Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RG43_RS21010 Genome accession   NZ_CP010157
Coordinates   3968155..3969540 (+) Length   461 a.a.
NCBI ID   WP_073507042.1    Uniprot ID   -
Organism   Escherichia coli strain D10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3963155..3974540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG43_RS20980 (RG43_19755) aroP 3963706..3965076 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  RG43_RS20985 (RG43_19760) ampE 3965119..3965973 (-) 855 WP_000171975.1 beta-lactamase regulator AmpE -
  RG43_RS20990 (RG43_19765) ampD 3965970..3966521 (-) 552 WP_000923730.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG43_RS20995 (RG43_19770) nadC 3966609..3967502 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG43_RS21005 (RG43_19780) pilA 3967705..3968145 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  RG43_RS21010 (RG43_19785) pilB 3968155..3969540 (+) 1386 WP_073507042.1 type II secretion system protein GspE Machinery gene
  RG43_RS21015 (RG43_19790) hofC 3969530..3970732 (+) 1203 WP_000157293.1 protein transport protein HofC -
  RG43_RS21020 (RG43_19795) guaC 3970767..3971810 (-) 1044 WP_001217338.1 GMP reductase -
  RG43_RS29505 - 3971966..3972010 (-) 45 WP_120795372.1 protein YacM -
  RG43_RS21025 (RG43_19800) coaE 3972035..3972655 (+) 621 WP_001269512.1 dephospho-CoA kinase -
  RG43_RS21030 (RG43_19805) zapD 3972655..3973398 (+) 744 WP_001194743.1 cell division protein ZapD -
  RG43_RS21035 (RG43_19810) yacG 3973408..3973605 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  RG43_RS21045 (RG43_19820) mutT 3973807..3974205 (-) 399 WP_000736051.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50711.44 Da        Isoelectric Point: 6.5405

>NTDB_id=116527 RG43_RS21010 WP_073507042.1 3968155..3969540(+) (pilB) [Escherichia coli strain D10]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRLQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPFIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMQHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=116527 RG43_RS21010 WP_073507042.1 3968155..3969540(+) (pilB) [Escherichia coli strain D10]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACCCAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGTGTATTGCATCCTTTACCGGATGTTTTACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGCCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTGGGGATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGTCTTACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAATTCC
ACCACTGAAACACTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGCCGCCTGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGTTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGAATGCAGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.328

100

0.419
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Haemophilus influenzae 86-028NP

39.915

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.923

84.599

0.397
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

84.599

0.395
  pilB Acinetobacter baylyi ADP1

45.22

83.948

0.38
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Acinetobacter baumannii D1279779

44.072

84.165

0.371
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilF Thermus thermophilus HB27

41.439

87.419

0.362

Multiple sequence alignment