Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HV99_RS02025 Genome accession   NZ_CP008868
Coordinates   438735..439955 (+) Length   406 a.a.
NCBI ID   WP_023089631.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain T63266     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 433735..444955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV99_RS02000 (HV99_01985) - 434291..434587 (+) 297 Protein_391 helix-turn-helix domain-containing protein -
  HV99_RS29830 - 434556..435002 (-) 447 Protein_392 DUF1631 family protein -
  HV99_RS02005 (HV99_01990) nadC 435288..436136 (+) 849 WP_023089629.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HV99_RS02015 (HV99_02000) pilA 436350..436799 (-) 450 WP_031627735.1 type 4a pilus biogenesis protein PilA Machinery gene
  HV99_RS02020 (HV99_02005) pilB 437031..438731 (+) 1701 WP_023089630.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HV99_RS02025 (HV99_02010) pilC 438735..439955 (+) 1221 WP_023089631.1 type 4a pilus biogenesis protein PilC Machinery gene
  HV99_RS02030 (HV99_02015) pilD 439959..440831 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HV99_RS02035 (HV99_02020) coaE 440828..441439 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HV99_RS02040 (HV99_02025) yacG 441436..441636 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HV99_RS02045 (HV99_02030) - 441673..441882 (-) 210 WP_003094660.1 hypothetical protein -
  HV99_RS02050 (HV99_02035) - 441988..442677 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  HV99_RS02055 (HV99_02040) - 442674..443144 (-) 471 WP_003103866.1 hypothetical protein -
  HV99_RS02060 (HV99_02045) - 443141..443566 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  HV99_RS02065 (HV99_02050) - 443699..444328 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  HV99_RS02070 (HV99_02055) - 444325..444774 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44360.15 Da        Isoelectric Point: 9.8784

>NTDB_id=125156 HV99_RS02025 WP_023089631.1 438735..439955(+) (pilC) [Pseudomonas aeruginosa strain T63266]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVAVGVIGFTFK
ELHKRSKKFRDTLDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=125156 HV99_RS02025 WP_023089631.1 438735..439955(+) (pilC) [Pseudomonas aeruginosa strain T63266]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGT
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTAATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTCCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAATGGTGGCTTGCCGTCATCGTTGCCGTGGGGGTGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCAAAAAAATTCCGCGACACACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCTGCGGTCGCTCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.79

99.754

0.766
  pilC Acinetobacter baumannii D1279779

60.891

99.507

0.606
  pilC Acinetobacter baylyi ADP1

60.345

100

0.603
  pilC Legionella pneumophila strain ERS1305867

54.798

97.537

0.534
  pilG Neisseria meningitidis 44/76-A

45.906

99.261

0.456
  pilG Neisseria gonorrhoeae MS11

45.658

99.261

0.453
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

36.908

98.768

0.365

Multiple sequence alignment