Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HZ99_RS12515 Genome accession   NZ_CP008896
Coordinates   2847751..2848971 (+) Length   406 a.a.
NCBI ID   WP_038443437.1    Uniprot ID   A0A379IJG9
Organism   Pseudomonas fluorescens strain UK4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2842751..2853971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ99_RS12490 (HZ99_12790) nadC 2844183..2845031 (+) 849 WP_038443431.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HZ99_RS12495 (HZ99_12795) - 2845099..2845404 (-) 306 WP_038443433.1 DUF6388 family protein -
  HZ99_RS12505 (HZ99_12805) - 2845653..2847059 (-) 1407 WP_038443434.1 O-antigen ligase family protein -
  HZ99_RS12510 (HZ99_12810) - 2847137..2847547 (-) 411 WP_038443435.1 pilin -
  HZ99_RS12515 (HZ99_12815) pilC 2847751..2848971 (+) 1221 WP_038443437.1 type II secretion system F family protein Machinery gene
  HZ99_RS12520 (HZ99_12820) pilD 2848974..2849846 (+) 873 WP_038443439.1 prepilin peptidase Machinery gene
  HZ99_RS12525 (HZ99_12825) coaE 2849843..2850466 (+) 624 WP_038443440.1 dephospho-CoA kinase -
  HZ99_RS12530 (HZ99_12830) yacG 2850463..2850666 (+) 204 WP_038443442.1 DNA gyrase inhibitor YacG -
  HZ99_RS12535 (HZ99_12835) - 2850663..2850878 (-) 216 WP_038443443.1 hypothetical protein -
  HZ99_RS12540 (HZ99_12840) - 2850942..2851631 (-) 690 WP_038443445.1 energy-coupling factor ABC transporter permease -
  HZ99_RS12545 (HZ99_12845) - 2851628..2852095 (-) 468 WP_038443446.1 hypothetical protein -
  HZ99_RS12550 (HZ99_12850) - 2852198..2852827 (+) 630 WP_038443448.1 DUF1780 domain-containing protein -
  HZ99_RS12555 - 2852867..2853040 (+) 174 WP_038443450.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44055.86 Da        Isoelectric Point: 10.2258

>NTDB_id=125520 HZ99_RS12515 WP_038443437.1 2847751..2848971(+) (pilC) [Pseudomonas fluorescens strain UK4]
MNDVTVPTTALYAWEGTLPNGRNVSGEARGHSPAVIKAQLRRQGINPVRVQLRSSLLSGLGNPITPADIALFTRQLATLL
KAGIPLLQAFDMIGEGFDSRAMRMMVQGLKQEIAGGNSLTGALLKQPQYFNALYCSLIAAGEQAGTLETLLERVATHLEK
SQRLKARIKKAMTYPLVVLLVATLVSSILLIHVVPRFESLFAGIDTPLPRFTLLVIVLSEFMQGAWWMLLLAVTAIAWGV
RRSYRRYSGFRLWLDAGLLKLPLAGTLLKKTAVARYARTLATTFAAGVPLTQALDSVAGAAGNERFKRAITRMRQDVAIG
MQLNRTMTASGLFPGMAIQMTAIGEESGALDSMLEKVADHYETDVDNLVDNLTSLMEPLIMVVLGSIVGALVIAMYLPIF
QLGTAF

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=125520 HZ99_RS12515 WP_038443437.1 2847751..2848971(+) (pilC) [Pseudomonas fluorescens strain UK4]
ATGAACGATGTCACCGTCCCCACCACCGCCCTCTACGCCTGGGAAGGCACGTTACCCAATGGCCGTAATGTATCCGGAGA
AGCCCGTGGCCATAGCCCCGCAGTGATCAAGGCACAGTTGCGCCGGCAAGGTATCAACCCGGTACGGGTACAGCTACGCT
CCAGCCTCCTTTCTGGCCTGGGCAACCCAATCACCCCAGCCGATATTGCACTGTTCACTCGCCAACTGGCGACTCTGCTG
AAGGCGGGAATTCCCCTGCTGCAAGCCTTCGATATGATTGGCGAAGGCTTTGACAGCCGGGCCATGCGCATGATGGTCCA
GGGATTGAAGCAGGAGATTGCCGGCGGCAACAGCCTGACCGGAGCGCTGCTGAAACAACCACAGTATTTTAATGCCCTAT
ATTGCAGCCTGATCGCGGCAGGGGAACAGGCCGGCACCCTGGAAACCCTGCTGGAACGGGTAGCCACCCATCTGGAGAAG
AGCCAACGCCTCAAGGCCCGGATCAAGAAGGCCATGACCTATCCGCTGGTGGTCCTGCTCGTGGCTACGCTAGTCAGCAG
CATTTTGCTGATCCATGTCGTGCCCCGGTTCGAAAGCCTGTTCGCCGGTATCGACACACCTCTGCCGCGCTTTACCCTGC
TGGTGATCGTGCTGTCCGAATTCATGCAAGGCGCGTGGTGGATGCTGTTGCTGGCCGTGACCGCAATTGCCTGGGGAGTA
CGCCGCAGCTACCGCCGATACAGCGGTTTTCGCCTCTGGCTGGACGCAGGTTTGTTGAAACTGCCGCTGGCAGGCACACT
GCTGAAAAAAACCGCAGTCGCCCGCTACGCCCGCACACTCGCCACCACTTTTGCCGCCGGCGTGCCGCTGACACAAGCGT
TGGATTCGGTGGCCGGAGCCGCCGGCAACGAGCGATTCAAACGAGCGATCACACGTATGCGCCAGGACGTGGCAATAGGA
ATGCAATTGAATCGAACCATGACCGCCAGCGGCCTGTTCCCTGGCATGGCGATCCAGATGACAGCCATAGGCGAAGAATC
GGGCGCGCTGGACAGCATGCTGGAGAAGGTCGCCGATCATTATGAAACAGACGTCGACAACCTGGTCGATAACCTGACCA
GCCTGATGGAGCCGCTGATCATGGTCGTACTCGGCAGCATTGTCGGGGCACTGGTAATTGCCATGTACCTGCCGATCTTC
CAACTCGGCACGGCGTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(277-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379IJG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

97.537

0.559
  pilC Acinetobacter baylyi ADP1

52.02

97.537

0.507
  pilC Acinetobacter baumannii D1279779

50.886

97.291

0.495
  pilC Legionella pneumophila strain ERS1305867

46.914

99.754

0.468
  pilC Vibrio cholerae strain A1552

40.306

96.552

0.389
  pilG Neisseria gonorrhoeae MS11

40.157

93.842

0.377
  pilG Neisseria meningitidis 44/76-A

39.37

93.842

0.369
  pilC Vibrio campbellii strain DS40M4

37.783

97.783

0.369

Multiple sequence alignment