Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QM552_RS10360 Genome accession   NZ_OW011623
Coordinates   2268393..2270105 (-) Length   570 a.a.
NCBI ID   WP_004261084.1    Uniprot ID   -
Organism   Thauera humireducens strain Piv1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2263393..2275105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QM552_RS10340 (C4PIVTH_2151) - 2265197..2265820 (-) 624 WP_282810208.1 DUF502 domain-containing protein -
  QM552_RS10345 (C4PIVTH_2152) - 2265878..2266141 (-) 264 WP_048705998.1 FmdB family zinc ribbon protein -
  QM552_RS10350 (C4PIVTH_2153) pilD 2266288..2267139 (-) 852 WP_282810209.1 A24 family peptidase Machinery gene
  QM552_RS10355 (C4PIVTH_2154) pilC 2267143..2268372 (-) 1230 WP_038011757.1 type II secretion system F family protein Machinery gene
  QM552_RS10360 (C4PIVTH_2155) pilB 2268393..2270105 (-) 1713 WP_004261084.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QM552_RS10365 (C4PIVTH_2156) - 2270223..2271053 (-) 831 WP_282810210.1 inner membrane protein YpjD -
  QM552_RS10370 (C4PIVTH_2157) ffh 2271105..2272475 (+) 1371 WP_282810211.1 signal recognition particle protein -
  QM552_RS10375 (C4PIVTH_2158) - 2272489..2273265 (+) 777 WP_048705991.1 EI24 domain-containing protein -
  QM552_RS10380 (C4PIVTH_2159) - 2273262..2274011 (+) 750 WP_282810212.1 molybdopterin-binding protein -
  QM552_RS10385 (C4PIVTH_2160) - 2274008..2274790 (+) 783 WP_004261067.1 M48 family metallopeptidase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62002.10 Da        Isoelectric Point: 5.2332

>NTDB_id=1152203 QM552_RS10360 WP_004261084.1 2268393..2270105(-) (pilB) [Thauera humireducens strain Piv1]
MAANPQPALSGFARALIQHGRLSEADALACTAQASDGTNAFILEVAARGLMSTAAIARFAADTFGYPLLDIAAFDKSMFA
SDAVDRKLMHKHQALALAKRQNRVTLAIADPSNMRALDEIRFQTGMQLDLVIAEADKLKRVADAQNESTEQTLKELTGDE
FDMDLLKEEAPSEHKEEDTSDVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGVLREIAQPPLVLKEKIA
ARIKVISRLDISEKRVPQDGRMKLVLSKNKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGIDALGYEPDQRKALMEAIER
PYGMILVTGPTGSGKTVSLYTCLNILNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFSAALRAFLRQDPDVIMVGEI
RDLDTAEISVKAAQTGHLVLSTLHTNDAPTTLERLKNMGVAPFNIASSVILITAQRLARRLCSCKKPADIPLEALLEAGF
SPDELDGSWQPYAPGGCDRCKGSGYKGRVGIYQVMPITEEIAHIIMTGGNSMDIAAQAESEGVRDLRKSGLLKVKQGVTS
LEEVLATTNE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1152203 QM552_RS10360 WP_004261084.1 2268393..2270105(-) (pilB) [Thauera humireducens strain Piv1]
ATGGCGGCGAATCCCCAACCTGCCCTGAGCGGTTTCGCTCGGGCCCTCATCCAGCATGGACGGCTTTCCGAAGCAGACGC
ACTCGCGTGTACCGCGCAGGCCAGCGACGGCACCAATGCATTCATCCTGGAGGTCGCGGCACGCGGCCTGATGTCGACTG
CGGCGATCGCACGTTTTGCGGCCGACACTTTCGGCTACCCCCTGCTCGACATCGCCGCATTCGACAAGTCGATGTTCGCC
AGCGATGCGGTCGACCGCAAGCTGATGCACAAGCACCAGGCGCTCGCGCTCGCCAAGCGCCAGAACCGTGTCACGCTTGC
CATCGCCGATCCCTCCAACATGCGCGCGCTGGACGAGATCCGCTTCCAGACCGGCATGCAGCTCGACCTCGTGATCGCCG
AGGCCGACAAGCTCAAGCGCGTTGCCGATGCACAGAACGAGTCGACCGAGCAGACGCTGAAGGAGCTGACGGGCGACGAG
TTCGACATGGACCTGCTGAAGGAAGAGGCGCCTTCGGAGCACAAGGAAGAGGACACCAGCGACGTCGACGACGCTCCGGT
GGTCAAGTTCATCCAGAAGGTGCTGATCGACGCGATCAACGAAGGTGCGTCCGACATCCACTTCGAACCCTACGAGAAGT
ATTACCGCATCCGGGTACGGACCGACGGCGTGCTGCGTGAGATCGCGCAGCCGCCGCTGGTACTGAAGGAAAAGATCGCC
GCACGGATCAAGGTCATCTCGCGCCTCGACATTTCGGAGAAACGTGTCCCGCAAGACGGACGCATGAAGCTCGTGCTGTC
GAAGAACAAGGCGATCGACTTCCGTGTCTCGACGCTTCCGACCCTGCACGGCGAGAAGATCGTGATGCGTATTCTCGACC
CGAGTTCGGCGATGCTCGGCATCGATGCCCTGGGGTACGAGCCTGACCAGCGCAAGGCGCTGATGGAAGCGATCGAGCGC
CCCTACGGCATGATCCTGGTCACCGGCCCGACCGGCTCCGGCAAGACAGTGTCGCTCTACACCTGCCTCAATATCCTCAA
CAAGGCGGGCGTCAATATCTCGACCGCGGAAGATCCGGCCGAAATCAACCTGCCCGGCATCAACCAGGTCAACGTCAACG
AAAAGGCCGGCCTCACCTTCTCGGCGGCGCTGCGTGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTGGGTGAAATC
CGCGACCTCGACACGGCCGAGATCTCGGTCAAGGCGGCGCAGACGGGCCACCTGGTGCTGTCGACCCTGCACACCAACGA
CGCACCGACCACCCTCGAGCGCCTCAAGAACATGGGTGTCGCGCCGTTCAACATCGCGTCATCGGTGATACTGATCACGG
CCCAGCGTCTGGCGCGGCGGTTATGCTCGTGCAAGAAGCCGGCCGACATACCGCTCGAAGCCCTGCTCGAGGCGGGCTTC
AGCCCGGATGAACTCGATGGCAGCTGGCAGCCCTACGCGCCCGGCGGCTGCGACCGCTGCAAGGGCAGCGGATACAAGGG
CCGCGTGGGTATTTACCAGGTGATGCCTATTACGGAAGAGATTGCGCATATCATAATGACCGGTGGCAATTCGATGGACA
TTGCCGCCCAGGCAGAAAGCGAGGGCGTGCGCGACCTGCGCAAGTCCGGCCTGCTCAAGGTCAAACAAGGCGTGACCTCG
CTAGAGGAAGTGCTGGCGACAACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.098

100

0.565
  pilB Acinetobacter baylyi ADP1

55.477

99.298

0.551
  pilB Legionella pneumophila strain ERS1305867

52.734

99.474

0.525
  pilF Neisseria gonorrhoeae MS11

52.043

98.772

0.514
  pilB Vibrio cholerae strain A1552

53.057

88.947

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

49.522

91.754

0.454
  pilB Vibrio campbellii strain DS40M4

48.952

92.105

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

40.691

91.404

0.372
  pilF Thermus thermophilus HB27

39.962

92.632

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

92.105

0.368

Multiple sequence alignment