 (-) 1224 WP_025505035.1 type II secretion system F family protein Machinery gene
  FORC6_RS02935 (FORC6_0494) pilB 594211..595896 (-) 1686 WP_025613047.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC6_RS02940 (FORC6_0495) - 595896..596354 (-) 459 WP_025613046.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC6_RS02945 (FORC6_0496) nadC 596618..597505 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC6_RS02950 (FORC6_0497) ampD 597598..598149 (+) 552 WP_060657299.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC6_RS02955 (FORC6_0498) pdhR 598555..599322 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62289.21 Da        Isoelectric Point: 5.4031

>NTDB_id=131999 FORC6_RS02935 WP_025613047.1 594211..595896(-) (pilB) [Vibrio parahaemolyticus strain FORC_006]
MLTNLSTILRQNGLLTFPQEESLIEQVKASGISMPEALLSSGFFTSSELAAHLSSIFGLSQPELSQYEYAALCQQLGLRE
LITRHNALPLHRTSSTLLLAIADPTNLQAEDDFRFATGLQVELVLVDFRELSAAIRRLYGRAIGQERSQLKEINQEELAS
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKIYRVRLRCDGILIETQQPPNHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETIIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCKQPQEHTVQLQHLGIQTTDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDESLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=131999 FORC6_RS02935 WP_025613047.1 594211..595896(-) (pilB) [Vibrio parahaemolyticus strain FORC_006]
ATGCTAACCAACCTCTCAACAATTCTTCGCCAAAACGGGTTACTGACTTTTCCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATCTCGATGCCGGAAGCTTTACTCAGTTCTGGATTCTTCACGTCAAGCGAACTCGCCGCACACTTAA
GTTCTATTTTTGGCTTAAGTCAGCCCGAGTTATCTCAATATGAGTATGCTGCGCTTTGCCAACAGCTCGGCCTCAGAGAG
TTAATCACACGACATAATGCACTCCCGCTCCACCGTACTTCTTCAACTTTATTATTAGCGATTGCCGACCCCACGAATCT
ACAAGCCGAAGATGACTTCCGCTTTGCCACTGGTTTGCAGGTCGAACTGGTTCTGGTCGATTTTCGCGAACTAAGTGCCG
CAATTCGTCGCTTGTACGGGCGTGCCATTGGTCAGGAACGCTCCCAGCTCAAAGAGATCAACCAGGAAGAGCTAGCCAGC
TTAGTGGATGTCGGTGCAGATGAAATCGACAACATCGAAGACTTGAGCCAGGACGAGTCGCCCGTCAGCCGCTACATCAA
CCAAATTTTACTGGATGCGGTACGTAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATATACCGAGTTCGTC
TACGTTGCGATGGCATTTTGATCGAAACTCAGCAACCGCCAAATCACTTGAGTCGTCGCTTATCAGCTCGTATCAAAATT
CTCTCTAAACTTGATATTGCCGAGCGACGTTTACCACAAGATGGGCGAATTAAACTCAAACTGAATCAAGACACCGCGAT
TGATATGCGGGTATCCACGCTACCTACCTTGTTTGGAGAGAAAATCGTACTCCGACTGCTCGATAGCAGCTCTGCATCAC
TGGATATCGACAAGCTTGGTTATAGTGAGCAACAAAAACAGCTGTATTTAGAGGCTCTGCGTCGTCCACAAGGTATGATT
CTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCGTTGTACACTGGGTTAAATATTCTCAATAAACCAGAGATCAA
CATCTCCACAGCGGAAGACCCGGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAACCTAAGATTGGTTTTG
GCTTTGCAGAAGCTCTACGTTCTTTTCTGCGTCAAGATCCCGATGTGGTCATGGTGGGTGAGATTCGAGACCTAGATACT
GCGGAAATCGCGATTAAGGCGTCCCAAACCGGTCACTTAGTGCTCTCTACTTTGCATACCAACTCTGCGGCTGAAACCAT
CATTCGTTTATCAAACATGGGCGTAGAAAGTTTTAACCTCGCCTCTTCTCTTAGTTTGATTATTGCCCAACGCCTAGCTC
GCAAACTTTGTCCATATTGTAAACAACCGCAAGAGCATACCGTTCAACTTCAGCACCTCGGCATTCAAACAACTGACAAC
ATCTTTAAAGCCAATCCAGATGGTTGCAACGAATGCACCCATGGTTATTCTGGCCGAACGGGTATCTATGAAGTCATGCG
CTTTGATGAATCTCTATCCGAAGCTCTTATTAAAGGAGCCTCGGTACATGAACTGGAAAAGCTTGCCATTGCGAACGGCA
TGAGCACCTTGCAAATGTCCGGAATAGAAAAACTCAAACAAGGCATCACAAGCTTTAGTGAGTTACAACGCGTGCTCTAT
TTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

96.613

100

0.966
  pilB Vibrio campbellii strain DS40M4

89.84

100

0.898
  pilB Vibrio cholerae strain A1552

74.021

100

0.742
  pilB Acinetobacter baumannii D1279779

51.316

94.831

0.487
  pilB Legionella pneumophila strain ERS1305867

49.165

96.078

0.472
  pilB Acinetobacter baylyi ADP1

50.87

92.157

0.469
  pilF Neisseria gonorrhoeae MS11

45.213

100

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

37.669

100

0.398
  pilF Thermus thermophilus HB27

37.102

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.155

100

0.365

Multiple sequence alignment