Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RG43_RS21005 Genome accession   NZ_CP010157
Coordinates   3967705..3968145 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain D10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3962705..3973145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG43_RS20980 (RG43_19755) aroP 3963706..3965076 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  RG43_RS20985 (RG43_19760) ampE 3965119..3965973 (-) 855 WP_000171975.1 beta-lactamase regulator AmpE -
  RG43_RS20990 (RG43_19765) ampD 3965970..3966521 (-) 552 WP_000923730.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG43_RS20995 (RG43_19770) nadC 3966609..3967502 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG43_RS21005 (RG43_19780) pilA 3967705..3968145 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  RG43_RS21010 (RG43_19785) pilB 3968155..3969540 (+) 1386 WP_073507042.1 type II secretion system protein GspE Machinery gene
  RG43_RS21015 (RG43_19790) hofC 3969530..3970732 (+) 1203 WP_000157293.1 protein transport protein HofC -
  RG43_RS21020 (RG43_19795) guaC 3970767..3971810 (-) 1044 WP_001217338.1 GMP reductase -
  RG43_RS29505 - 3971966..3972010 (-) 45 WP_120795372.1 protein YacM -
  RG43_RS21025 (RG43_19800) coaE 3972035..3972655 (+) 621 WP_001269512.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=116526 RG43_RS21005 WP_000360904.1 3967705..3968145(+) (pilA) [Escherichia coli strain D10]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=116526 RG43_RS21005 WP_000360904.1 3967705..3968145(+) (pilA) [Escherichia coli strain D10]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment