Detailed information NTG1170129

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: ACLV65_RS06230
• Genome accession: NZ_OZ217342
• Coordinates: 1221473..1223713 (-)
• Length: 746 a.a.
• NCBI ID: WP_411864803.1
• Uniprot ID: -
• Organism: Streptococcus mitis isolate S. mitis B22
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1216473..1228713

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV65_RS06195 (SMIB22_12140)	-	1217378..1217758 (-)	381	WP_000157151.1	VOC family protein	-
ACLV65_RS06200 (SMIB22_12150)	rplT	1217816..1218175 (-)	360	WP_000124834.1	50S ribosomal protein L20	-
ACLV65_RS06205 (SMIB22_12160)	rpmI	1218227..1218427 (-)	201	WP_001125943.1	50S ribosomal protein L35	-
ACLV65_RS06210 (SMIB22_12170)	infC	1218460..1218990 (-)	531	WP_000848180.1	translation initiation factor IF-3	-
ACLV65_RS06215 (SMIB22_12190)	-	1219298..1220482 (-)	1185	WP_218773953.1	hypothetical protein	-
ACLV65_RS06220 (SMIB22_12200)	-	1220486..1221073 (-)	588	WP_176139747.1	ABC transporter ATP-binding protein	-
ACLV65_RS06225	-	1221106..1221294 (-)	189	WP_218773952.1	hypothetical protein	-
ACLV65_RS06230 (SMIB22_12210)	comEC/celB	1221473..1223713 (-)	2241	WP_411864803.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACLV65_RS06235 (SMIB22_12220)	comEA/celA/cilE	1223697..1224347 (-)	651	WP_411864804.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACLV65_RS06240 (SMIB22_12230)	-	1224414..1224983 (-)	570	WP_411864805.1	GNAT family N-acetyltransferase	-
ACLV65_RS06245 (SMIB22_12240)	-	1225090..1226094 (-)	1005	WP_049511760.1	PhoH family protein	-
ACLV65_RS06250 (SMIB22_12250)	-	1226179..1226394 (-)	216	WP_001232084.1	YozE family protein	-
ACLV65_RS06255 (SMIB22_12260)	cvfB	1226403..1227257 (-)	855	WP_411864806.1	RNA-binding virulence regulatory protein CvfB	-
ACLV65_RS06260 (SMIB22_12270)	frr	1227317..1227874 (-)	558	WP_001262225.1	ribosome recycling factor	-
ACLV65_RS06265 (SMIB22_12280)	pyrH	1227883..1228620 (-)	738	WP_000002996.1	UMP kinase	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84730.43 Da
• Isoelectric Point: 9.5261

