Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNALX_RS01800 Genome accession   NZ_OZ223210
Coordinates   357412..359112 (-) Length   566 a.a.
NCBI ID   WP_415719994.1    Uniprot ID   -
Organism   MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 352412..364112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNALX_RS01770 mutT 352887..353285 (+) 399 WP_047886947.1 8-oxo-dGTP diphosphatase MutT -
  ACNALX_RS01775 yacG 353378..353596 (-) 219 WP_047887028.1 DNA gyrase inhibitor YacG -
  ACNALX_RS01780 zapD 353605..354342 (-) 738 WP_047886946.1 cell division protein ZapD -
  ACNALX_RS01785 coaE 354367..354981 (-) 615 WP_047886945.1 dephospho-CoA kinase -
  ACNALX_RS01790 pilD 354986..355855 (-) 870 WP_273857551.1 prepilin peptidase Machinery gene
  ACNALX_RS01795 pilC 356138..357358 (-) 1221 WP_415719993.1 type II secretion system F family protein Machinery gene
  ACNALX_RS01800 pilB 357412..359112 (-) 1701 WP_415719994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNALX_RS01805 - 359112..359555 (-) 444 WP_217391891.1 pilin -
  ACNALX_RS01810 nadC 360029..360916 (-) 888 WP_415720045.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACNALX_RS01815 ampD 361216..361779 (+) 564 WP_047886939.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACNALX_RS01820 pdhR 362076..362849 (+) 774 WP_047886938.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62562.39 Da        Isoelectric Point: 5.3856

>NTDB_id=1170627 ACNALX_RS01800 WP_415719994.1 357412..359112(-) (pilB) [MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5]
MHTNLASILHQADLLSQVEQQQVVDQVQSEGISVPSALLQQGIIGSAELARQLEQLFALPLVDVHQYDYTECCKNLNLRD
LVLHHKVLPLYNTDTSIFIGLSDPTRIDVLDEFRFATGKNIEPLLLDHKQLEAAIRRVYGSDIKGAGANKTQRSVSDADL
MELVDLTDTELADDATDLTSDNAPVTRYINQVLQDALRKSASDIHLEPYEESYRIRFRCDGILHLHASPPANLSRRLSTR
LKVMSRLNIAERRQPQDGRIKLKLSENVAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYTEPQKAAYLSALQRPQ
GMILMTGPTGSGKTVSLYTGLKILNTEERNISTAEDPVEINLPGINQVQIRPTVGLGFAEALRSFLRQDPDVVMVGEIRD
LETGSIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIENFNLASSLSLIIAQRLARRLCGHCKQPDDPDRFIRDTLAIP
DNATIFKASPTGCDECNKGYLGRVGIYEVMPFSRELAEALMAGANTLELEDIACRQGMKTLQQSGIEKLCEGITSLKELQ
RVLHFS

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1170627 ACNALX_RS01800 WP_415719994.1 357412..359112(-) (pilB) [MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5]
ATGCACACGAACCTAGCCTCCATTCTTCATCAGGCAGACCTGCTCAGCCAGGTGGAGCAGCAACAAGTGGTGGATCAGGT
TCAGTCAGAGGGCATTTCGGTGCCCTCTGCACTGTTACAGCAGGGCATCATCGGCAGCGCCGAACTGGCCCGGCAACTGG
AACAGCTGTTCGCCCTGCCGCTGGTTGATGTCCATCAGTACGATTACACGGAATGTTGCAAAAACCTAAACCTACGGGAT
CTGGTGTTACACCACAAAGTGCTGCCGTTGTACAACACGGACACCAGCATTTTTATTGGCCTGAGCGATCCAACCCGCAT
TGATGTGCTGGATGAGTTCCGCTTTGCCACCGGCAAAAACATTGAGCCGCTATTGCTGGATCATAAGCAACTGGAAGCGG
CGATCCGCCGGGTATACGGCAGTGACATTAAAGGGGCCGGAGCCAACAAAACACAGCGCAGTGTCAGTGATGCGGATCTG
ATGGAACTGGTGGATCTGACCGACACCGAACTGGCTGACGACGCCACAGATCTCACCAGCGATAACGCCCCGGTAACCCG
CTATATCAACCAGGTGCTGCAGGACGCCCTGCGTAAAAGCGCTTCCGATATCCATTTGGAACCCTATGAAGAGAGCTATC
GGATCCGTTTTCGCTGCGACGGGATTTTGCATCTGCACGCCTCCCCGCCAGCCAATTTGTCCCGCCGCTTATCCACCCGG
CTGAAGGTCATGTCCAGGCTCAATATTGCCGAGCGGCGCCAGCCTCAGGATGGCCGGATCAAACTCAAATTGTCCGAAAA
TGTGGCCGTGGATTTGCGGGTTTCCACACTGCCTACCCTGTGGGGTGAAAAGGTGGTGCTGCGGATTCTTGACAGCTCCA
GTGCCAATCTGGATATCGATATTCTGGGTTATACCGAGCCCCAAAAAGCCGCATACCTGAGTGCCCTTCAGCGCCCTCAG
GGCATGATTCTGATGACCGGACCGACTGGCAGTGGCAAAACTGTCTCCCTGTATACCGGCCTCAAGATCCTGAACACCGA
GGAACGCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACCTGCCGGGCATAAATCAGGTACAGATACGCCCGACCG
TCGGGCTTGGGTTTGCAGAAGCACTGCGCTCATTCTTGCGCCAGGATCCGGATGTCGTCATGGTTGGGGAAATCAGGGAT
CTGGAAACCGGCTCCATTGCCGTGAAAGCGGCTCAGACCGGCCACCTAGTGTTGTCGACCCTGCACACCAACTCAGCGGC
GGAAACCATTACCCGCCTGACCAATATGGGGATCGAGAACTTCAACCTGGCATCGTCATTGAGCCTAATTATTGCTCAGC
GGCTGGCCCGCCGCCTTTGCGGACATTGCAAGCAGCCTGATGACCCGGATCGTTTTATCAGAGATACCCTCGCGATTCCC
GACAATGCCACCATTTTCAAAGCCAGCCCAACAGGGTGTGATGAGTGTAACAAAGGCTATCTGGGACGGGTCGGTATCTA
CGAGGTCATGCCCTTTTCACGAGAGCTCGCCGAAGCCTTAATGGCAGGTGCCAATACACTGGAGCTGGAAGACATTGCCT
GTCGGCAAGGCATGAAAACCCTGCAACAGTCCGGTATCGAGAAGCTATGCGAAGGCATCACCAGTTTAAAAGAGTTACAA
CGCGTACTGCACTTTAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.124

99.823

0.62
  pilB Vibrio parahaemolyticus RIMD 2210633

60.354

99.823

0.602
  pilB Vibrio campbellii strain DS40M4

59.717

100

0.597
  pilB Acinetobacter baumannii D1279779

49.82

98.233

0.489
  pilB Acinetobacter baylyi ADP1

49.545

96.996

0.481
  pilB Legionella pneumophila strain ERS1305867

49.907

95.23

0.475
  pilF Neisseria gonorrhoeae MS11

43.386

100

0.435
  pilB/pilB1 Synechocystis sp. PCC 6803

37.311

100

0.392
  pilF Thermus thermophilus HB27

36.508

100

0.366

Multiple sequence alignment