Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JT17_RS05915 Genome accession   NZ_CP009237
Coordinates   1223057..1224442 (-) Length   461 a.a.
NCBI ID   WP_044009244.1    Uniprot ID   -
Organism   Glaesserella parasuis strain KL0318     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1218057..1229442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JT17_RS05890 (JT17_06095) clpP 1218074..1218655 (+) 582 WP_044009240.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  JT17_RS05895 (JT17_06100) clpX 1218662..1219912 (+) 1251 WP_044009241.1 ATP-dependent protease ATP-binding subunit ClpX -
  JT17_RS05900 (JT17_06105) dapE 1219986..1221119 (-) 1134 WP_044009242.1 succinyl-diaminopimelate desuccinylase -
  JT17_RS05905 (JT17_06110) - 1221203..1221871 (-) 669 WP_044009243.1 prepilin peptidase -
  JT17_RS05910 (JT17_06115) pilC 1221868..1223064 (-) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  JT17_RS05915 (JT17_06120) pilB 1223057..1224442 (-) 1386 WP_044009244.1 GspE/PulE family protein Machinery gene
  JT17_RS05920 (JT17_06125) pilA 1224490..1224954 (-) 465 WP_005711781.1 pilin Machinery gene
  JT17_RS05925 (JT17_06130) radA 1225142..1226518 (+) 1377 WP_021110380.1 DNA repair protein RadA -
  JT17_RS05930 (JT17_06135) gmk 1226571..1227197 (-) 627 WP_035491622.1 guanylate kinase -
  JT17_RS05935 (JT17_06140) - 1227266..1228237 (-) 972 WP_044009245.1 IS110 family transposase -
  JT17_RS05940 (JT17_06145) gpt 1228638..1229117 (-) 480 WP_005714020.1 xanthine phosphoribosyltransferase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52699.53 Da        Isoelectric Point: 5.2524

>NTDB_id=127478 JT17_RS05915 WP_044009244.1 1223057..1224442(-) (pilB) [Glaesserella parasuis strain KL0318]
MSQYTICEQQTERIFDISPSRWQKNCEEKQLLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPLYEESELAFAEQEQEQLNLSDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGVGC
DECYQGYKGRIGVYQCLSRTAKKFDKQTAYLDFSCLRESAKQKIEQQLTNETEVDRVLGYE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=127478 JT17_RS05915 WP_044009244.1 1223057..1224442(-) (pilB) [Glaesserella parasuis strain KL0318]
ATGTCCCAATACACTATCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAAAAAAATTGCGA
GGAGAAACAGCTTTTATTGCGTTACCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCTGTGGTGATTGCTTCTGATGAA
CTTAAATACTTGCTTAACGCACTCTCGCCTGAGCAACAACCCCTTTATGAAGAGAGCGAACTGGCTTTCGCAGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAGCCCAAAATGCGT
CCGACATTCATATTGAACCACGCAAGCAAAAATTGATTATTCGCCTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCCTTTACAGCGCCTTAAACTATCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCCGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTGTTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTCTCTGCGGTCGAACGCTTGTTGCAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTGATTGCACAACGCCTTGTGCGTAAGCTCTGTAAAAAGTGTCGTGGAGTAGGCTGT
GACGAATGCTATCAAGGCTATAAAGGGCGGATCGGGGTTTACCAATGTTTAAGCAGAACGGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGATTTTTCCTGCCTACGAGAGAGTGCTAAACAGAAAATTGAACAACAACTCACCAATGAAACCGAAG
TGGATAGGGTATTAGGTTATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

98.482

100

0.985
  pilB Haemophilus influenzae 86-028NP

57.174

99.783

0.57
  pilB Haemophilus influenzae Rd KW20

57.802

98.698

0.57
  pilB Vibrio cholerae strain A1552

38.659

100

0.425
  pilB Vibrio campbellii strain DS40M4

39.337

100

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

39.13

100

0.41
  pilB Acinetobacter baumannii D1279779

36.381

100

0.406
  pilB Acinetobacter baylyi ADP1

36.832

100

0.403
  pilB Legionella pneumophila strain ERS1305867

40.909

95.445

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.58

100

0.384
  pilF Neisseria gonorrhoeae MS11

37.288

100

0.382

Multiple sequence alignment