Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N900_RS01375 Genome accession   NZ_CP006947
Coordinates   281031..282746 (+) Length   571 a.a.
NCBI ID   WP_000788426.1    Uniprot ID   A0A0K9UP29
Organism   Vibrio cholerae O1 str. KW3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 276031..287746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N900_RS01355 (N900_01335) pilM 278324..279340 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  N900_RS01360 (N900_01340) pilN 279330..279914 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  N900_RS01365 (N900_01345) pilO 279907..280497 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  N900_RS01370 (N900_01350) pilP 280487..281005 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  N900_RS01375 (N900_01355) pilQ 281031..282746 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  N900_RS01380 (N900_01360) aroK 282933..283457 (+) 525 WP_000818616.1 shikimate kinase AroK -
  N900_RS01385 (N900_01365) aroB 283485..284570 (+) 1086 WP_000439805.1 3-dehydroquinate synthase -
  N900_RS01390 (N900_01370) - 284625..286100 (+) 1476 WP_000050195.1 ExeA family protein -
  N900_RS01395 (N900_01375) - 286170..287003 (+) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62340.49 Da        Isoelectric Point: 4.6119

>NTDB_id=115091 N900_RS01375 WP_000788426.1 281031..282746(+) (pilQ) [Vibrio cholerae O1 str. KW3]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=115091 N900_RS01375 WP_000788426.1 281031..282746(+) (pilQ) [Vibrio cholerae O1 str. KW3]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTAAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCCGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTGATTTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAACGGGTCGATGGTAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTTAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAATATTGCTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTGATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTAAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTCGACTTG
AGTGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATCGATCGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGGGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K9UP29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

100

100

1
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilQ Vibrio campbellii strain DS40M4

78.545

96.322

0.757
  pilQ Legionella pneumophila strain ERS1305867

42.778

94.571

0.405
  pilQ Legionella pneumophila strain Lp02

42.778

94.571

0.405
  comQ Acinetobacter baylyi ADP1

39.336

100

0.394
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Pseudomonas aeruginosa PAK

39.344

96.147

0.378

Multiple sequence alignment