Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HUW69_RS02450 Genome accession   NZ_LT992490
Coordinates   523607..524833 (-) Length   408 a.a.
NCBI ID   WP_084988583.1    Uniprot ID   -
Organism   Vibrio cholerae strain 4295STDY6534200     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 518607..529833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW69_RS02425 (SAMEA4374365_00496) parC 518608..520893 (+) 2286 WP_000102504.1 DNA topoisomerase IV subunit A -
  HUW69_RS02430 (SAMEA4374365_00497) yacG 520942..521139 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HUW69_RS02435 (SAMEA4374365_00498) zapD 521297..522037 (-) 741 WP_000207194.1 cell division protein ZapD -
  HUW69_RS02440 (SAMEA4374365_00499) coaE 522069..522677 (-) 609 WP_000011549.1 dephospho-CoA kinase -
  HUW69_RS02445 (SAMEA4374365_00500) pilD 522674..523549 (-) 876 WP_000418750.1 prepilin peptidase Machinery gene
  HUW69_RS02450 (SAMEA4374365_00501) pilC 523607..524833 (-) 1227 WP_084988583.1 type II secretion system F family protein Machinery gene
  HUW69_RS02455 (SAMEA4374365_00502) pilB 524876..526564 (-) 1689 WP_032481238.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUW69_RS02460 (SAMEA4374365_00503) - 526564..527049 (-) 486 WP_000649332.1 pilin -
  HUW69_RS02465 (SAMEA4374365_00504) nadC 527294..528184 (-) 891 WP_084988582.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUW69_RS02470 (SAMEA4374365_00505) ampD 528482..529027 (+) 546 WP_084988581.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUW69_RS02475 (SAMEA4374365_00506) fldB 529305..529823 (-) 519 WP_084988580.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45108.40 Da        Isoelectric Point: 10.3744

>NTDB_id=1150264 HUW69_RS02450 WP_084988583.1 523607..524833(-) (pilC) [Vibrio cholerae strain 4295STDY6534200]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVHRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1150264 HUW69_RS02450 WP_084988583.1 523607..524833(-) (pilC) [Vibrio cholerae strain 4295STDY6534200]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTCTCTTTATTGGCGCGCCTAACCCATCGCGTGAAAAGCAAAGACATCACGATTTTGACTCGGCAGTTGGCAACCATG
CTCACCACGGGCGTACCCATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAAGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTGGTGTTGGTCGCGCTCGGAGTTTC
CTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACCC
AGCAAGTACTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCTTTCCAGATCCGTTTAAAAACCAGCCGCTTGGGGCTGAAATTTCCGATTATTGGCAA
TGTACTCGCCAAAGCGTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCAATTCTCGCCA
GTTTAAAAACCACCGCCAAAACCTCCGGCAATGTGCACTTTGAAACTGCGATTAATGAGGTACATCGTGATACTGCCGCC
GGTATGCCAATGTACATTGCCATGCGCAATACCGAAGCCTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGTCAATTAGATGACATGCTCAACAAGGTCGCAACCATCTATGAATTTGAAGTCGATAACACAGTCGATAACTTAG
GCAAAATTCTTGAGCCGCTGATCATCGTCTTTCTTGGGACAGTCGTGGGCGGTTTGGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.755

100

0.998
  pilC Vibrio campbellii strain DS40M4

73.5

98.039

0.721
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402

Multiple sequence alignment