Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QEN43_RS00735 Genome accession   NZ_OX458333
Coordinates   162938..164653 (-) Length   571 a.a.
NCBI ID   WP_026610411.1    Uniprot ID   -
Organism   Methylocaldum szegediense isolate Msz(Nor)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 157938..169653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEN43_RS00705 (MSZNOR_0175) - 158610..158894 (-) 285 WP_026610405.1 FmdB family zinc ribbon protein -
  QEN43_RS00710 (MSZNOR_0176) - 159076..159294 (-) 219 WP_026610406.1 DNA gyrase inhibitor YacG -
  QEN43_RS00715 (MSZNOR_0177) zapD 159304..160098 (-) 795 WP_235726606.1 cell division protein ZapD -
  QEN43_RS00720 (MSZNOR_0178) coaE 160215..160814 (-) 600 WP_026610408.1 dephospho-CoA kinase -
  QEN43_RS00725 (MSZNOR_0179) pilD 160820..161701 (-) 882 WP_036268865.1 A24 family peptidase Machinery gene
  QEN43_RS00730 (MSZNOR_0180) pilC 161718..162932 (-) 1215 WP_026610410.1 type II secretion system F family protein Machinery gene
  QEN43_RS00735 (MSZNOR_0181) pilB 162938..164653 (-) 1716 WP_026610411.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QEN43_RS00740 (MSZNOR_0182) - 165173..168016 (-) 2844 WP_026610412.1 valine--tRNA ligase -
  QEN43_RS00745 (MSZNOR_0183) - 168106..168546 (-) 441 WP_026610413.1 DNA polymerase III subunit chi -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63254.89 Da        Isoelectric Point: 6.1226

