Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NB068_RS06075 Genome accession   NZ_OW969598
Coordinates   1238229..1239905 (-) Length   558 a.a.
NCBI ID   WP_250314389.1    Uniprot ID   -
Organism   Neisseria sp. Marseille-Q6792     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1233229..1244905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NB068_RS06055 pilG 1235134..1236366 (+) 1233 WP_250314385.1 type II secretion system F family protein Machinery gene
  NB068_RS06060 pilD 1236440..1237300 (+) 861 WP_250314386.1 A24 family peptidase Machinery gene
  NB068_RS06065 coaE 1237302..1237934 (+) 633 WP_250314387.1 dephospho-CoA kinase -
  NB068_RS06070 yacG 1237927..1238136 (+) 210 WP_250314388.1 DNA gyrase inhibitor YacG -
  NB068_RS06075 pilF 1238229..1239905 (-) 1677 WP_250314389.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NB068_RS06080 - 1239902..1240174 (-) 273 WP_250314390.1 hypothetical protein -
  NB068_RS06090 - 1240437..1240883 (-) 447 WP_250314391.1 DUF441 domain-containing protein -
  NB068_RS06095 - 1240888..1241862 (-) 975 WP_250314392.1 polyprenyl synthetase family protein -
  NB068_RS06100 rplU 1242086..1242394 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  NB068_RS06105 rpmA 1242419..1242691 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  NB068_RS06110 ubiM 1243030..1244214 (+) 1185 WP_250314393.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  NB068_RS10160 - 1244228..1244356 (+) 129 WP_003676605.1 hypothetical protein -
  NB068_RS06115 rpmG 1244427..1244582 (-) 156 WP_002212306.1 50S ribosomal protein L33 -
  NB068_RS06120 rpmB 1244611..1244844 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62148.32 Da        Isoelectric Point: 5.6932

>NTDB_id=1153543 NB068_RS06075 WP_250314389.1 1238229..1239905(-) (pilF) [Neisseria sp. Marseille-Q6792]
MSVGLLRTLVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSVLDLRHYPRHRVLMGVLTEEQ
MVEFRCVPIFRRDDKVFFAVSDPTQMPQIQKTVFASGISVELVIVEDDQLAGLLEWVGSRSTALLQEISAEQEEQESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPVAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLDAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNHGTEVDIMDVAYKEGMVDLRRSGLLKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1153543 NB068_RS06075 WP_250314389.1 1238229..1239905(-) (pilF) [Neisseria sp. Marseille-Q6792]
ATGAGCGTAGGTTTGCTGAGAACCTTGGTTCAAAACCAAGTGGTTACTGTCGAACAGGCCGAACACTATTATAAGGAGTC
GCAGGCAGGTAAAGAAGTGTTGCCGATGCTGTTTTCAGACGGCGTCATTTCGCCTAAATCCCTTGCGGCACTGATTGCGA
GGGTGTTCAGTTATTCGGTTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACTGAGGAACAG
ATGGTGGAGTTCCGTTGTGTGCCGATTTTCCGCCGGGACGACAAGGTATTTTTTGCGGTTTCTGACCCGACACAGATGCC
TCAGATTCAGAAGACTGTTTTTGCGTCAGGGATTTCGGTTGAGTTGGTGATTGTTGAAGACGATCAGCTGGCCGGGCTGT
TAGAGTGGGTGGGTTCGCGCTCGACTGCGTTGCTTCAGGAAATCAGTGCGGAACAGGAAGAGCAGGAAAGCCATACTCTG
TATATTGATAATGAAGAGGCGGAAGACGGTCCTGTACCGAGGTTTATCCATAAAACTTTGTCGGATGCTTTGCGCAGTGG
GGCATCTGATATTCATTTTGAGTTTTACGAACATAATGCGCGTATCCGGTTCCGTGTGGACGGGCAGCTTCGCGAGGTAG
TCCAGCCCCCGGTTGCGGTAAGGGGACAGCTTGCTTCTCGGATTAAGGTGATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCCCAGGACGGCAGGATGCAATTGACCTTCCAAAAGGGTGGTAAGCCTGTCGATTTCCGTGTCAGTACTTTGCCGAC
GCTGTTTGGTGAAAAGGTTGTGATGCGGATTTTGAATTCTGATGCGGCTTCTTTGAATATCGATCAACTTGGTTTCGAAC
CGTTTCAGAAGAAATTGCTGTTGGATGCCATCCACCGTCCTTACGGCATGGTGTTGGTAACCGGTCCGACAGGTTCGGGA
AAGACTGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGATCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCAACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAAG
AAGTGGAACGTCCGTCTGCCTCTGCTTTGAAGGAAGTCGGTTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCCGGTGTTTATGAGGTTATGCCTATCAGCGA
GGAAATGCAGCGTGTCATTATGAACCATGGTACGGAAGTGGATATTATGGATGTTGCTTATAAGGAAGGTATGGTTGATT
TGCGCCGTTCCGGTTTGTTGAAAGTTATGCAGGGTATTACTTCGTTGGAAGAGGTAACGGCAAATACTAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

95.699

100

0.957
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Acinetobacter baumannii D1279779

49.736

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.803

100

0.487
  pilB Vibrio cholerae strain A1552

46.085

100

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

45.035

100

0.455
  pilB Vibrio campbellii strain DS40M4

44.149

100

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

37.113

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.131

95.878

0.366
  pilF Thermus thermophilus HB27

36.949

97.491

0.36

Multiple sequence alignment