Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEN42_RS02790 Genome accession   NZ_OZ032133
Coordinates   580628..581845 (-) Length   405 a.a.
NCBI ID   WP_371880575.1    Uniprot ID   -
Organism   Caballeronia sp. S22 isolate ena-SAMPLE-TAB-09-04-2024-15:28:32:345-2572     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 575628..586845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN42_RS02760 - 576228..576659 (+) 432 WP_371880571.1 NUDIX domain-containing protein -
  ACEN42_RS02765 yacG 576673..576864 (-) 192 WP_187606093.1 DNA gyrase inhibitor YacG -
  ACEN42_RS02770 zapD 576935..577690 (-) 756 WP_035510226.1 cell division protein ZapD -
  ACEN42_RS02775 coaE 577861..578466 (-) 606 WP_371880572.1 dephospho-CoA kinase -
  ACEN42_RS02780 - 578483..579394 (-) 912 WP_371880573.1 A24 family peptidase -
  ACEN42_RS02785 - 579426..580631 (-) 1206 WP_371880574.1 type II secretion system F family protein -
  ACEN42_RS02790 pilB 580628..581845 (-) 1218 WP_371880575.1 GspE/PulE family protein Machinery gene
  ACEN42_RS02795 - 581920..583209 (-) 1290 WP_035511302.1 HlyC/CorC family transporter -
  ACEN42_RS02805 - 583470..584462 (-) 993 WP_238212815.1 polyprenyl synthetase family protein -
  ACEN42_RS02810 rplU 584719..585030 (+) 312 WP_035511298.1 50S ribosomal protein L21 -
  ACEN42_RS02815 rpmA 585074..585334 (+) 261 WP_008342952.1 50S ribosomal protein L27 -
  ACEN42_RS02820 cgtA 585500..586585 (+) 1086 WP_035511296.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43431.77 Da        Isoelectric Point: 7.2505

>NTDB_id=1163304 ACEN42_RS02790 WP_371880575.1 580628..581845(-) (pilB) [Caballeronia sp. S22 isolate ena-SAMPLE-TAB-09-04-2024-15:28:32:345-2572]
MTAPSRDAFDTFARPARAAESARDNASDQETPAVSLLAETLREAAERGASDIHVEPSEHDWRIRLRVDGALHVLGRPPPH
LRDAFLTRIKVLARMDIAERRVPQDGRLRLKLPGGRIGDYRVNSLPTLFGEKLVLRRLDALPPDLSLAALGLDDVQSGAV
ESAIRAPHGLILVTGPTGSGKTLSLYCFLQMLNAEARNVCSVEDPVEIQLAGINQVSVREKAGLTFAVALRAFMRQDPDV
IMVGEIRDAETADVAVGAAQTGHLVLSTLHTNDAPAAIARLIDIGVAPYNLASALRLVTAQRLVRKLCEICRAPSALPLA
GGFHPFEARGCAACHGIGYRGRVGVHQVMPVSDELRELIVSRAATHAISRAAQARGMPSLRDAAFARVRDGVTSVAEAAE
ATEIE

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1163304 ACEN42_RS02790 WP_371880575.1 580628..581845(-) (pilB) [Caballeronia sp. S22 isolate ena-SAMPLE-TAB-09-04-2024-15:28:32:345-2572]
ATGACTGCCCCTTCCCGCGACGCTTTCGACACCTTCGCACGCCCGGCCCGTGCGGCCGAATCCGCACGCGACAACGCATC
CGATCAAGAAACGCCCGCCGTCTCGCTGCTCGCCGAAACCTTGCGCGAGGCCGCCGAGCGCGGCGCATCCGATATCCACG
TCGAGCCATCGGAGCACGACTGGCGCATCCGGCTGCGCGTCGATGGCGCGCTGCATGTGTTGGGGCGTCCGCCGCCGCAC
TTGCGCGACGCGTTCCTCACGCGCATCAAGGTGCTGGCGCGCATGGACATCGCCGAGCGGCGCGTGCCGCAGGACGGCCG
GCTGCGTCTGAAACTTCCGGGCGGGCGCATCGGCGACTATCGCGTCAATTCGCTGCCGACGCTCTTCGGCGAAAAGCTCG
TGCTGCGGCGGCTCGACGCGTTGCCGCCCGATCTGTCGCTCGCCGCGCTCGGTCTCGACGATGTGCAAAGCGGCGCGGTC
GAATCGGCGATTCGCGCGCCGCACGGTCTCATCCTCGTGACCGGACCGACCGGCAGCGGCAAGACTCTTTCGCTCTATTG
CTTCCTTCAGATGCTCAATGCCGAAGCGCGCAACGTATGCTCCGTCGAAGATCCCGTCGAGATTCAGCTCGCGGGCATCA
ATCAGGTGAGCGTGCGCGAAAAAGCGGGGCTGACGTTCGCCGTGGCGCTGCGCGCGTTCATGCGACAGGATCCGGACGTC
ATCATGGTCGGCGAAATTCGCGACGCTGAAACCGCCGATGTCGCGGTGGGGGCGGCGCAGACGGGACACCTCGTGCTCTC
GACCTTGCACACCAACGACGCTCCGGCCGCGATCGCGCGGCTCATCGATATCGGCGTGGCGCCCTACAACCTCGCGTCCG
CGCTGAGGCTCGTAACCGCGCAACGGCTCGTGCGCAAGCTGTGCGAGATTTGCCGCGCGCCATCGGCGTTGCCGCTCGCG
GGCGGGTTTCATCCATTCGAAGCGCGCGGCTGTGCGGCGTGTCATGGCATCGGTTATCGCGGGCGCGTCGGCGTGCATCA
GGTGATGCCGGTATCCGATGAACTACGCGAACTGATCGTATCGCGCGCGGCAACGCATGCAATCTCGCGAGCGGCGCAGG
CGCGTGGCATGCCCTCATTGCGCGATGCAGCCTTCGCCCGCGTGCGCGACGGCGTGACGAGCGTCGCCGAGGCAGCCGAA
GCAACGGAGATCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(40-306)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

48.883

99.506

0.486
  pilB Acinetobacter baumannii D1279779

47.87

98.519

0.472
  pilF Neisseria gonorrhoeae MS11

48.101

97.531

0.469
  pilB Vibrio campbellii strain DS40M4

48.303

94.568

0.457
  pilB Acinetobacter baylyi ADP1

48.052

95.062

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

48.042

94.568

0.454
  pilB Legionella pneumophila strain ERS1305867

46.231

98.272

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

43.552

100

0.442
  pilF Thermus thermophilus HB27

41

98.765

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.451

95.309

0.395
  pilB Glaesserella parasuis strain SC1401

41.689

93.58

0.39
  pilB Haemophilus influenzae 86-028NP

42.133

92.593

0.39
  pilB Haemophilus influenzae Rd KW20

46.177

80.741

0.373

Multiple sequence alignment