Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HUW69_RS02445 Genome accession   NZ_LT992490
Coordinates   522674..523549 (-) Length   291 a.a.
NCBI ID   WP_000418750.1    Uniprot ID   -
Organism   Vibrio cholerae strain 4295STDY6534200     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 517674..528549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW69_RS02425 (SAMEA4374365_00496) parC 518608..520893 (+) 2286 WP_000102504.1 DNA topoisomerase IV subunit A -
  HUW69_RS02430 (SAMEA4374365_00497) yacG 520942..521139 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HUW69_RS02435 (SAMEA4374365_00498) zapD 521297..522037 (-) 741 WP_000207194.1 cell division protein ZapD -
  HUW69_RS02440 (SAMEA4374365_00499) coaE 522069..522677 (-) 609 WP_000011549.1 dephospho-CoA kinase -
  HUW69_RS02445 (SAMEA4374365_00500) pilD 522674..523549 (-) 876 WP_000418750.1 prepilin peptidase Machinery gene
  HUW69_RS02450 (SAMEA4374365_00501) pilC 523607..524833 (-) 1227 WP_084988583.1 type II secretion system F family protein Machinery gene
  HUW69_RS02455 (SAMEA4374365_00502) pilB 524876..526564 (-) 1689 WP_032481238.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUW69_RS02460 (SAMEA4374365_00503) - 526564..527049 (-) 486 WP_000649332.1 pilin -
  HUW69_RS02465 (SAMEA4374365_00504) nadC 527294..528184 (-) 891 WP_084988582.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32461.75 Da        Isoelectric Point: 7.1618

>NTDB_id=1150263 HUW69_RS02445 WP_000418750.1 522674..523549(-) (pilD) [Vibrio cholerae strain 4295STDY6534200]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMVGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=1150263 HUW69_RS02445 WP_000418750.1 522674..523549(-) (pilD) [Vibrio cholerae strain 4295STDY6534200]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCCGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTAGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGTTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCTACTCACTTTCCGTTCGGGGTGTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTGCGGCAACCTTCATCGACTTCGATACCCTGTTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCGGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGT
GGGTTATCTCTCGCTGTGGTCAATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCTCTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(135-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

99.656

100

0.997
  pilD Vibrio campbellii strain DS40M4

75

98.969

0.742
  pilD Acinetobacter nosocomialis M2

53.676

93.471

0.502
  pilD Acinetobacter baumannii D1279779

53.309

93.471

0.498
  pilD Neisseria gonorrhoeae MS11

44.444

98.969

0.44

Multiple sequence alignment