Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HZ99_RS19745 Genome accession   NZ_CP008896
Coordinates   4413823..4415154 (+) Length   443 a.a.
NCBI ID   WP_038445429.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain UK4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4408823..4420154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ99_RS19725 (HZ99_20160) pilM 4411420..4412073 (+) 654 WP_038445425.1 type IV pilus biogenesis protein PilM -
  HZ99_RS19730 (HZ99_20165) - 4412073..4412624 (+) 552 WP_038445427.1 PilN domain-containing protein -
  HZ99_RS19735 (HZ99_20170) - 4412621..4413241 (+) 621 WP_051903194.1 type 4a pilus biogenesis protein PilO -
  HZ99_RS19740 (HZ99_20175) - 4413370..4413753 (+) 384 Protein_3974 pilus assembly protein PilP -
  HZ99_RS19745 (HZ99_20180) pilQ 4413823..4415154 (+) 1332 WP_038445429.1 type IV pilus secretin PilQ Machinery gene
  HZ99_RS19750 (HZ99_20185) aroK 4415159..4415677 (+) 519 WP_038445430.1 shikimate kinase AroK -
  HZ99_RS19755 (HZ99_20190) aroB 4415825..4416925 (+) 1101 WP_038445432.1 3-dehydroquinate synthase -
  HZ99_RS19760 (HZ99_20195) - 4416936..4418510 (+) 1575 WP_038445434.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 48050.62 Da        Isoelectric Point: 4.6408

>NTDB_id=125532 HZ99_RS19745 WP_038445429.1 4413823..4415154(+) (pilQ) [Pseudomonas fluorescens strain UK4]
MTEAVPKRIDLIQLPPPGASPLGNGRGSFDGDRLSLNFQDIEVRAVLQQIADVAGLNLVAGDDVQGSITLRLKDVPWDQA
LDLVLQAKGLDQRVTANVLLVAPAEELAARERLALESRKLMTELAPLRRELLQVNYARAAELAKLFQSVGGSEGVADERG
SVTVDDRTNNIIAYQTGERLEELRRIVNQLDVPVRQVMIEARIVEANVDYDKSVGVRWGGLLRNKGNWSGGGVDNSPSGE
GESPDSSPFVDLGVVNASSGLGIAFVTDNVLLDLELTAMEKGGNGEVISQPKVVTSDKETARILKGTEIPYQEATSEGAT
SVSFKEASLSLEVTPQITPDGQVIMEVRVTKDEPDYLNKLNDVPPIKKNEVNAKVQVKDGETIVLGGVFSKNQSKVVDKV
PFLGDVPYLGRLFRRDVVAQRKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=125532 HZ99_RS19745 WP_038445429.1 4413823..4415154(+) (pilQ) [Pseudomonas fluorescens strain UK4]
ATGACTGAGGCTGTGCCCAAGAGAATCGACCTGATTCAGTTGCCACCTCCCGGTGCTTCGCCTCTGGGCAATGGGCGTGG
CTCCTTTGACGGTGACAGGTTGTCCCTCAATTTCCAGGACATCGAGGTCCGCGCAGTACTGCAGCAGATTGCCGATGTGG
CCGGCCTCAACCTGGTGGCCGGCGATGATGTGCAGGGCTCGATTACCTTGCGCCTCAAGGATGTGCCCTGGGATCAAGCC
CTGGATCTGGTGCTGCAGGCCAAGGGCCTCGATCAACGCGTGACGGCCAATGTGCTGCTGGTGGCGCCGGCCGAGGAACT
GGCCGCGCGCGAGCGGCTGGCCCTGGAATCACGCAAGTTGATGACCGAACTGGCGCCGCTGCGGCGGGAGCTGTTGCAAG
TCAATTACGCCAGGGCGGCCGAGTTGGCCAAACTGTTCCAGTCGGTGGGCGGGTCTGAGGGGGTGGCTGATGAGCGTGGT
TCGGTGACGGTAGATGACCGGACCAACAACATCATCGCCTACCAGACGGGGGAGCGTTTGGAAGAGTTGCGGCGCATCGT
CAACCAACTGGATGTCCCGGTACGCCAGGTGATGATCGAGGCACGAATAGTCGAAGCCAATGTCGACTATGACAAAAGCG
TAGGGGTGCGCTGGGGCGGGTTGCTGCGGAATAAAGGCAATTGGAGTGGTGGTGGCGTCGACAACAGCCCGTCAGGTGAG
GGCGAGTCGCCCGATAGCTCGCCGTTCGTTGACCTGGGGGTCGTTAACGCAAGCTCTGGCCTGGGTATCGCGTTTGTTAC
CGATAACGTGTTGCTGGACCTGGAACTCACGGCCATGGAAAAAGGCGGCAATGGTGAAGTGATCTCCCAGCCCAAGGTGG
TGACCTCCGACAAGGAGACGGCGCGGATTCTCAAGGGCACTGAGATTCCTTACCAGGAAGCCACGTCCGAAGGGGCGACT
TCGGTTTCGTTCAAGGAAGCCTCGCTTTCCTTGGAAGTGACGCCACAAATCACTCCGGATGGCCAGGTCATCATGGAGGT
CCGGGTGACCAAGGATGAGCCGGACTACCTGAACAAACTCAACGATGTGCCGCCTATCAAGAAGAATGAGGTCAACGCCA
AGGTCCAGGTCAAGGACGGCGAGACCATCGTCCTCGGTGGGGTTTTCTCCAAAAACCAAAGCAAGGTGGTAGATAAAGTG
CCATTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTCGTGGCCCAAAGAAAATCCGAGCTGCTGGT
ATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(57-103)

Bacterial type II and III secretion system protein (Secretin)

(278-434)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.318

99.323

0.688
  pilQ Acinetobacter baumannii strain A118

41.72

100

0.438
  pilQ Acinetobacter baumannii D1279779

41.72

100

0.438
  comQ Acinetobacter baylyi ADP1

42.572

100

0.433
  pilQ Legionella pneumophila strain ERS1305867

44.286

94.808

0.42
  pilQ Legionella pneumophila strain Lp02

44.286

94.808

0.42
  pilQ Vibrio campbellii strain DS40M4

43.632

95.711

0.418
  pilQ Vibrio cholerae strain A1552

43.914

94.582

0.415
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.914

94.582

0.415

Multiple sequence alignment