Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLMBU_RS25635 Genome accession   NZ_OY782519
Coordinates   5493130..5494338 (+) Length   402 a.a.
NCBI ID   WP_321359333.1    Uniprot ID   -
Organism   MAG: Pseudomonas extremaustralis isolate 6328a48c-d287-402b-9a22-4be284e47c5f     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5488130..5499338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLMBU_RS25610 (U3A02_RS25565) nadC 5489618..5490466 (+) 849 WP_010565569.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLMBU_RS25615 (U3A02_RS25570) - 5490514..5490819 (-) 306 WP_321359330.1 DUF6388 family protein -
  ACLMBU_RS25625 (U3A02_RS25580) - 5491077..5492477 (-) 1401 WP_321359332.1 O-antigen ligase family protein -
  ACLMBU_RS25630 (U3A02_RS25585) - 5492513..5492926 (-) 414 WP_010565572.1 pilin -
  ACLMBU_RS25635 (U3A02_RS25590) pilC 5493130..5494338 (+) 1209 WP_321359333.1 type II secretion system F family protein Machinery gene
  ACLMBU_RS25640 (U3A02_RS25595) pilD 5494335..5495201 (+) 867 WP_321359336.1 A24 family peptidase Machinery gene
  ACLMBU_RS25645 (U3A02_RS25600) coaE 5495198..5495821 (+) 624 WP_078835105.1 dephospho-CoA kinase -
  ACLMBU_RS25650 (U3A02_RS25605) yacG 5495818..5496018 (+) 201 WP_010565576.1 DNA gyrase inhibitor YacG -
  ACLMBU_RS25655 (U3A02_RS25610) - 5496015..5496230 (-) 216 WP_003171680.1 hypothetical protein -
  ACLMBU_RS25660 (U3A02_RS25615) - 5496306..5496995 (-) 690 WP_321359371.1 energy-coupling factor ABC transporter permease -
  ACLMBU_RS25665 (U3A02_RS25620) - 5496992..5497459 (-) 468 WP_010565578.1 hypothetical protein -
  ACLMBU_RS25670 (U3A02_RS25625) - 5497559..5498188 (+) 630 WP_010565579.1 DUF1780 domain-containing protein -
  ACLMBU_RS25675 (U3A02_RS25630) - 5498243..5498416 (+) 174 WP_010565580.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43385.65 Da        Isoelectric Point: 9.8101

>NTDB_id=1161915 ACLMBU_RS25635 WP_321359333.1 5493130..5494338(+) (pilC) [MAG: Pseudomonas extremaustralis isolate 6328a48c-d287-402b-9a22-4be284e47c5f]
MNNPSTLYAWEGINRKGHRVCGQATGHTPAMVKAQLRRQGISPGRVHKKSPLLPSLAPRIKPADITLFTRQLATLLKAGI
ALLQAFDIISEGFDNQSVRELVQGLKREIAAGTSLTDALRQHPRYFDELYCNLVAAGEQAGTLETLLERVAIHREKSEQL
KARIKKAMTYPLAVLLIASLVTGVLLIQVVPQFQTLFAGVGGNLPGFTLQVIALSEFMQQTWWVVALGLGAGAVGLRHAY
RASVDFRHWLDATLLKAPLAGKLLQKSAVARYARTLSTTFAAGVPLLQALGCVAGAAGSGPFRQAIEHMRHEVSTGMQLN
QSMAASGLFPGMAIQMTAIGEESGTLDRMLEKVASHYEADVDNQVDNLTQLMEPLMMVVLGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=1161915 ACLMBU_RS25635 WP_321359333.1 5493130..5494338(+) (pilC) [MAG: Pseudomonas extremaustralis isolate 6328a48c-d287-402b-9a22-4be284e47c5f]
ATGAACAACCCATCGACGCTTTACGCCTGGGAAGGCATCAACCGCAAAGGACACCGGGTGTGCGGGCAAGCCACCGGCCA
CACCCCCGCCATGGTCAAGGCGCAACTGCGTCGGCAAGGCATCAGCCCAGGCCGTGTACACAAAAAATCCCCTTTGTTGC
CAAGCCTGGCCCCACGGATAAAACCGGCGGACATCACCCTGTTCACTCGCCAACTGGCCACCCTGCTGAAAGCGGGGATC
GCGCTGCTGCAAGCCTTCGACATCATCAGCGAAGGCTTCGACAACCAATCGGTGCGTGAACTGGTGCAGGGTTTGAAGCG
GGAGATCGCCGCCGGCACCAGCCTGACCGATGCCCTGCGCCAACATCCGCGTTATTTCGACGAACTGTATTGCAACCTGG
TGGCCGCCGGCGAACAAGCGGGGACCCTTGAGACCCTGCTGGAGCGCGTCGCGATCCACCGGGAAAAGAGTGAGCAGCTT
AAAGCCAGAATCAAAAAAGCCATGACCTACCCGCTCGCGGTCCTGCTGATCGCAAGTCTTGTCACGGGTGTTCTGTTGAT
TCAAGTGGTGCCGCAATTCCAGACCCTGTTCGCCGGAGTCGGCGGCAATCTGCCCGGTTTTACCTTGCAGGTCATCGCCC
TGTCCGAGTTCATGCAGCAAACCTGGTGGGTGGTGGCGCTGGGCCTGGGCGCGGGGGCCGTGGGCTTGCGTCATGCCTAT
CGCGCGTCCGTGGATTTTCGTCACTGGCTGGACGCCACTTTGTTGAAAGCGCCCCTGGCAGGCAAACTGCTGCAAAAATC
GGCAGTGGCCCGCTATGCCCGAACCCTCTCCACCACCTTTGCCGCCGGTGTTCCGCTGCTACAGGCGCTGGGTTGCGTGG
CCGGTGCGGCCGGCTCAGGTCCGTTCAGACAGGCAATCGAGCATATGCGACACGAGGTATCCACGGGCATGCAGTTGAAT
CAATCCATGGCTGCCAGTGGCCTGTTTCCCGGCATGGCAATCCAGATGACGGCGATCGGCGAAGAGTCCGGCACGCTGGA
CCGCATGCTGGAAAAAGTCGCCAGCCATTACGAAGCCGATGTGGATAACCAGGTCGACAACCTCACGCAGTTGATGGAAC
CACTGATGATGGTAGTGCTGGGCGGGATTGTCGGGGCACTGGTCATTGCCATGTACTTGCCGGTGTTCCAGCTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.091

98.507

0.582
  pilC Acinetobacter baylyi ADP1

49.747

98.507

0.49
  pilC Acinetobacter baumannii D1279779

49.367

98.259

0.485
  pilC Legionella pneumophila strain ERS1305867

48.507

100

0.485
  pilC Vibrio cholerae strain A1552

40.25

99.502

0.4
  pilG Neisseria gonorrhoeae MS11

39.848

98.01

0.391
  pilC Vibrio campbellii strain DS40M4

38.889

98.507

0.383
  pilG Neisseria meningitidis 44/76-A

39.086

98.01

0.383

Multiple sequence alignment