Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFEL6_RS16430 Genome accession   NZ_OZ183475
Coordinates   2155837..2157528 (+) Length   563 a.a.
NCBI ID   WP_375321214.1    Uniprot ID   -
Organism   Aliivibrio logei isolate 622f83115ee5131b54a8b4d1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2150837..2162528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFEL6_RS16410 pdhR 2152427..2153197 (-) 771 WP_105055485.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACFEL6_RS16415 ampD 2153486..2154037 (-) 552 WP_375322823.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACFEL6_RS16420 nadC 2154162..2155049 (+) 888 WP_375321212.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFEL6_RS16425 pilA 2155390..2155836 (+) 447 WP_375321213.1 pilin Machinery gene
  ACFEL6_RS16430 pilB 2155837..2157528 (+) 1692 WP_375321214.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFEL6_RS16435 pilC 2157548..2158786 (+) 1239 WP_375321215.1 type II secretion system F family protein Machinery gene
  ACFEL6_RS16440 pilD 2158891..2159778 (+) 888 WP_375321216.1 prepilin peptidase Machinery gene
  ACFEL6_RS16445 coaE 2159775..2160398 (+) 624 WP_375321217.1 dephospho-CoA kinase -
  ACFEL6_RS16450 zapD 2160404..2161150 (+) 747 WP_105055492.1 cell division protein ZapD -
  ACFEL6_RS16455 yacG 2161158..2161379 (+) 222 WP_375321218.1 DNA gyrase inhibitor YacG -
  ACFEL6_RS16460 mutT 2161440..2161838 (-) 399 WP_375321219.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62283.02 Da        Isoelectric Point: 5.2860

>NTDB_id=1168049 ACFEL6_RS16430 WP_375321214.1 2155837..2157528(+) (pilB) [Aliivibrio logei isolate 622f83115ee5131b54a8b4d1]
MHTNLASILRQAELISATQESSIIESVQAETYTVPSALLAQGIFTPDELISHLAHIFGLDVINITQYDYADCCQKLGLRN
LITKYQALPISNDGCNIQLAVSDPSIAQVEDDFRFTTGKQITLVLADQGQLEAAVRRLYGRSISTSDSSHRKDITQDELA
SLVDVSLDEMTSTEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEDTYRVRFRCDGILVENSSPSSHLSRRLSARLK
IMAKLDIAERRLPQDGRIKLKLNSDISIDMRVSSLPTLWGEKIVLRLLDSSAPSLDIDKLGYSDKQKELYLNALKRPQGM
ILMTGPTGSGKTVSLYSGLNILNTTEKNISTAEDPVEINLNGVNQVQVAPKIGFTFAHALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLSLIIAQRLARRLCPHCKEVDPLSSETREEYSIDLL
STIFKARPEGCNECTHGYSGRIGIYEVMPFTRELSIAILKGATVRQIEQLAIKQGMDNLATSGIKRLEDGTTSLQELTRV
LYF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=1168049 ACFEL6_RS16430 WP_375321214.1 2155837..2157528(+) (pilB) [Aliivibrio logei isolate 622f83115ee5131b54a8b4d1]
ATGCACACCAACCTCGCCTCTATCTTGCGTCAAGCTGAGTTAATCAGTGCGACGCAAGAAAGCAGCATCATAGAGTCAGT
TCAAGCAGAAACGTACACTGTGCCTTCTGCTCTTTTAGCTCAAGGGATATTTACCCCTGATGAACTTATCAGCCATCTTG
CTCACATCTTTGGGTTAGATGTTATTAATATCACCCAATATGACTATGCTGATTGTTGTCAAAAGTTGGGCTTACGTAAT
CTCATCACTAAATATCAAGCGCTGCCTATTTCTAACGATGGTTGCAATATTCAACTGGCGGTAAGCGATCCAAGTATTGC
TCAAGTTGAAGATGATTTTCGCTTTACCACAGGAAAACAAATCACTCTCGTTTTAGCCGACCAAGGTCAGCTTGAAGCGG
CTGTTCGTCGTCTGTATGGACGTTCAATTTCAACTTCCGATTCATCACATCGTAAAGACATCACCCAAGATGAACTCGCT
TCATTGGTTGATGTCTCTCTTGATGAAATGACGTCTACCGAAGATCTCAGTCAAGATGATGCGCCAGTTACTCGCTTTAT
TAACCAGATTCTATTGGAGGCCGTCCGAAAAGGCGTTTCTGATATTCATTTTGAGCCTTATGAGGACACTTATCGAGTTC
GCTTTCGTTGTGATGGGATCTTAGTAGAGAACAGTTCACCTTCTTCACATTTAAGTCGTCGCCTTTCCGCTCGCTTAAAG
ATAATGGCTAAACTTGATATCGCAGAGCGTCGCTTACCACAAGATGGGCGAATTAAATTAAAACTGAACAGTGATATATC
GATAGATATGCGAGTTTCTTCCCTTCCCACCTTATGGGGAGAAAAAATCGTTTTACGTTTATTAGACAGCAGTGCCCCAA
GTTTAGATATTGATAAATTAGGCTACAGCGACAAACAAAAAGAGCTTTATCTTAACGCATTAAAACGCCCTCAAGGGATG
ATATTAATGACTGGCCCAACAGGCAGTGGTAAAACCGTCTCTCTCTATTCTGGTTTGAACATTTTAAATACCACAGAAAA
AAACATATCCACCGCTGAAGATCCTGTAGAAATAAATCTTAATGGGGTAAACCAAGTACAAGTTGCCCCTAAAATTGGTT
TTACTTTTGCTCATGCCTTACGTTCTTTTTTACGACAAGATCCAGACATCGTTATGGTCGGTGAAATTCGAGATCTCGAA
ACGGCAGAAATCGCAGTAAAAGCGGCGCAAACAGGGCACTTAGTACTTTCGACACTCCATACCAATTCAGCAGCAGAAAC
CGTAGTTCGCTTAGGTAATATGGGGATAGAAAACTTCAACCTTGCTTCTTCCCTTAGTTTAATTATCGCCCAACGCTTAG
CGAGGAGACTTTGCCCTCACTGTAAAGAAGTTGACCCTCTGAGTTCAGAAACACGAGAAGAATATAGTATCGATTTATTA
AGTACTATTTTTAAAGCTCGACCTGAGGGCTGTAATGAATGTACTCATGGTTACTCTGGCCGTATCGGTATTTACGAAGT
TATGCCCTTTACGCGTGAATTATCAATCGCCATCCTTAAAGGAGCGACAGTAAGACAAATTGAACAACTCGCCATCAAAC
AAGGTATGGACAACCTAGCTACATCCGGCATTAAACGACTTGAAGATGGCACAACGAGCTTACAAGAACTCACTCGTGTT
CTTTATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

68.561

100

0.686
  pilB Vibrio parahaemolyticus RIMD 2210633

67.851

100

0.679
  pilB Vibrio campbellii strain DS40M4

66.785

100

0.668
  pilB Acinetobacter baumannii D1279779

47.772

99.645

0.476
  pilB Legionella pneumophila strain ERS1305867

46.524

99.645

0.464
  pilB Acinetobacter baylyi ADP1

49.608

90.586

0.449
  pilF Neisseria gonorrhoeae MS11

43.54

100

0.437
  pilB/pilB1 Synechocystis sp. PCC 6803

35.537

100

0.382

Multiple sequence alignment