Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACOCLE_RS14325 Genome accession   NZ_OZ239981
Coordinates   3169648..3171684 (-) Length   678 a.a.
NCBI ID   WP_419533037.1    Uniprot ID   -
Organism   MAG: Endozoicomonas sp. isolate 83fe6ec3-ea9b-4b46-8e1a-20fbcf80582c     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3164648..3176684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOCLE_RS14315 aroB 3167906..3168985 (-) 1080 WP_419533035.1 3-dehydroquinate synthase -
  ACOCLE_RS14320 aroK 3169000..3169521 (-) 522 WP_419533036.1 shikimate kinase AroK -
  ACOCLE_RS14325 pilQ 3169648..3171684 (-) 2037 WP_419533037.1 type IV pilus secretin PilQ Machinery gene
  ACOCLE_RS14330 - 3171945..3172481 (-) 537 WP_419533038.1 pilus assembly protein PilP -
  ACOCLE_RS14335 - 3172478..3173089 (-) 612 WP_419533039.1 type 4a pilus biogenesis protein PilO -
  ACOCLE_RS14340 - 3173086..3173649 (-) 564 WP_419533040.1 PilN domain-containing protein -
  ACOCLE_RS14345 pilM 3173649..3174713 (-) 1065 WP_419533041.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 678 a.a.        Molecular weight: 73507.57 Da        Isoelectric Point: 4.5133

>NTDB_id=1170739 ACOCLE_RS14325 WP_419533037.1 3169648..3171684(-) (pilQ) [MAG: Endozoicomonas sp. isolate 83fe6ec3-ea9b-4b46-8e1a-20fbcf80582c]
MNISPLLQAKAVRGLLYTLLAMLPISAWGVTLKNMDVSALPGDMVELRLTFDGAAPVAHGYSIEKPPRISIDLPFTRSEL
PKYNEVGFNNAKSVTVLEAGDRTRLVINLQQPTRFTSQADGRTLSIYLGDDNSTAAIQRESVQEKSPQDTGVSKGITDID
FKRGDDGEGEIIIALANAKVPMDMNEQGGRIRLEFQGEVLPQHLRNRLDVIDFATPVKFIDAMVEDGNSIIVIEPKGEFE
YLAYQTDNLLTVSVKAPDRKDQSRRKAGLSYKGDKLSLNFQDIEVRAVLQLIADFTDLNLVASDTVGGNVTLRLQNVPWD
QALDIVLKAKGLDKRLEGNVLTVAPAAEIAAREREQLENEKQIRELAPVYTDLVQINYADAAEVAEVLQGSADATLLTER
GSVQVVARTNSLLIKDTQAKLDELRALIEQLDIPIRQVMIEARIVTLSSDFKEELGVKWSGKKNDALKNDDRGLSLGGGQ
DGDVFTDLGVTGGGSIAIAIGFSTNGGTILNLELSALLSDGGGEVISQPKVITADKKTAVIKSGKEIPYEEKTSSGATSV
AFKDAVLGLEVTPQITPDGRVIMDIKINNDDTTDQASNNVPIISTNEITTQVLVEDGETVVLGGVFKQTKQQGVTKVPVL
GDMPFVGALFRKNTESDTKEELLVFITPRIVTEGVSLR

