Detailed information NTG0131089

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NG74_RS12050
• Genome accession: NZ_CP009679
• Coordinates: 2540764..2541378 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain JS25R
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2535764..2546378

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NG74_RS12025 (NG74_02491)	rpsT	2536067..2536333 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
NG74_RS12030 (NG74_02492)	holA	2536350..2537391 (-)	1042	Protein_2382	DNA polymerase III subunit delta	-
NG74_RS20145	-	2537431..2537582 (-)	152	Protein_2383	hypothetical protein	-
NG74_RS19750	-	2537623..2537757 (+)	135	WP_003152870.1	YqzM family protein	-
NG74_RS12040 (NG74_02493)	comEC	2537776..2540127 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NG74_RS12045 (NG74_02494)	-	2540128..2540697 (-)	570	WP_003152868.1	ComE operon protein 2	-
NG74_RS12050 (NG74_02495)	comEA	2540764..2541378 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
NG74_RS12055 (NG74_02496)	comER	2541437..2542258 (+)	822	WP_014418420.1	late competence protein ComER	-
NG74_RS12060 (NG74_02497)	-	2542327..2543064 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
NG74_RS12065 (NG74_02498)	rsfS	2543061..2543417 (-)	357	WP_007408260.1	ribosome silencing factor	-
NG74_RS12070 (NG74_02499)	yqeK	2543435..2543995 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NG74_RS12075 (NG74_02500)	-	2543985..2544554 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
NG74_RS12080 (NG74_02501)	yhbY	2544565..2544855 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
NG74_RS12085 (NG74_02502)	aroE	2544849..2545691 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=131089 NG74_RS12050 WP_014418419.1 2540764..2541378(-) (comEA) [Bacillus velezensis strain JS25R]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=131089 NG74_RS12050 WP_014418419.1 2540764..2541378(-) (comEA) [Bacillus velezensis strain JS25R]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363