>NTDB_id=1170129 ACLV65_RS06230 WP_411864803.1 1221473..1223713(-) (comEC/celB) [Streptococcus mitis isolate S. mitis B22]
MLQWIKTFPIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLICLFIQFPWKPAGKVLVICGVFGFWFLFQNWQQSQASQN
LADSVERVRILPDTIKVNGDSLSFRGKADGRIFQVYYKLQSEEEKEQFQALTALHDLELEGKLSEPERQRNFGGFDYQAY
LKTQGIYQTLNIKKIQSLQKVSSWDIGENLSSLRRKAVVWIKTHFPAPMRNYMTGILLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLILFII
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVARESLVISLGILPILSVYFAEFQPWSILLTFVFSFLFDLVFLPLLSI
LFALSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNAWLLILLLISLALVYDLRKNIKRIIVLSLLITGLFFLTK
HPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESDKKIEKWQEKAITSNAQRTLIPYLKSRGVAKIDQLILTNTDK
EHVGDLLEVTKAFHVGEILVSKGSLKQKEFVAELQETQIKVRSVAEGENVLIFGSQIEVLSPRKIGDGGHEDSLVLYGKL
LDKHFLFTGNLEEKGEKDLLKHYPDLKVDVLKASQHGSKKSLSSAFLEKLKPEFTLISVGKNNRTKLPHQETLIRLETIN
SKVYRTDQQGAIRFKGWNSWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=1170129 ACLV65_RS06230 WP_411864803.1 1221473..1223713(-) (comEC/celB) [Streptococcus mitis isolate S. mitis B22]
ATGTTACAGTGGATTAAGACTTTCCCTATTCCCCTAATCTATCTGAGTTTTCTATTACTCTGGCTTTATTACGCCATTTT
CTCAGCATCCTACCTTGCTTTGTTGGGCTTCGTTTTTCTGCTAATCTGTCTCTTTATCCAATTTCCTTGGAAACCTGCTG
GAAAAGTTCTAGTTATTTGCGGAGTCTTTGGCTTTTGGTTTCTGTTTCAAAATTGGCAACAGAGTCAAGCAAGTCAAAAT
TTGGCGGATTCTGTTGAAAGGGTACGGATTTTACCAGATACCATCAAAGTTAATGGTGATAGTCTATCCTTTCGTGGCAA
GGCTGATGGACGCATTTTTCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAACAATTTCAAGCTTTAACAGCCC
TTCATGATTTGGAACTAGAAGGAAAACTTTCAGAGCCAGAAAGGCAGAGAAATTTTGGTGGCTTTGACTATCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAAAAATCCAGTCACTTCAAAAGGTTAGCAGTTGGGATATAGG
TGAAAACCTGTCCAGTTTACGCCGTAAGGCTGTAGTTTGGATTAAGACGCATTTTCCAGCCCCTATGCGCAATTACATGA
CAGGGATTTTGCTAGGACATCTGGACACAGATTTTGAGGAGATGAATGAGCTTTATTCTAGTCTAGGAATTATCCACCTC
TTTGCCTTGTCTGGCATGCAGGTAGGTTTTTTCATGGATGGATTTAAGAAACTTCTCTTACGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACCTATCCCTTTTCCCTTATCTATGCAGGGCTGACAGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTACAAAAGTTACTGGCTCAACATGGTGTTAAGGGTTTGGATAATTTTGCCTTGACGGTCCTTATCCTCTTTATCATC
ATGCCCAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCTTGACCATGACTAGTAAAGAAGG
AGAGGGGCTCAAGGCTGTTGCTAGAGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTGTCCGTCTATTTTGCGG
AATTTCAACCTTGGTCCATCCTTTTGACCTTCGTCTTTTCCTTTCTATTTGACTTGGTCTTCTTACCGCTCTTGTCTATC
TTATTTGCCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAATTTTATTTTTGAATGGTTGGAGGGCATTATTCGCCTGGT
ATCGCAGGTGGCAAGTAGGCCTCTAGTCTTTGGACAACCCAATGCATGGCTTTTAATTTTATTGTTAATTTCCTTGGCTT
TAGTTTATGATTTGAGGAAAAACATTAAAAGGATAATAGTATTGAGTTTATTGATTACAGGTCTCTTTTTCCTTACCAAG
CATCCACTGGAAAATGAAATTACCATGCTGGATGTGGGGCAAGGCGAAAGTATTTTCCTAAGGGATGTAACTGGTAAGAC
TATTCTCATAGATGTGGGCGGTAAGGCAGAATCTGATAAGAAAATTGAAAAATGGCAAGAAAAGGCGATAACCAGCAATG
CCCAGCGAACCTTGATTCCTTATCTAAAAAGTAGAGGAGTAGCCAAAATTGACCAGCTGATTTTGACAAATACGGACAAG
GAGCATGTTGGAGATTTGTTGGAGGTGACCAAGGCTTTCCATGTAGGCGAAATTTTAGTGTCCAAAGGCAGTTTGAAACA
GAAGGAATTTGTGGCAGAACTACAAGAAACTCAAATCAAGGTGCGTAGTGTGGCAGAAGGGGAAAATGTGCTAATTTTCG
GAAGTCAGATAGAAGTACTATCTCCAAGGAAAATTGGAGATGGAGGTCATGAGGATTCCCTGGTTCTTTATGGGAAACTA
TTGGATAAGCACTTCCTCTTCACTGGAAATTTGGAGGAAAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTGGATGTTTTGAAAGCTAGCCAACATGGCTCTAAAAAATCATTAAGTTCAGCCTTTCTAGAAAAACTCAAACCAGAGT
TCACTCTCATTTCAGTTGGAAAGAATAATCGAACGAAACTCCCCCATCAGGAAACCTTGATACGACTGGAAACTATCAAT
AGCAAAGTTTACCGAACTGACCAGCAAGGAGCTATACGCTTTAAGGGGTGGAATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-696)

• Competence protein (Competence) (217-458)

• Domain of unknown function (DUF4131) (DUF4131) (31-177)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis SK321	94.504	100	0.945
comEC/celB	Streptococcus mitis NCTC 12261	92.349	99.866	0.922
comEC/celB	Streptococcus pneumoniae TIGR4	91.153	100	0.912
comEC/celB	Streptococcus pneumoniae D39	91.019	100	0.91
comEC/celB	Streptococcus pneumoniae Rx1	91.019	100	0.91
comEC/celB	Streptococcus pneumoniae R6	91.019	100	0.91
comEC	Lactococcus lactis subsp. cremoris KW2	44.804	99.33	0.445