Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CKW06_RS05450 Genome accession   NZ_LT906480
Coordinates   1143431..1144525 (+) Length   364 a.a.
NCBI ID   WP_043033789.1    Uniprot ID   A0A0X3QW38
Organism   Stenotrophomonas maltophilia strain NCTC10257     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1138431..1149525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW06_RS05425 (SAMEA4076705_01102) - 1138887..1139489 (-) 603 WP_024956083.1 short chain dehydrogenase -
  CKW06_RS05430 (SAMEA4076705_01103) - 1139588..1140463 (+) 876 WP_024956082.1 LysR family transcriptional regulator -
  CKW06_RS05435 (SAMEA4076705_01104) proC 1140640..1141461 (-) 822 WP_024956081.1 pyrroline-5-carboxylate reductase -
  CKW06_RS05440 (SAMEA4076705_01105) - 1141480..1142157 (-) 678 WP_024956080.1 YggS family pyridoxal phosphate-dependent enzyme -
  CKW06_RS05445 (SAMEA4076705_01106) pilT 1142245..1143282 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  CKW06_RS05450 (SAMEA4076705_01107) pilU 1143431..1144525 (+) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CKW06_RS05455 (SAMEA4076705_01108) - 1144733..1145716 (+) 984 WP_024956079.1 aldo/keto reductase -
  CKW06_RS05460 (SAMEA4076705_01109) - 1145764..1146663 (-) 900 WP_024956078.1 LysR family transcriptional regulator -
  CKW06_RS05465 (SAMEA4076705_01110) - 1146757..1146966 (+) 210 WP_005412490.1 DUF4287 domain-containing protein -
  CKW06_RS05470 (SAMEA4076705_01111) - 1147077..1147784 (+) 708 WP_024956077.1 YitT family protein -
  CKW06_RS05475 (SAMEA4076705_01112) - 1147868..1148413 (-) 546 WP_024956076.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40569.61 Da        Isoelectric Point: 6.7680

>NTDB_id=1148071 CKW06_RS05450 WP_043033789.1 1143431..1144525(+) (pilU) [Stenotrophomonas maltophilia strain NCTC10257]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=1148071 CKW06_RS05450 WP_043033789.1 1143431..1144525(+) (pilU) [Stenotrophomonas maltophilia strain NCTC10257]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAAT
TCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGA
TGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCACGAA
GGCTGCATCATCACCCAGCGTGAGGTCGGCATCGATACCGACAGCTGGGAAGCCGCGTTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATCGGTGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAGACCGGTCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTC
GCGAAAGGTCGCCATGGAGATCCTGCTCGGCACGCCGCTGGTTCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAG
ATCAGCTACGAGGACGCGCTGCGTTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCACGCACGCTGTCACAGGGCCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X3QW38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363

Multiple sequence alignment