Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CF65_RS00540 Genome accession   NZ_CP007502
Coordinates   113524..114933 (+) Length   469 a.a.
NCBI ID   WP_019518272.1    Uniprot ID   Q9JRM9
Organism   Aggregatibacter actinomycetemcomitans HK1651     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 108524..119933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CF65_RS00520 (CF65_00151) pnp 109002..111131 (-) 2130 WP_025298076.1 polyribonucleotide nucleotidyltransferase -
  CF65_RS00525 (CF65_00153) nanQ 111425..111895 (+) 471 WP_005547536.1 N-acetylneuraminate anomerase -
  CF65_RS00530 (CF65_00158) ampD 112369..112923 (-) 555 WP_005567973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CF65_RS00535 (CF65_00160) pilA 113045..113497 (+) 453 WP_005586498.1 pilin Machinery gene
  CF65_RS00540 (CF65_00161) pilB 113524..114933 (+) 1410 WP_019518272.1 GspE/PulE family protein Machinery gene
  CF65_RS00545 (CF65_00162) pilC 114926..116149 (+) 1224 WP_025298077.1 type II secretion system F family protein Machinery gene
  CF65_RS00550 (CF65_00163) - 116146..116835 (+) 690 WP_005547547.1 prepilin peptidase -
  CF65_RS00555 (CF65_00164) coaE 116885..117508 (+) 624 WP_005547548.1 dephospho-CoA kinase -
  CF65_RS00560 (CF65_00165) yacG 117498..117710 (+) 213 WP_005567966.1 DNA gyrase inhibitor YacG -
  CF65_RS00565 (CF65_00166) - 117710..117982 (+) 273 WP_005547552.1 GNAT family N-acetyltransferase -
  CF65_RS00570 (CF65_00167) - 118379..119752 (+) 1374 WP_005547554.1 alanine/glycine:cation symporter family protein -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52549.85 Da        Isoelectric Point: 6.2867

>NTDB_id=120048 CF65_RS00540 WP_019518272.1 113524..114933(+) (pilB) [Aggregatibacter actinomycetemcomitans HK1651]
MQNPMAQGYLIHSQNHGDFTIAPELWLKNQQQQTVLLRYLALPLAENGQTLWLGLDSLTNLSACEAFSFLTGKTIEPVLI
ESQTLKTALQDLSPRVEKVEENQPLFYSALSQEQPTQPYDEPIIRLLNDIFESATAQKASDIHLEPQADCLQTRFRIDGV
LQVQNTVPLSIASRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDTLDFRLSTLATQFGEKAVLRLQQNRPVQLAFGEL
GMTESQQQRFRRALSQPQGLILVTGPTGSGKSISLYTALQYLNTSEKHIMTAEDPIEIQLPGIIQSQVNHPIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAPSAISRLQQLGIQRHEIDGSLLLVIAQRLVRKRCQK
CGGNATRFCDCHQGYKGRVGVYQFLQPNLQHPQGYETDFADLGQSALEKLKDGTTDLTEIQRVLGQTHD

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=120048 CF65_RS00540 WP_019518272.1 113524..114933(+) (pilB) [Aggregatibacter actinomycetemcomitans HK1651]
ATGCAAAATCCGATGGCGCAAGGTTATTTGATTCACAGCCAAAATCACGGGGACTTTACCATTGCGCCCGAGCTATGGCT
TAAAAATCAGCAACAACAAACCGTCCTGTTGCGTTATCTCGCCCTGCCCTTAGCAGAAAACGGGCAGACCTTGTGGCTAG
GGTTAGACTCGTTAACTAACCTGAGCGCCTGTGAAGCCTTTTCTTTTCTCACCGGCAAAACCATTGAGCCGGTATTAATT
GAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTA
TTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCG
CGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTG
CTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATAT
TAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCA
CCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTGCAACTGGCATTCGGTGAACTG
GGCATGACGGAATCACAGCAACAGCGTTTTCGTCGTGCCTTGAGCCAACCACAAGGCTTGATTTTAGTCACCGGTCCGAC
AGGCAGCGGCAAAAGTATTTCTTTATATACGGCGTTGCAGTACTTAAATACGTCTGAAAAACACATTATGACGGCGGAAG
ATCCCATTGAAATTCAGCTCCCAGGCATTATTCAAAGCCAAGTGAATCACCCTATCGGCTTGGATTTCAGCCGATTGCTT
CGCACCTTTTTACGCCAAGATCCGGACATTATTATGTTAGGTGAAATTCGTGATGAAGAAAGTGCCGCCATGGCATTACG
CGCCGCGCAAACGGGGCATTTGGTGCTTTCGACGCTACATACCAACGACGCCCCTTCCGCCATTTCCCGCCTGCAACAAC
TCGGCATTCAACGCCATGAAATCGACGGCAGTTTGTTGCTGGTGATCGCCCAACGTTTGGTGCGCAAACGCTGCCAAAAG
TGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACC
GAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCA
CAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9JRM9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

69.427

100

0.697
  pilB Haemophilus influenzae 86-028NP

70.87

98.081

0.695
  pilB Glaesserella parasuis strain SC1401

57.328

98.934

0.567
  pilB Vibrio cholerae strain A1552

40.53

100

0.424
  pilB Acinetobacter baylyi ADP1

39.078

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

40.041

100

0.412
  pilB Vibrio campbellii strain DS40M4

39.752

100

0.409
  pilB Acinetobacter baumannii D1279779

38.855

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.331

100

0.401
  pilF Neisseria gonorrhoeae MS11

36

100

0.365

Multiple sequence alignment