Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F542_RS06250 Genome accession   NZ_CP006954
Coordinates   1373357..1374754 (+) Length   465 a.a.
NCBI ID   WP_015432350.1    Uniprot ID   A0A179D0F8
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-188     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1368357..1379754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F542_RS06230 (F542_12640) - 1368758..1369159 (-) 402 WP_015432355.1 H-NS family nucleoid-associated regulatory protein -
  F542_RS06235 (F542_12660) - 1369664..1371208 (+) 1545 WP_015432353.1 Na+/H+ antiporter NhaC family protein -
  F542_RS06240 (F542_12670) radA 1371299..1372678 (-) 1380 WP_015432352.1 DNA repair protein RadA Machinery gene
  F542_RS06245 (F542_12680) pilA 1372853..1373299 (+) 447 WP_025267127.1 prepilin peptidase-dependent pilin Machinery gene
  F542_RS06250 (F542_12690) pilB 1373357..1374754 (+) 1398 WP_015432350.1 GspE/PulE family protein Machinery gene
  F542_RS06255 (F542_12700) pilC 1374747..1375943 (+) 1197 WP_025267128.1 type II secretion system F family protein Machinery gene
  F542_RS06260 (F542_12710) - 1375940..1376605 (+) 666 WP_025267129.1 A24 family peptidase -
  F542_RS06265 (F542_12720) coaE 1376635..1377252 (+) 618 WP_042365646.1 dephospho-CoA kinase -
  F542_RS06270 (F542_12730) yacG 1377255..1377461 (+) 207 WP_015432346.1 DNA gyrase inhibitor YacG -
  F542_RS06275 (F542_12740) - 1377521..1378075 (-) 555 WP_025267130.1 hypothetical protein -
  F542_RS06280 (F542_12750) - 1378210..1379394 (-) 1185 WP_025267131.1 FAD-dependent monooxygenase -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53408.14 Da        Isoelectric Point: 5.3553

>NTDB_id=115201 F542_RS06250 WP_015432350.1 1373357..1374754(+) (pilB) [Bibersteinia trehalosi USDA-ARS-USMARC-188]
MSKYTICALNSSTQFEISERQWQKNCDEKQLLRRYLAMPLQENEQKIWLALDDENNLNACEIFTFLTHKQVEPVLVESEE
LKFLLNQLSPENEVAIDFDEFQTLHIDEQTFSDGLDFNDPIIRTLDNLFKFCLQKNASDIHIEPHKQHLMIRLRIDGVLQ
LYKTLPTHFSSRLLSRLKLLAKLDISENRLPQDGQFSFTTELNEILDFRVSTLPVQYGEKMVLRLQKNKPTHLDFLSLGF
TQAQQEKLLNALKQPQGMILVTGPTGSGKSITLYSALNYLNQSDKHILTAEDPIEIEIEGLIQTQVNHAIQLDFSRLLRT
FLRQDPDIIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAIERLNQLGIKDYEIKNSLLLVIAQRLVRLICPKCK
GEGCQECYQGYKGRIGVFQLFSRVAEQFEKSTACLDYPTLQESAKHKLAENLTDLHEIERVFGHV

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=115201 F542_RS06250 WP_015432350.1 1373357..1374754(+) (pilB) [Bibersteinia trehalosi USDA-ARS-USMARC-188]
ATGTCTAAATACACCATCTGCGCACTCAACTCATCAACCCAGTTTGAAATCAGTGAACGACAATGGCAAAAAAACTGCGA
TGAAAAGCAACTACTACGCCGTTATCTTGCTATGCCTCTGCAGGAAAATGAGCAAAAAATCTGGCTGGCGCTTGATGATG
AAAATAATCTAAATGCTTGTGAAATTTTTACTTTTCTCACGCATAAACAGGTTGAACCTGTATTAGTTGAAAGCGAGGAA
TTGAAATTTTTACTTAATCAGTTATCCCCTGAAAATGAGGTAGCAATAGACTTTGATGAATTTCAAACTCTACATATCGA
TGAGCAAACATTCTCTGACGGACTTGATTTTAATGATCCGATTATTCGCACTCTAGATAATCTATTTAAATTCTGTTTAC
AGAAAAATGCTTCTGATATTCACATTGAGCCCCATAAACAGCATTTAATGATTCGTCTGCGTATTGATGGTGTACTCCAA
CTTTATAAAACCTTACCCACCCATTTTTCAAGTCGTTTACTTTCTCGTCTGAAACTATTAGCCAAACTAGATATCAGTGA
AAATCGTTTACCACAAGATGGACAGTTTAGCTTTACTACCGAACTGAATGAAATCTTAGATTTTCGTGTTTCTACCCTAC
CTGTGCAATATGGTGAAAAAATGGTTCTGCGCTTACAAAAAAATAAACCTACTCACTTGGATTTTCTCTCGCTCGGTTTT
ACCCAAGCGCAACAAGAGAAACTGCTCAATGCTTTAAAACAACCTCAAGGAATGATTCTAGTAACAGGTCCAACTGGGAG
TGGAAAAAGTATTACGCTCTATAGTGCATTGAATTATTTAAACCAAAGCGATAAGCATATTCTTACTGCTGAAGATCCTA
TTGAAATTGAGATTGAAGGCTTAATTCAAACGCAAGTAAACCACGCTATTCAACTTGATTTTAGCCGGCTATTACGTACA
TTTCTCCGCCAAGATCCAGATATTATTATGCTGGGAGAAATTCGAGATGAAGAGAGCGCCGAAATTGCATTACGAGCAGC
ACAAACAGGCCATCTAGTGCTTTCTACTTTACATACAAATGATGCACCCTCAGCGATTGAACGCCTAAATCAACTCGGTA
TTAAAGACTATGAAATAAAAAATTCACTGTTATTAGTGATTGCTCAACGTTTGGTTCGCCTTATTTGCCCTAAATGCAAG
GGCGAAGGTTGCCAAGAATGTTATCAAGGCTACAAAGGACGAATAGGCGTATTTCAACTTTTCTCTCGTGTTGCAGAACA
GTTTGAAAAATCAACCGCTTGCTTAGACTATCCCACATTACAGGAAAGCGCAAAGCACAAATTAGCTGAAAATCTAACCG
ACTTGCATGAAATTGAACGGGTATTTGGCCATGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(133-393)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-91)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A179D0F8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

70.69

99.785

0.705
  pilB Haemophilus influenzae 86-028NP

57.363

97.849

0.561
  pilB Haemophilus influenzae Rd KW20

57.143

97.849

0.559
  pilB Vibrio cholerae strain A1552

40.871

100

0.424
  pilB Vibrio parahaemolyticus RIMD 2210633

39.2

100

0.422
  pilB Vibrio campbellii strain DS40M4

39.709

100

0.411
  pilB Legionella pneumophila strain ERS1305867

37.602

100

0.398
  pilB Acinetobacter baumannii D1279779

37.398

100

0.396
  pilB Acinetobacter baylyi ADP1

37.5

100

0.394
  pilF Thermus thermophilus HB27

35.443

100

0.361

Multiple sequence alignment