Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CF65_RS00535 Genome accession   NZ_CP007502
Coordinates   113045..113497 (+) Length   150 a.a.
NCBI ID   WP_005586498.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans HK1651     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 108045..118497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CF65_RS00520 (CF65_00151) pnp 109002..111131 (-) 2130 WP_025298076.1 polyribonucleotide nucleotidyltransferase -
  CF65_RS00525 (CF65_00153) nanQ 111425..111895 (+) 471 WP_005547536.1 N-acetylneuraminate anomerase -
  CF65_RS00530 (CF65_00158) ampD 112369..112923 (-) 555 WP_005567973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CF65_RS00535 (CF65_00160) pilA 113045..113497 (+) 453 WP_005586498.1 pilin Machinery gene
  CF65_RS00540 (CF65_00161) pilB 113524..114933 (+) 1410 WP_019518272.1 GspE/PulE family protein Machinery gene
  CF65_RS00545 (CF65_00162) pilC 114926..116149 (+) 1224 WP_025298077.1 type II secretion system F family protein Machinery gene
  CF65_RS00550 (CF65_00163) - 116146..116835 (+) 690 WP_005547547.1 prepilin peptidase -
  CF65_RS00555 (CF65_00164) coaE 116885..117508 (+) 624 WP_005547548.1 dephospho-CoA kinase -
  CF65_RS00560 (CF65_00165) yacG 117498..117710 (+) 213 WP_005567966.1 DNA gyrase inhibitor YacG -
  CF65_RS00565 (CF65_00166) - 117710..117982 (+) 273 WP_005547552.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15547.96 Da        Isoelectric Point: 9.1384

>NTDB_id=120047 CF65_RS00535 WP_005586498.1 113045..113497(+) (pilA) [Aggregatibacter actinomycetemcomitans HK1651]
MKIQSAFISLKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGVITVTGKGSLDKISYTLTASGTAASGVSWNAACGNNADIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=120047 CF65_RS00535 WP_005586498.1 113045..113497(+) (pilA) [Aggregatibacter actinomycetemcomitans HK1651]
ATGAAAATTCAATCCGCGTTTATTTCGCTAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATCGC
TATTGTTGCTATTCTCGCCACCGTAGCCGTACCATCTTATCAAAATTACACTAAAAAAGCGGCGGTTTCCGAATTATTAC
AAGCCTCCGCCCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AACGGCATTCAAGCAGATGTTGCAGATGCTTCGAAACAAAAATATGTGAAATCCACTGCGGTCAAAGGCGGCGTCATCAC
CGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGGCACGGCTGCCAGCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGACATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.319

92

0.573
  pilA Haemophilus influenzae Rd KW20

59.028

96

0.567
  pilA Glaesserella parasuis strain SC1401

53.846

95.333

0.513

Multiple sequence alignment