Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BH81_RS21475 Genome accession   NZ_CP007039
Coordinates   5057097..5058314 (+) Length   405 a.a.
NCBI ID   WP_025261831.1    Uniprot ID   -
Organism   Pseudomonas cichorii JBC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5052097..5063314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BH81_RS21455 (PCH70_43290) - 5052752..5053300 (+) 549 WP_025261828.1 DUF1523 family protein -
  BH81_RS21460 (PCH70_43300) - 5053307..5054698 (-) 1392 WP_236250088.1 O-antigen ligase -
  BH81_RS26380 - 5054782..5055171 (-) 390 WP_038400031.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BH81_RS21470 (PCH70_43310) pilB 5055397..5057094 (+) 1698 WP_025261830.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BH81_RS21475 (PCH70_43320) pilC 5057097..5058314 (+) 1218 WP_025261831.1 type II secretion system F family protein Machinery gene
  BH81_RS21480 (PCH70_43330) pilD 5058316..5059188 (+) 873 WP_025261832.1 A24 family peptidase Machinery gene
  BH81_RS21485 (PCH70_43340) coaE 5059185..5059808 (+) 624 WP_025261833.1 dephospho-CoA kinase -
  BH81_RS21490 (PCH70_43350) yacG 5059805..5060014 (+) 210 WP_025261834.1 DNA gyrase inhibitor YacG -
  BH81_RS21495 (PCH70_43360) - 5060008..5060475 (-) 468 WP_025261835.1 hypothetical protein -
  BH81_RS21500 (PCH70_43370) - 5060593..5062476 (-) 1884 WP_025261836.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44232.16 Da        Isoelectric Point: 10.0128

>NTDB_id=116201 BH81_RS21475 WP_025261831.1 5057097..5058314(+) (pilC) [Pseudomonas cichorii JBC1]
MASKAVKVTVYTWEGLDKKGSKMTGELSGHNPALIKAQLRKQGINPTKVRKKSVSIFGNGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVDFLKQEVSAGNSLATALRQKPEYFDDLYCNLVDAGEQAGALESLLDRVATYKEKT
EALKSKIKKAMTYPIAVMVVAVIVSGILLIKVVPQFQSVFAGFNAQLPAFTLMVIGLSEIVQNWWLAIVGLFIVGFFMFK
RAYKQSQNFRDRIDRLLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVNKIKQDVSTGM
QLNFSMRSVNVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVIVGGLVLAMYLPIFK
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=116201 BH81_RS21475 WP_025261831.1 5057097..5058314(+) (pilC) [Pseudomonas cichorii JBC1]
ATGGCTAGCAAAGCAGTCAAAGTCACTGTTTACACCTGGGAAGGCCTGGACAAGAAAGGCTCGAAGATGACAGGTGAGTT
GAGCGGCCATAACCCGGCACTCATCAAGGCACAGTTGCGCAAGCAGGGCATCAATCCCACAAAAGTGCGTAAAAAGTCCG
TCTCCATATTCGGCAACGGCAAGAAGATCAAGCCGCTGGATATCGCCTTTTTCTCAAGACAAATGGCGACGATGATGAAA
GCAGGCGTTCCACTGCTGCAATCTTTCGACATCATCAGCGAGGGTGCCGAAAACCCGAACATGCGGGCGCTGGTGGACTT
CCTGAAGCAGGAGGTATCGGCGGGCAACAGCCTGGCAACGGCCCTGCGACAAAAGCCTGAGTACTTTGACGACCTGTATT
GCAACCTGGTGGATGCCGGCGAGCAGGCGGGTGCCCTTGAAAGCCTGCTGGATCGGGTTGCCACCTATAAGGAAAAGACC
GAGGCACTCAAGTCCAAGATCAAGAAGGCCATGACCTACCCGATTGCTGTCATGGTTGTAGCGGTGATTGTTTCGGGTAT
TTTGCTGATCAAGGTCGTGCCGCAATTTCAGTCGGTATTTGCAGGTTTTAATGCTCAATTACCCGCCTTTACCTTGATGG
TCATCGGCCTATCAGAAATCGTGCAGAATTGGTGGCTGGCCATCGTCGGTCTTTTTATTGTCGGCTTTTTCATGTTCAAA
CGAGCCTACAAGCAATCCCAGAACTTTCGTGACCGTATCGACAGACTCCTGTTGAAAGTCCCGATCATTGGGCCGTTGAT
TTTCAAATCATCTGTAGCTCGTTATGCACGAACCCTTGCAACAACCTTCGCAGCAGGCGTTCCGCTGGTGGAGGCACTTG
ACTCGGTAGCAGGTGCAACCGGGAATGTGGTGTTCAAGAATGCGGTCAACAAGATCAAGCAAGACGTTTCAACCGGTATG
CAACTGAACTTCTCAATGCGTTCAGTGAATGTATTCCCGAGCCTGGCCATTCAAATGACTGCGATTGGTGAAGAGTCCGG
TGCACTGGACTCCATGCTCGACAAGGTTGCGACTTACTACGAGGACGAAGTCGATAATATGGTCGACAGCCTTACCAGCC
TGATGGAGCCCATGATCATGGCGGTACTCGGCGTTATCGTGGGAGGCCTGGTGCTGGCCATGTACTTGCCTATCTTCAAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.815

100

0.748
  pilC Acinetobacter baylyi ADP1

59.124

100

0.6
  pilC Acinetobacter baumannii D1279779

58.663

99.753

0.585
  pilC Legionella pneumophila strain ERS1305867

53.283

97.778

0.521
  pilG Neisseria gonorrhoeae MS11

42.963

100

0.43
  pilG Neisseria meningitidis 44/76-A

42.716

100

0.427
  pilC Vibrio campbellii strain DS40M4

41.22

100

0.417
  pilC Vibrio cholerae strain A1552

40.988

100

0.41
  pilC Thermus thermophilus HB27

39.152

99.012

0.388

Multiple sequence alignment