Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LSAJ54_RS06775 Genome accession   NZ_LT960790
Coordinates   1344081..1344971 (-) Length   296 a.a.
NCBI ID   WP_099947399.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain J54     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1339081..1349971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSAJ54_RS06740 (LSAJ54_1366) - 1340147..1341157 (-) 1011 WP_025016388.1 class I SAM-dependent methyltransferase -
  LSAJ54_RS06745 (LSAJ54_1367) - 1341281..1341619 (-) 339 WP_056947123.1 hypothetical protein -
  LSAJ54_RS06750 (LSAJ54_1368) comGF 1341582..1342094 (-) 513 WP_035145013.1 competence type IV pilus minor pilin ComGF Machinery gene
  LSAJ54_RS06755 (LSAJ54_1369) comGE 1342069..1342374 (-) 306 WP_011374997.1 hypothetical protein Machinery gene
  LSAJ54_RS06760 (LSAJ54_1370) comGD 1342361..1342813 (-) 453 WP_035145016.1 competence type IV pilus minor pilin ComGD Machinery gene
  LSAJ54_RS06765 (LSAJ54_1371) comGC 1342785..1343084 (-) 300 WP_011374999.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LSAJ54_RS06770 (LSAJ54_1372) comGB 1343081..1344088 (-) 1008 WP_056947133.1 type II secretion system F family protein Machinery gene
  LSAJ54_RS06775 (LSAJ54_1373) comGA 1344081..1344971 (-) 891 WP_099947399.1 competence type IV pilus ATPase ComGA Machinery gene
  LSAJ54_RS06780 (LSAJ54_1374) - 1345087..1345818 (-) 732 WP_076632059.1 YebC/PmpR family DNA-binding transcriptional regulator -
  LSAJ54_RS06785 (LSAJ54_1375) - 1345909..1346409 (-) 501 WP_076632058.1 VanZ family protein -
  LSAJ54_RS06790 (LSAJ54_1376) - 1346506..1346880 (+) 375 WP_099947400.1 hypothetical protein -
  LSAJ54_RS06800 (LSAJ54_1377) - 1347067..1348467 (-) 1401 WP_099947401.1 hypothetical protein -
  LSAJ54_RS06805 (LSAJ54_1378) - 1348467..1349537 (-) 1071 WP_157771032.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32922.77 Da        Isoelectric Point: 6.4932

>NTDB_id=1148516 LSAJ54_RS06775 WP_099947399.1 1344081..1344971(-) (comGA) [Latilactobacillus sakei strain J54]
MSTEEKLTTLLKHCAKQRVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSTQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGPDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=1148516 LSAJ54_RS06775 WP_099947399.1 1344081..1344971(-) (comGA) [Latilactobacillus sakei strain J54]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAACAACGTGTTCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGATCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGTAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTTAATGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAAATTCGCGATTTAG
AGACAGCTCAATATGCAGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCAGAGCTGCAATTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTCCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

98.986

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.642

89.527

0.382
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

40.876

92.568

0.378
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae R6

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae D39

42.264

89.527

0.378
  comGA Lactococcus lactis subsp. cremoris KW2

37.458

100

0.378
  comYA Streptococcus gordonii str. Challis substr. CH1

40.59

91.554

0.372

Multiple sequence alignment