Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LQ983_RS07660 Genome accession   NZ_OV100759
Coordinates   1586918..1588327 (-) Length   469 a.a.
NCBI ID   WP_230622239.1    Uniprot ID   -
Organism   Aggregatibacter sp. Marseille-P9115     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1581918..1593327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ983_RS07630 - 1582111..1583487 (-) 1377 WP_230622233.1 sodium:alanine symporter family protein -
  LQ983_RS07635 - 1583866..1584138 (-) 273 WP_230622234.1 GNAT family N-acetyltransferase -
  LQ983_RS07640 yacG 1584135..1584350 (-) 216 WP_230622235.1 DNA gyrase inhibitor YacG -
  LQ983_RS07645 coaE 1584340..1584963 (-) 624 WP_230622236.1 dephospho-CoA kinase -
  LQ983_RS07650 - 1585013..1585705 (-) 693 WP_230622237.1 A24 family peptidase -
  LQ983_RS07655 pilC 1585702..1586925 (-) 1224 WP_230622238.1 type II secretion system F family protein Machinery gene
  LQ983_RS07660 pilB 1586918..1588327 (-) 1410 WP_230622239.1 GspE/PulE family protein Machinery gene
  LQ983_RS07665 pilA 1588356..1588808 (-) 453 WP_230622240.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LQ983_RS07670 ampD 1588935..1589489 (+) 555 WP_230622241.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LQ983_RS07675 - 1590077..1590622 (+) 546 WP_230622242.1 carboxymuconolactone decarboxylase family protein -
  LQ983_RS07680 - 1590786..1591580 (+) 795 WP_006719133.1 formate/nitrite transporter family protein -
  LQ983_RS07685 nanQ 1591677..1592147 (-) 471 WP_230622243.1 N-acetylneuraminate anomerase -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 53316.68 Da        Isoelectric Point: 6.1163

>NTDB_id=1151827 LQ983_RS07660 WP_230622239.1 1586918..1588327(-) (pilB) [Aggregatibacter sp. Marseille-P9115]
MQHPMERHYSVVNQQGEEFCITPELWLRNQQQQTALLRYLALPLKEDAQTLWLGLDSLTNLAACETFSFLSGKHIEPVLL
PSHLLKSALQDLTKSEKVEENQPLFYSTELQEQPDTQQIDEPIIQQLNQIFENATQKKASDIHLEPQAEGLQVRFRIDGV
LQVQEQISRSLANRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLATQFGEKAVLRLQQNRPVQLEFSEL
GMTESQQQLFRHALSQPQGLVLVTGPTGSGKSISLYTALQYLNTPEKHIMTAEDPIEIQLPGIIQTQVNNPIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAPSAISRLQQLGIQRHEIDSSLLLVIAQRLVRKRCQK
CGGNPNHFCDCHQGYKGRVGVYQFLQPQLHHPQGYEMDFDDLRQSALEKIRNGTTDLVEIQRVLGQSHD

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=1151827 LQ983_RS07660 WP_230622239.1 1586918..1588327(-) (pilB) [Aggregatibacter sp. Marseille-P9115]
ATGCAACATCCGATGGAACGGCATTATTCGGTCGTCAATCAACAGGGCGAAGAATTTTGTATTACGCCTGAGCTTTGGCT
TAGAAATCAGCAACAACAAACCGCCCTCTTACGTTATTTGGCGTTACCGTTAAAAGAAGACGCCCAAACTCTGTGGCTAG
GATTGGATTCATTAACCAATCTTGCCGCTTGTGAAACCTTTTCCTTTCTCAGTGGAAAGCACATTGAGCCTGTGTTATTG
CCTAGCCATCTACTCAAAAGCGCACTACAGGATTTAACAAAATCTGAAAAAGTCGAGGAAAATCAACCGCTCTTTTATTC
AACTGAACTACAAGAACAGCCTGATACCCAGCAAATTGATGAGCCGATCATTCAACAACTCAATCAGATTTTTGAAAACG
CTACACAGAAAAAAGCATCCGATATTCATTTAGAACCGCAAGCCGAGGGATTACAAGTGCGTTTTCGTATTGATGGCGTA
TTGCAAGTACAAGAACAAATCTCGCGTTCTTTGGCAAACCGCTTAATTTCCCGTCTGAAATTATTGGCGAAATTGGATAT
TAGCGAGACACGTTTACCGCAAGATGGCCGCTTTCAGTTTAAAACCACCTTTTCCGATATTTTAGATTTTCGTCTCTCAA
CCCTTGCGACCCAATTTGGCGAAAAAGCCGTGTTGCGTTTACAGCAAAATCGGCCCGTTCAGTTGGAATTCAGTGAATTG
GGCATGACTGAATCACAGCAACAACTTTTCCGTCATGCATTAAGCCAACCCCAAGGGTTGGTTTTAGTAACCGGCCCGAC
CGGTAGCGGTAAAAGTATTTCTTTGTATACAGCATTACAGTATTTAAATACACCGGAAAAACACATTATGACGGCAGAGG
ATCCCATTGAAATTCAGTTGCCAGGTATTATTCAAACTCAAGTAAATAATCCTATTGGCTTGGATTTCAGCCGTCTCCTT
CGTACCTTCTTACGCCAAGATCCCGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCCGCCATGGCACTACG
CGCCGCACAAACCGGACACTTAGTCCTTTCTACCTTACACACCAATGATGCTCCTTCCGCCATTTCCCGCTTGCAACAGT
TGGGTATTCAGCGCCATGAAATCGACAGCAGCTTGTTACTTGTCATCGCGCAACGATTGGTGCGTAAACGTTGCCAAAAG
TGCGGTGGAAATCCCAACCATTTTTGCGACTGTCATCAAGGTTACAAAGGGCGTGTCGGGGTATATCAATTCCTACAACC
TCAACTACACCATCCGCAAGGCTATGAAATGGATTTCGATGATTTACGCCAAAGCGCCTTAGAAAAAATCCGAAACGGTA
CAACAGATTTAGTCGAAATTCAGCGCGTATTAGGGCAGTCCCATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(33-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

71.772

97.441

0.699
  pilB Haemophilus influenzae Rd KW20

71.335

97.441

0.695
  pilB Glaesserella parasuis strain SC1401

56.129

99.147

0.557
  pilB Vibrio campbellii strain DS40M4

41.632

100

0.424
  pilB Vibrio parahaemolyticus RIMD 2210633

41.632

100

0.424
  pilB Vibrio cholerae strain A1552

41.3

100

0.42
  pilB Acinetobacter baylyi ADP1

38.755

100

0.412
  pilB Acinetobacter baumannii D1279779

39.669

100

0.409
  pilB Legionella pneumophila strain ERS1305867

38.076

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.764

100

0.382

Multiple sequence alignment