Detailed information NTG1170130

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: ACLV65_RS06235
• Genome accession: NZ_OZ217342
• Coordinates: 1223697..1224347 (-)
• Length: 216 a.a.
• NCBI ID: WP_411864804.1
• Uniprot ID: -
• Organism: Streptococcus mitis isolate S. mitis B22
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1218697..1229347

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV65_RS06215 (SMIB22_12190)	-	1219298..1220482 (-)	1185	WP_218773953.1	hypothetical protein	-
ACLV65_RS06220 (SMIB22_12200)	-	1220486..1221073 (-)	588	WP_176139747.1	ABC transporter ATP-binding protein	-
ACLV65_RS06225	-	1221106..1221294 (-)	189	WP_218773952.1	hypothetical protein	-
ACLV65_RS06230 (SMIB22_12210)	comEC/celB	1221473..1223713 (-)	2241	WP_411864803.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACLV65_RS06235 (SMIB22_12220)	comEA/celA/cilE	1223697..1224347 (-)	651	WP_411864804.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACLV65_RS06240 (SMIB22_12230)	-	1224414..1224983 (-)	570	WP_411864805.1	GNAT family N-acetyltransferase	-
ACLV65_RS06245 (SMIB22_12240)	-	1225090..1226094 (-)	1005	WP_049511760.1	PhoH family protein	-
ACLV65_RS06250 (SMIB22_12250)	-	1226179..1226394 (-)	216	WP_001232084.1	YozE family protein	-
ACLV65_RS06255 (SMIB22_12260)	cvfB	1226403..1227257 (-)	855	WP_411864806.1	RNA-binding virulence regulatory protein CvfB	-
ACLV65_RS06260 (SMIB22_12270)	frr	1227317..1227874 (-)	558	WP_001262225.1	ribosome recycling factor	-
ACLV65_RS06265 (SMIB22_12280)	pyrH	1227883..1228620 (-)	738	WP_000002996.1	UMP kinase	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23113.49 Da
• Isoelectric Point: 6.0684

>NTDB_id=1170130 ACLV65_RS06235 WP_411864804.1 1223697..1224347(-) (comEA/celA/cilE) [Streptococcus mitis isolate S. mitis B22]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQPPVKETNLQAEVAAVSKDSSTEKDVKKEEKEEPVEQDLITVDVKG
AVKAPGIYDLPVGSRVNDAVQKAGGLTEQADSKGLNLAQKVSDEALVYVPFKGEEVAGQQAGSGVPSSTSKDKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=1170130 ACLV65_RS06235 WP_411864804.1 1223697..1224347(-) (comEA/celA/cilE) [Streptococcus mitis isolate S. mitis B22]
ATGGAAGCAATTATCGAGAAAATTAAAGAGTATAAAATCATTGTTATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAGCCAGCTCCACAACCCCCTGTAAAAGAAACGAATTTACAGGCAGAAGTTGCAGCTGTTTCCAAGGATT
CATCGACCGAAAAGGATGTGAAGAAGGAAGAAAAGGAAGAACCGGTTGAACAAGATCTAATCACAGTAGATGTAAAAGGT
GCTGTTAAAGCACCTGGAATTTATGATTTGCCTGTAGGTAGTCGAGTCAATGACGCCGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGGGGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCATTTAAGGGAG
AAGAAGTAGCTGGTCAACAGGCAGGTTCTGGAGTACCTTCTTCAACAAGCAAGGACAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
GAAATTCAAGTCGGTTGATGAACTCAAGAAGGTCTCTGGCATTGGCGCTAAAACGATAGAAAAGTTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (152-214)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	93.519	100	0.935
comEA/celA/cilE	Streptococcus mitis NCTC 12261	93.056	100	0.931
comEA/celA/cilE	Streptococcus mitis SK321	93.056	100	0.931
comEA/celA/cilE	Streptococcus pneumoniae D39	90.741	100	0.907
comEA/celA/cilE	Streptococcus pneumoniae Rx1	90.741	100	0.907
comEA/celA/cilE	Streptococcus pneumoniae R6	90.741	100	0.907
comEA	Streptococcus thermophilus LMD-9	40.175	100	0.426
comEA	Lactococcus lactis subsp. cremoris KW2	40.444	100	0.421
comEA	Latilactobacillus sakei subsp. sakei 23K	34.335	100	0.37