Nucleotide


Download         Length: 2037 bp        

>NTDB_id=1170739 ACOCLE_RS14325 WP_419533037.1 3169648..3171684(-) (pilQ) [MAG: Endozoicomonas sp. isolate 83fe6ec3-ea9b-4b46-8e1a-20fbcf80582c]
ATGAATATTAGTCCCCTGTTGCAGGCGAAGGCTGTTCGAGGGCTTCTGTATACGCTGCTGGCCATGTTGCCTATTTCCGC
GTGGGGTGTCACTCTGAAGAATATGGATGTCTCTGCGTTGCCAGGTGACATGGTTGAGCTCCGTTTAACCTTTGATGGGG
CTGCCCCCGTTGCTCATGGTTACAGTATTGAGAAGCCACCAAGGATTTCCATAGACCTGCCTTTTACCCGCAGTGAACTC
CCAAAATACAATGAGGTTGGCTTTAATAACGCCAAAAGTGTCACAGTGCTTGAAGCGGGTGACCGAACTCGTCTGGTTAT
TAATCTTCAGCAGCCTACCCGCTTTACCAGTCAGGCCGATGGAAGAACGCTCAGTATTTATCTGGGGGATGACAATAGTA
CTGCGGCGATTCAAAGGGAGTCGGTTCAGGAAAAATCACCCCAAGATACAGGCGTGAGCAAAGGCATTACGGATATTGAC
TTTAAGCGCGGTGATGATGGTGAAGGGGAAATCATCATAGCACTGGCTAATGCCAAAGTACCTATGGATATGAATGAGCA
GGGTGGACGTATTCGTTTGGAATTCCAGGGAGAGGTCTTGCCTCAGCACCTGAGAAACCGTCTGGACGTTATTGACTTTG
CCACACCGGTCAAATTTATCGATGCGATGGTTGAGGATGGGAACAGCATCATCGTTATTGAGCCAAAGGGCGAGTTCGAA
TACCTGGCTTATCAGACGGACAATTTATTAACTGTTAGTGTCAAGGCTCCAGACAGAAAAGATCAGAGCAGGAGAAAAGC
CGGGCTTTCTTACAAGGGCGACAAACTGTCGTTGAATTTTCAGGATATTGAAGTAAGAGCAGTTCTACAGCTGATTGCTG
ATTTTACAGACTTGAATCTGGTGGCATCCGATACCGTTGGGGGCAATGTTACCCTGCGTCTGCAGAACGTTCCATGGGAT
CAGGCTCTTGATATCGTACTTAAGGCCAAAGGGCTCGATAAACGGCTTGAAGGTAATGTTTTGACGGTGGCTCCAGCTGC
TGAAATTGCGGCTCGAGAGCGTGAACAGCTTGAGAATGAAAAGCAGATTCGTGAATTGGCGCCTGTATATACAGATCTTG
TTCAGATTAATTATGCAGATGCCGCTGAGGTTGCAGAAGTGCTTCAGGGAAGTGCAGATGCAACATTACTCACCGAAAGA
GGCTCTGTTCAGGTGGTTGCCAGAACCAATAGCCTGCTGATCAAAGACACTCAGGCAAAACTGGATGAATTGAGAGCGCT
GATTGAGCAGCTGGATATCCCTATCCGACAGGTAATGATTGAGGCTCGGATTGTCACGCTCAGTTCTGATTTCAAAGAAG
AGCTGGGGGTAAAATGGAGCGGTAAAAAGAATGATGCGCTCAAAAACGACGACAGAGGCCTTTCACTGGGTGGTGGTCAG
GATGGCGATGTCTTTACTGACTTAGGCGTTACTGGCGGAGGCTCAATTGCTATTGCCATCGGGTTCTCAACGAATGGCGG
TACCATATTGAACCTGGAGCTGTCGGCTTTGTTGAGTGATGGTGGTGGTGAAGTTATTTCCCAGCCAAAGGTGATTACCG
CTGATAAGAAAACGGCGGTGATCAAGTCAGGTAAAGAAATCCCGTACGAAGAAAAAACCTCCAGTGGTGCAACGTCTGTA
GCCTTTAAGGATGCTGTACTTGGATTGGAGGTAACGCCTCAGATTACACCGGATGGTCGGGTTATCATGGATATCAAAAT
CAATAATGATGACACTACCGATCAGGCCTCCAATAATGTCCCCATTATTTCAACCAATGAGATTACGACTCAGGTATTGG
TTGAAGATGGTGAAACGGTAGTTTTGGGTGGGGTGTTTAAACAGACTAAACAGCAAGGGGTAACCAAAGTTCCAGTTCTA
GGAGATATGCCTTTTGTTGGAGCCTTATTTAGGAAGAACACAGAGAGCGATACAAAAGAGGAACTGCTGGTCTTCATTAC
CCCAAGAATTGTAACTGAAGGTGTTTCTCTGCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(517-671)

Secretin and TonB N terminus short domain (STN)

(299-345)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

48.291

100

0.5
  comQ Acinetobacter baylyi ADP1

41.32

100

0.425
  pilQ Acinetobacter baumannii D1279779

40.169

100

0.422
  pilQ Acinetobacter baumannii strain A118

40.169

100

0.422
  pilQ Legionella pneumophila strain Lp02

38.797

98.083

0.381
  pilQ Legionella pneumophila strain ERS1305867

38.647

98.083

0.379

Multiple sequence alignment