Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CD58_RS24310 Genome accession   NZ_CP007410
Coordinates   5677879..5679096 (+) Length   405 a.a.
NCBI ID   WP_025215526.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain DF41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5672879..5684096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CD58_RS24290 (CD58_25250) - 5674223..5674519 (+) 297 WP_025215522.1 DUF2845 domain-containing protein -
  CD58_RS24295 (CD58_25255) - 5674857..5675210 (-) 354 WP_025215523.1 BON domain-containing protein -
  CD58_RS24300 (CD58_25260) - 5675522..5675950 (-) 429 WP_025215524.1 pilin -
  CD58_RS24305 (CD58_25265) pilB 5676176..5677876 (+) 1701 WP_025215525.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CD58_RS24310 (CD58_25270) pilC 5677879..5679096 (+) 1218 WP_025215526.1 type II secretion system F family protein Machinery gene
  CD58_RS24315 (CD58_25275) pilD 5679098..5679967 (+) 870 WP_025215527.1 prepilin peptidase Machinery gene
  CD58_RS24320 (CD58_25280) coaE 5679980..5680603 (+) 624 WP_025215528.1 dephospho-CoA kinase -
  CD58_RS24325 (CD58_25285) yacG 5680600..5680806 (+) 207 WP_025215529.1 DNA gyrase inhibitor YacG -
  CD58_RS24330 (CD58_25290) - 5680827..5681042 (-) 216 WP_003185533.1 hypothetical protein -
  CD58_RS24335 (CD58_25295) - 5681106..5681792 (-) 687 WP_025215530.1 energy-coupling factor ABC transporter permease -
  CD58_RS24340 (CD58_25300) - 5682024..5682650 (+) 627 WP_025215531.1 DUF1780 domain-containing protein -
  CD58_RS24345 (CD58_25305) - 5682647..5683174 (+) 528 WP_025215532.1 MOSC domain-containing protein -
  CD58_RS24350 - 5683227..5683400 (+) 174 WP_003185543.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44216.81 Da        Isoelectric Point: 9.9531

>NTDB_id=119272 CD58_RS24310 WP_025215526.1 5677879..5679096(+) (pilC) [Pseudomonas brassicacearum strain DF41]
MAVKAVKTDVYTWEGKDRKGTKMSGELTGQSPALIKAQLRKQGINPEKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNANMRKLVDEVKQEVAAGNSFAASLRKCPQYFDDLYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVVVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEFLQQWWWVVLGGFVGTFFGVK
YALKRSQGFRDWRDKWLLKLPLIGSLMYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIRQDVSTGM
QLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=119272 CD58_RS24310 WP_025215526.1 5677879..5679096(+) (pilC) [Pseudomonas brassicacearum strain DF41]
ATGGCGGTCAAAGCAGTAAAAACCGATGTCTACACGTGGGAAGGCAAAGACCGCAAAGGCACGAAAATGAGCGGTGAACT
GACCGGTCAGAGCCCAGCCCTGATCAAGGCTCAGTTACGCAAACAGGGCATCAACCCGGAGAAGGTACGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGTTGGACATCGCCCTGTTCACCCGCCAGATGGCGACGATGCTCAAG
GCCGGCGTGCCGCTGTTGCAGGCGTTCGACATCATTGGCGAAGGCTTCGACAACGCTAATATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCTGCCGGCAACAGCTTCGCGGCGTCGTTGCGAAAATGTCCGCAGTATTTCGATGACTTGTACT
GCAACCTGGTAGACGCCGGTGAACAGGCCGGTGCCCTGGACACGCTGCTGGATCGGGTCGCGACCTACAAGGAAAAGAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGGCGGCCGTAGTGGTGGTCGCGGCCGTGGTCACCGGCAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCGAATCGGTCTTTTCAGGATTCGGCGCACAGCTACCGGCCTTCACGGTGATGG
TCATCGGCCTGTCGGAATTCCTGCAGCAATGGTGGTGGGTGGTGCTTGGCGGGTTTGTGGGGACGTTTTTCGGCGTGAAA
TACGCGCTCAAGCGCTCCCAGGGGTTTCGCGACTGGCGCGATAAATGGCTGCTCAAGCTGCCGCTGATAGGCAGCTTGAT
GTACAAGTCCGCTGTGGCCCGCTACGCCCGGACGCTGTCCACCACATTCGCCGCCGGCGTGCCGCTGGTAGAGGCACTGG
ATTCGGTCTCGGGGGCCACCGGTAACGTGGTGTTCAAACGCGCGGTGCAGCGCATCCGCCAGGATGTCTCGACCGGCATG
CAGTTGAATTTTTCCATGCGTGCGTCAGGCATCTTTCCCAACCTGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
CGCGCTGGACGACATGCTCGACAAGGTGGCGAGTTTTTATGAGGCCGAGGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTGGTCGTCGGTGGCCTGGTGGTTGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.815

100

0.748
  pilC Acinetobacter baumannii D1279779

57.843

100

0.583
  pilC Acinetobacter baylyi ADP1

59.296

98.272

0.583
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilC Vibrio campbellii strain DS40M4

43.939

97.778

0.43
  pilG Neisseria meningitidis 44/76-A

42.469

100

0.425
  pilG Neisseria gonorrhoeae MS11

42.537

99.259

0.422
  pilC Vibrio cholerae strain A1552

42.929

97.778

0.42
  pilC Thermus thermophilus HB27

37.656

99.012

0.373

Multiple sequence alignment