Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CKW04_RS08575 Genome accession   NZ_LT906442
Coordinates   1845640..1846860 (+) Length   406 a.a.
NCBI ID   WP_058481708.1    Uniprot ID   A0A0W1A238
Organism   Legionella waltersii strain NCTC13017     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1840640..1851860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW04_RS08550 (SAMEA4504053_01750) - 1840735..1841868 (+) 1134 WP_058481713.1 HlyD family type I secretion periplasmic adaptor subunit -
  CKW04_RS08555 (SAMEA4504053_01751) - 1841871..1842440 (-) 570 WP_058481712.1 hypoxanthine-guanine phosphoribosyltransferase -
  CKW04_RS08560 (SAMEA4504053_01752) - 1842454..1842780 (-) 327 WP_058481711.1 DUF1820 family protein -
  CKW04_RS08565 (SAMEA4504053_01753) - 1842940..1843683 (-) 744 WP_058481710.1 methyltransferase domain-containing protein -
  CKW04_RS08570 (SAMEA4504053_01754) pilB 1843913..1845634 (+) 1722 WP_058481709.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKW04_RS08575 (SAMEA4504053_01755) pilC 1845640..1846860 (+) 1221 WP_058481708.1 type II secretion system F family protein Machinery gene
  CKW04_RS08580 (SAMEA4504053_01756) - 1846876..1847748 (+) 873 WP_058481707.1 A24 family peptidase -
  CKW04_RS08585 (SAMEA4504053_01757) - 1847711..1848313 (-) 603 WP_058481706.1 hypothetical protein -
  CKW04_RS08590 (SAMEA4504053_01758) - 1848329..1848775 (-) 447 WP_058481705.1 hypothetical protein -
  CKW04_RS08595 (SAMEA4504053_01759) - 1848912..1849727 (+) 816 WP_058481704.1 pyruvate, water dikinase regulatory protein -
  CKW04_RS08600 (SAMEA4504053_01760) - 1849942..1850388 (+) 447 WP_058481703.1 hypothetical protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44571.52 Da        Isoelectric Point: 9.3708

>NTDB_id=1147238 CKW04_RS08575 WP_058481708.1 1845640..1846860(+) (pilC) [Legionella waltersii strain NCTC13017]
MEKIKNPLLTFHYHGTNKSGQTMEGDIQAKSMAIAKADLRKQGIVATKIAKKRKPLIDRKNKKITQADITVFSRQLATMI
ESGIPLVQAFDIVAKGQSNKRLKDLIETIKQDIETGLTLAESFIKHPQYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
IETIKKKIKKALTYPIAVLVVALIVTTGLLIYVVPQFESLFKGFGADLPAMTKAVISMSEFMQSYWYFIFGALGGAVYAF
IYAKNHSLDFAQNIDRAMLKFPVVGVILEKASIARFARTLSITFAAGLPLVEALKSVSGATGNIIYAKATDKIRDEVSTG
QQMCVAMENTHLFPNMVIQMVAIGEESGALEKMLGKVADFYEEEVDNAVDSLSSLMEPIIMSILGILVGGLVVAMYLPIF
KLGSAV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1147238 CKW04_RS08575 WP_058481708.1 1845640..1846860(+) (pilC) [Legionella waltersii strain NCTC13017]
ATGGAAAAGATTAAAAATCCACTTCTTACCTTTCATTATCATGGTACGAATAAATCTGGCCAGACTATGGAGGGAGACAT
TCAAGCTAAAAGTATGGCTATTGCAAAAGCTGATCTACGTAAACAAGGAATTGTTGCAACTAAAATCGCTAAAAAGAGAA
AGCCCCTCATTGATAGAAAAAATAAAAAGATTACACAAGCAGATATCACCGTTTTTAGCCGTCAATTAGCAACGATGATT
GAATCAGGAATTCCACTGGTCCAAGCATTTGATATCGTTGCCAAAGGTCAATCGAATAAACGTTTAAAAGACCTAATTGA
AACAATTAAGCAAGACATCGAGACTGGTTTAACCTTGGCTGAATCTTTTATTAAGCACCCTCAATATTTTAATGAATTAT
TTTGTAATTTAGTGGATGCTGGTGAAAAGTCAGGTTCTTTAGATATTATGTTGGATAAGGTAGCAACCTATAAAGAAAAA
ATTGAAACCATTAAGAAAAAAATTAAAAAAGCGCTCACTTACCCAATTGCAGTATTGGTGGTAGCACTTATAGTGACAAC
TGGACTGTTAATCTATGTAGTCCCTCAATTTGAGTCCTTATTTAAAGGTTTTGGTGCTGACTTACCTGCTATGACAAAGG
CCGTAATTTCTATGTCTGAATTCATGCAATCCTATTGGTATTTCATCTTTGGGGCACTTGGTGGTGCAGTATATGCGTTT
ATCTATGCCAAAAATCATTCTTTGGACTTTGCACAGAACATAGATCGCGCCATGCTTAAATTCCCGGTTGTAGGGGTGAT
TTTAGAAAAGGCATCCATTGCGCGTTTTGCTAGAACATTGTCAATTACGTTTGCAGCTGGACTACCATTAGTAGAAGCCT
TGAAGTCAGTTTCTGGCGCAACGGGTAATATCATATATGCAAAAGCAACGGATAAAATAAGAGATGAAGTATCAACCGGT
CAACAAATGTGTGTAGCTATGGAAAACACACATCTATTCCCCAACATGGTTATACAAATGGTAGCTATAGGTGAAGAGTC
TGGTGCATTGGAGAAGATGTTAGGAAAGGTAGCCGATTTCTATGAAGAAGAAGTTGATAATGCCGTCGATTCACTAAGCA
GTTTAATGGAGCCAATAATTATGTCAATATTAGGCATATTGGTTGGTGGTTTAGTTGTAGCTATGTATCTACCTATTTTC
AAATTGGGATCAGCAGTCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W1A238

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

88.916

100

0.889
  pilC Pseudomonas stutzeri DSM 10701

54.839

99.261

0.544
  pilC Acinetobacter baumannii D1279779

54.271

98.03

0.532
  pilC Acinetobacter baylyi ADP1

53.904

97.783

0.527
  pilG Neisseria meningitidis 44/76-A

46.096

97.783

0.451
  pilG Neisseria gonorrhoeae MS11

45.844

97.783

0.448
  pilC Vibrio cholerae strain A1552

41

98.522

0.404
  pilC Vibrio campbellii strain DS40M4

38.424

100

0.384

Multiple sequence alignment