Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NCDO2118_RS11630 Genome accession   NZ_CP009054
Coordinates   2368959..2370032 (-) Length   357 a.a.
NCBI ID   WP_012898623.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis NCDO 2118     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2363959..2375032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCDO2118_RS11585 (NCDO2118_2221) - 2364098..2364907 (-) 810 WP_012898616.1 metal ABC transporter permease -
  NCDO2118_RS11590 (NCDO2118_2222) - 2364900..2365637 (-) 738 WP_012898617.1 metal ABC transporter ATP-binding protein -
  NCDO2118_RS11595 (NCDO2118_2223) - 2365814..2366656 (-) 843 WP_012898618.1 metal ABC transporter substrate-binding protein -
  NCDO2118_RS11600 (NCDO2118_2224) - 2366653..2367090 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  NCDO2118_RS11605 (NCDO2118_2225) comGG 2367171..2367455 (-) 285 WP_012898619.1 competence type IV pilus minor pilin ComGG Machinery gene
  NCDO2118_RS11610 (NCDO2118_2226) comGF 2367494..2367940 (-) 447 WP_038603761.1 competence type IV pilus minor pilin ComGF Machinery gene
  NCDO2118_RS11615 (NCDO2118_2227) comGE 2367903..2368199 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  NCDO2118_RS11620 (NCDO2118_2228) comGD 2368171..2368602 (-) 432 WP_012898621.1 competence type IV pilus minor pilin ComGD Machinery gene
  NCDO2118_RS11625 (NCDO2118_2229) comGC 2368562..2368945 (-) 384 WP_012898622.1 competence type IV pilus major pilin ComGC Machinery gene
  NCDO2118_RS11630 (NCDO2118_2230) comGB 2368959..2370032 (-) 1074 WP_012898623.1 competence type IV pilus assembly protein ComGB Machinery gene
  NCDO2118_RS11635 (NCDO2118_2231) comGA 2369926..2370864 (-) 939 WP_012898624.1 competence type IV pilus ATPase ComGA Machinery gene
  NCDO2118_RS11640 - 2371134..2371316 (-) 183 WP_038603763.1 hypothetical protein -
  NCDO2118_RS11645 (NCDO2118_2232) - 2371307..2372062 (-) 756 WP_012898625.1 hypothetical protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40392.54 Da        Isoelectric Point: 10.3132

>NTDB_id=126453 NCDO2118_RS11630 WP_012898623.1 2368959..2370032(-) (comGB) [Lactococcus lactis subsp. lactis NCDO 2118]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTAFSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=126453 NCDO2118_RS11630 WP_012898623.1 2368959..2370032(-) (comGB) [Lactococcus lactis subsp. lactis NCDO 2118]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAGAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTAAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTCTCAGTAACTCTTTTTAAACGAAAATCGGCAATAATGAATTTTACATTTCTTGTTAAATTACCTT
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAGGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAACTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

82.991

95.518

0.793
  comYB Streptococcus gordonii str. Challis substr. CH1

53.666

95.518

0.513
  comYB Streptococcus mutans UA140

53.079

95.518

0.507
  comYB Streptococcus mutans UA159

52.786

95.518

0.504
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae R6

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae D39

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis SK321

50.746

93.838

0.476
  comGB/cglB Streptococcus mitis NCTC 12261

49.851

93.838

0.468

Multiple sequence alignment