Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QEN43_RS00725 Genome accession   NZ_OX458333
Coordinates   160820..161701 (-) Length   293 a.a.
NCBI ID   WP_036268865.1    Uniprot ID   -
Organism   Methylocaldum szegediense isolate Msz(Nor)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 155820..166701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEN43_RS00700 (MSZNOR_0174) aspS 156758..158542 (-) 1785 WP_026610404.1 aspartate--tRNA ligase -
  QEN43_RS00705 (MSZNOR_0175) - 158610..158894 (-) 285 WP_026610405.1 FmdB family zinc ribbon protein -
  QEN43_RS00710 (MSZNOR_0176) - 159076..159294 (-) 219 WP_026610406.1 DNA gyrase inhibitor YacG -
  QEN43_RS00715 (MSZNOR_0177) zapD 159304..160098 (-) 795 WP_235726606.1 cell division protein ZapD -
  QEN43_RS00720 (MSZNOR_0178) coaE 160215..160814 (-) 600 WP_026610408.1 dephospho-CoA kinase -
  QEN43_RS00725 (MSZNOR_0179) pilD 160820..161701 (-) 882 WP_036268865.1 A24 family peptidase Machinery gene
  QEN43_RS00730 (MSZNOR_0180) pilC 161718..162932 (-) 1215 WP_026610410.1 type II secretion system F family protein Machinery gene
  QEN43_RS00735 (MSZNOR_0181) pilB 162938..164653 (-) 1716 WP_026610411.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 293 a.a.        Molecular weight: 32737.76 Da        Isoelectric Point: 7.9454

>NTDB_id=1158456 QEN43_RS00725 WP_036268865.1 160820..161701(-) (pilD) [Methylocaldum szegediense isolate Msz(Nor)]
MDSFVRLLAENPALFLTVVGVLGLMVGSFLNVVIHRLPIMLERNWRRECRAFLDLEPEPEPDQPYNLLVPGSRCPRCDRP
IRAWENIPIVSYLLLKRRCKGCRTPIPFRYPAIEALTALISIAVAWHFGFTWQTASALILSWSLICLSFIDFDRQLLPDA
ITLPVLWTGLFLNVFNIFTDSASSILGAIFGYLSLWLVYHVFRLATGKEGMGYGDFKLLAMLGAWLGWSLLPVVVLLSSL
VGAVIGTTMVAVFRRDRSIPIPFGPYLAIAGWIALMWGDDINAAYLNFAGISG

Nucleotide


Download         Length: 882 bp        

>NTDB_id=1158456 QEN43_RS00725 WP_036268865.1 160820..161701(-) (pilD) [Methylocaldum szegediense isolate Msz(Nor)]
TTGGATTCATTCGTTCGGTTGCTGGCCGAAAATCCGGCTTTATTCCTGACCGTCGTCGGCGTTCTCGGGCTGATGGTCGG
CAGCTTCCTGAATGTGGTCATTCACCGGTTGCCGATCATGCTGGAAAGGAATTGGCGGCGAGAGTGTCGGGCCTTTCTCG
ACCTTGAACCCGAACCGGAACCTGATCAGCCTTACAACCTGCTCGTTCCAGGGTCTCGGTGCCCTCGTTGCGATCGGCCT
ATCCGGGCGTGGGAAAACATTCCCATCGTAAGTTACCTGCTTCTCAAAAGACGATGCAAGGGGTGCCGAACCCCGATTCC
GTTTCGCTACCCGGCCATCGAGGCGCTGACCGCTTTGATCTCCATAGCCGTCGCCTGGCATTTCGGCTTCACATGGCAAA
CTGCATCCGCGCTGATCCTCAGCTGGAGTTTGATATGCTTGAGTTTCATTGATTTCGATCGCCAACTGCTTCCCGATGCG
ATTACCCTACCCGTCCTCTGGACAGGACTGTTTCTCAATGTGTTCAACATATTCACCGACAGCGCTTCGAGTATACTCGG
GGCCATCTTCGGCTACCTCAGCCTCTGGCTGGTGTATCATGTGTTCCGGCTAGCAACCGGGAAAGAAGGAATGGGATACG
GCGATTTCAAATTGCTCGCCATGCTGGGTGCCTGGTTGGGATGGAGCCTGCTACCGGTTGTAGTGCTTCTGTCCTCCCTG
GTCGGCGCCGTGATTGGGACGACGATGGTCGCGGTTTTCAGACGCGACCGCAGCATTCCCATACCGTTCGGTCCCTATTT
GGCTATCGCAGGCTGGATTGCCTTGATGTGGGGCGATGACATCAACGCCGCTTATCTCAATTTTGCGGGAATTTCCGGCT
AA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)

Type IV leader peptidase family (Peptidase_A24)

(138-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

50.903

94.539

0.481
  pilD Acinetobacter nosocomialis M2

49.129

97.952

0.481
  pilD Acinetobacter baumannii D1279779

49.129

97.952

0.481
  pilD Vibrio campbellii strain DS40M4

50.36

94.881

0.478
  pilD Neisseria gonorrhoeae MS11

50

89.42

0.447

Multiple sequence alignment