>NTDB_id=1158458 QEN43_RS00735 WP_026610411.1 162938..164653(-) (pilB) [Methylocaldum szegediense isolate Msz(Nor)]
MAITATKLQLSGLAKSLVKEGLLTESDAQLHLEDALQKKTHFISYLVSNNILDSLSIANVASKEYGIPLFDLDAMSPDMM
PIKAVSEKLIRQHHVLPLFKRGNRLYLGVADPTNLQALDEIKFHTRSSTECVIVEEDKLTRMIDQALEAAEASFKDLVDA
DLDNLRITGGDEIDQQSESETPDIDDAPIVRLVNKFLLDAIKKGASDIHFEPYEKTFRVRFRHDGILHEVTSLPVNLAGR
VAARLKVMSRMDIAERRIPQDGRIKMALSRTRAVDFRVNTCPTLFGEKIVLRILDPTAAQIGIEKLGFEPEQQQNFLHAI
NKPYGMILVTGPTGSGKTVSLYTALNILNTADRNISTAEDPVEITVPGINQVNVNVKTGLTFAEALRAFLRQDPDVIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIASSVLLIMAQRLARRLCEHCKKEENLPPEVLLQ
AGFREEELGRFTVFGPVGCDKCVKGYKGRVGIYQVMPISESINRIILEGGNVLQLSEQAKREGVADLRESGLRKVKAGIT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1158458 QEN43_RS00735 WP_026610411.1 162938..164653(-) (pilB) [Methylocaldum szegediense isolate Msz(Nor)]
ATGGCGATCACGGCGACGAAATTGCAACTTAGCGGACTGGCCAAAAGCCTGGTCAAAGAAGGATTGTTGACAGAGTCCGA
CGCGCAGCTTCATCTGGAGGACGCTCTCCAAAAAAAAACGCATTTCATAAGTTACCTGGTCAGTAATAACATTCTCGACA
GCCTGTCCATCGCCAATGTCGCGTCCAAAGAGTACGGAATACCTCTCTTCGATCTGGACGCGATGTCGCCCGACATGATG
CCGATCAAGGCCGTCAGCGAGAAGCTGATTCGCCAACACCACGTCCTACCGCTCTTCAAGCGCGGAAACCGGCTGTATCT
GGGTGTCGCCGATCCCACCAATCTTCAGGCTCTGGACGAAATCAAATTTCACACCCGGTCCAGCACCGAATGTGTCATCG
TCGAGGAAGACAAACTGACACGGATGATCGACCAGGCGCTGGAAGCGGCCGAAGCCTCGTTCAAAGACTTGGTGGACGCC
GACCTGGACAATCTCCGTATTACTGGCGGCGACGAAATCGATCAACAGTCCGAAAGTGAAACCCCCGACATCGACGATGC
CCCGATCGTCCGACTCGTCAACAAGTTTCTCCTAGACGCCATCAAGAAAGGCGCTTCCGATATTCACTTCGAGCCCTACG
AGAAAACCTTCCGCGTCCGTTTTCGTCACGACGGCATACTCCACGAGGTCACGAGCCTGCCGGTTAACCTCGCCGGGCGG
GTTGCGGCGCGACTTAAGGTGATGTCGCGCATGGACATCGCCGAACGGCGCATACCCCAGGACGGTCGCATCAAGATGGC
GTTGTCCAGGACTCGCGCCGTCGATTTCCGCGTCAATACTTGCCCTACGCTATTCGGCGAAAAAATCGTACTCCGTATCC
TGGATCCGACCGCCGCCCAGATCGGCATCGAAAAACTCGGCTTCGAACCGGAGCAACAACAGAACTTCCTGCACGCCATC
AATAAACCCTATGGAATGATCCTGGTCACGGGACCTACAGGCAGCGGTAAAACGGTAAGTCTCTACACCGCACTCAATAT
CCTGAATACAGCTGACCGCAACATCTCGACGGCGGAAGATCCGGTTGAAATCACGGTGCCCGGGATCAATCAGGTCAATG
TCAACGTCAAGACAGGCTTGACCTTTGCCGAGGCACTAAGGGCATTTCTGCGCCAGGACCCGGACGTCATCATGGTGGGC
GAAATTCGCGATCTGGAAACCGCCGAAATCGCGGTCAAAGCCGCGCAGACCGGCCATCTCGTGCTTTCCACCCTGCATAC
CAACGATGCGCCGCAAACCCTGAACCGCTTGATGCAAATGGGCATCCCCGCGTTCAACATCGCGTCTTCCGTGCTCTTGA
TTATGGCGCAGAGACTCGCCCGAAGGCTTTGCGAACACTGCAAAAAAGAAGAAAACCTCCCTCCGGAAGTTCTGTTGCAG
GCCGGTTTCCGAGAAGAAGAACTAGGCAGGTTCACGGTCTTCGGACCGGTCGGCTGCGACAAATGTGTGAAGGGGTACAA
GGGTCGGGTTGGCATTTATCAGGTTATGCCCATTTCCGAGTCCATCAACCGCATCATTCTCGAAGGCGGTAATGTCCTTC
AGCTTAGCGAGCAAGCGAAGCGAGAAGGGGTAGCGGACCTCAGGGAATCCGGCCTTCGGAAAGTCAAGGCCGGCATCACC
AGCTTGGAAGAAATCGACCGCGTCACTCGAGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.208

100

0.557
  pilB Acinetobacter baylyi ADP1

53.46

100

0.541
  pilB Acinetobacter baumannii D1279779

53.578

100

0.538
  pilB Vibrio parahaemolyticus RIMD 2210633

48.325

99.299

0.48
  pilB Vibrio campbellii strain DS40M4

47.78

98.599

0.471
  pilF Neisseria gonorrhoeae MS11

47.34

98.774

0.468
  pilB Vibrio cholerae strain A1552

51.772

88.967

0.461
  pilF Thermus thermophilus HB27

39.437

99.475

0.392
  pilB/pilB1 Synechocystis sp. PCC 6803

39.748

97.373

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.266

95.447

0.375

Multiple sequence alignment