Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HW03_RS22620 Genome accession   NZ_CP008863
Coordinates   4892699..4893916 (-) Length   405 a.a.
NCBI ID   WP_016254080.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain M37351     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4887699..4898916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW03_RS22580 - 4888084..4888404 (+) 321 Protein_4532 FAD/FMN-containing dehydrogenase -
  HW03_RS22585 (HW03_22810) - 4888410..4889711 (-) 1302 WP_011920678.1 ISL3-like element IS1411 family transposase -
  HW03_RS22590 (HW03_22815) - 4889805..4889981 (+) 177 Protein_4534 FAD/FMN-containing dehydrogenase -
  HW03_RS22595 (HW03_22820) - 4889978..4890667 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  HW03_RS22600 (HW03_22825) - 4890773..4890982 (+) 210 WP_003094660.1 hypothetical protein -
  HW03_RS22605 (HW03_22830) yacG 4891019..4891219 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HW03_RS22610 (HW03_22835) coaE 4891216..4891827 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  HW03_RS22615 (HW03_22840) pilD 4891824..4892696 (-) 873 WP_003141356.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HW03_RS22620 (HW03_22845) pilC 4892699..4893916 (-) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene
  HW03_RS22625 (HW03_22850) pilB 4893920..4895623 (-) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HW03_RS22630 - 4895852..4896010 (+) 159 Protein_4542 pilin -
  HW03_RS22635 (HW03_22860) - 4896026..4897327 (-) 1302 WP_011920678.1 ISL3-like element IS1411 family transposase -
  HW03_RS32935 - 4897435..4897818 (+) 384 WP_257721537.1 pilin -
  HW03_RS22640 (HW03_22865) - 4897882..4898580 (+) 699 WP_016254081.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44041.92 Da        Isoelectric Point: 9.8417

>NTDB_id=125011 HW03_RS22620 WP_016254080.1 4892699..4893916(-) (pilC) [Pseudomonas aeruginosa strain M37351]
MADKALKTSVFIWEGTDKKGSKVKGELAGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMSTMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQDVAAGNSLASSLRKKPIYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEMLQEWWLIVLIGLFAAAFAFR
EAHRRSQKLRDSVDRGLLKLPIIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVTKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=125011 HW03_RS22620 WP_016254080.1 4892699..4893916(-) (pilC) [Pseudomonas aeruginosa strain M37351]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGCACTGATAAGAAAGGCAGCAAGGTCAAAGGAGAGTT
GGCCGGGCAAAACCCTATGCTGGTGAAGGCTCAACTGCGCAAACAGGGTATCAACCCACTAAAGGTCCGCAAGAAAGGTA
TCACCCTGTTTGGGGCAGGGAAGAAGATTAAGCCCATGGACATCGCCCTGTTCACTCGGCAGATGTCTACCATGATGGGT
GCCGGCGTACCGTTACTGCAATCTTTTGACATCATCGGCGAAGGATTCGAAAATCCAAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGATGTTGCCGCCGGTAACAGCTTAGCCAGTTCACTTCGAAAGAAACCCATTTACTTCGATGATCTCTACT
GCAACCTGGTCGATGCTGGCGAACAGTCCGGTGCTTTGGAGACATTATTGGATCGGGTAGCAACTTATAAAGAAAAGACA
GAATCCCTGAAAGCCAAAATTAAAAAAGCCATGACTTATCCCATTGCAGTAATTGTAGTGGCCCTTGTGGTATCGGCGAT
CCTTCTGATAAAAGTGGTCCCACAGTTCCAGTCCGTATTTGCAAATTTTGGTGCCGAGTTGCCGGCCTTTACTCAAATGG
TCATCAATCTTTCCGAGATGCTTCAAGAGTGGTGGCTCATAGTGCTTATTGGTCTTTTTGCCGCAGCTTTTGCATTTAGG
GAAGCTCATCGGCGCTCGCAAAAACTGAGAGATTCAGTAGATCGGGGCCTGCTGAAACTACCTATCATCGGCGGGATACT
TTACAAATCAGCTATCGCCCGCTACGCCCGAACGCTATCCACTACCTTTGCGGCTGGAGTGCCTCTGGTAGAAGCTCTGG
ACTCCGTTTCCGGAGCAACTGGCAACGTGGTATTCAAGAATGCCGTTACCAAGATCAAACAAGACGTGTCCAGCGGCATG
CAATTGAACTTTTCCATGCGCACCACCAATGTTTTCCCCAGTATGGCTATCCAGATGGCTGCAATTGGCGAGGAATCAGG
TTCGCTAGATGAGATGTTAGGAAAAGTTGCCGGTTTCTATGAGGAAGAAGTCGATAACGCCGTCGACAACCTGACAACGT
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.753

100

0.798
  pilC Acinetobacter baumannii D1279779

60.891

99.753

0.607
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Legionella pneumophila strain ERS1305867

55.303

97.778

0.541
  pilG Neisseria meningitidis 44/76-A

46.269

99.259

0.459
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment