Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CKW04_RS08570 Genome accession   NZ_LT906442
Coordinates   1843913..1845634 (+) Length   573 a.a.
NCBI ID   WP_058481709.1    Uniprot ID   A0A0W1A169
Organism   Legionella waltersii strain NCTC13017     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1838913..1850634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW04_RS08550 (SAMEA4504053_01750) - 1840735..1841868 (+) 1134 WP_058481713.1 HlyD family type I secretion periplasmic adaptor subunit -
  CKW04_RS08555 (SAMEA4504053_01751) - 1841871..1842440 (-) 570 WP_058481712.1 hypoxanthine-guanine phosphoribosyltransferase -
  CKW04_RS08560 (SAMEA4504053_01752) - 1842454..1842780 (-) 327 WP_058481711.1 DUF1820 family protein -
  CKW04_RS08565 (SAMEA4504053_01753) - 1842940..1843683 (-) 744 WP_058481710.1 methyltransferase domain-containing protein -
  CKW04_RS08570 (SAMEA4504053_01754) pilB 1843913..1845634 (+) 1722 WP_058481709.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKW04_RS08575 (SAMEA4504053_01755) pilC 1845640..1846860 (+) 1221 WP_058481708.1 type II secretion system F family protein Machinery gene
  CKW04_RS08580 (SAMEA4504053_01756) - 1846876..1847748 (+) 873 WP_058481707.1 A24 family peptidase -
  CKW04_RS08585 (SAMEA4504053_01757) - 1847711..1848313 (-) 603 WP_058481706.1 hypothetical protein -
  CKW04_RS08590 (SAMEA4504053_01758) - 1848329..1848775 (-) 447 WP_058481705.1 hypothetical protein -
  CKW04_RS08595 (SAMEA4504053_01759) - 1848912..1849727 (+) 816 WP_058481704.1 pyruvate, water dikinase regulatory protein -
  CKW04_RS08600 (SAMEA4504053_01760) - 1849942..1850388 (+) 447 WP_058481703.1 hypothetical protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63060.58 Da        Isoelectric Point: 5.1517

>NTDB_id=1147237 CKW04_RS08570 WP_058481709.1 1843913..1845634(+) (pilB) [Legionella waltersii strain NCTC13017]
MALATDEQRLQGIGQLLVLEKLLEKSKAIELYKAAASEKISLIQYIVKNRILTAEQIALTAAQNFGVPMLDLLCIDLDNL
PAKLVNEKLIKRHSMVPLFSRGNNLYLATDDPSKQASLKEIQFHTGLNTNAIVVETDKLSALIDKLLTAKESEGLSEYVE
DAGDIDGLEISTDEEDQDSDVSSVTDDAPIVKFINKILLDAIKQGTSDIHFEPYEKEYRIRYRQDGILHEVAAPPVNLSA
RITARIKVMSNLDISERRVPQDGGFKMKISKNRAIDFRVSTCPTYTGEKVVMRILDPSTAKLGVDALGFAQYQKEAFIRA
IEKPQGMILVTGPTGSGKTVTLYTALNMLNTTEVNISTAEDPVEIKVPGINQVNINTKAGLTFSGALRSFLRQDPDIIMV
GEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARRLCEQCKVLRDDVSDKGL
LELGFKEEDLVGLKLYKAVGCNQCTNGFRGRVGLYEVLTMNKDLGQMIMNGANSLEILKFAQSQGMHTIFESGLEKVKEG
VTTMEEVNRVTVD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1147237 CKW04_RS08570 WP_058481709.1 1843913..1845634(+) (pilB) [Legionella waltersii strain NCTC13017]
ATGGCACTTGCTACTGACGAACAACGACTACAAGGCATTGGGCAACTTCTTGTGCTTGAAAAGCTTTTAGAGAAATCTAA
AGCTATAGAGCTCTATAAAGCCGCTGCTTCTGAAAAAATCTCTCTTATACAATACATAGTAAAAAATCGCATATTAACGG
CTGAGCAAATTGCACTTACTGCAGCTCAAAATTTTGGTGTACCCATGCTCGATCTATTATGTATTGATTTAGATAATTTA
CCAGCAAAATTAGTCAATGAGAAACTGATAAAGCGGCACTCGATGGTTCCATTATTTAGTCGAGGAAATAACCTATATCT
GGCAACGGACGATCCAAGTAAACAAGCCTCTTTAAAGGAAATTCAGTTTCACACAGGTCTTAACACCAACGCAATTGTTG
TTGAAACTGATAAGTTATCTGCATTAATCGATAAATTACTGACAGCTAAGGAAAGTGAAGGGTTATCAGAATACGTTGAG
GATGCTGGTGATATTGATGGCCTCGAGATAAGTACGGATGAAGAAGATCAGGATAGTGATGTATCATCAGTGACTGATGA
TGCACCTATCGTGAAATTCATTAATAAAATACTGTTGGACGCAATTAAGCAAGGAACATCCGACATACACTTTGAGCCTT
ATGAAAAAGAATATCGAATTAGGTACAGACAAGATGGTATATTGCATGAAGTCGCAGCACCTCCAGTTAATTTATCAGCA
CGAATTACAGCACGGATCAAAGTAATGTCAAATTTGGATATCTCCGAAAGAAGAGTACCTCAAGATGGTGGCTTTAAAAT
GAAAATATCCAAAAATCGAGCGATTGATTTTAGGGTAAGTACCTGTCCAACCTATACCGGTGAAAAGGTGGTCATGAGAA
TCCTAGACCCAAGTACTGCTAAATTAGGAGTAGATGCACTTGGTTTTGCACAATATCAAAAAGAAGCATTTATTAGAGCT
ATTGAGAAACCACAAGGAATGATTCTTGTTACTGGTCCGACCGGTAGCGGTAAAACAGTAACTTTATACACTGCATTAAA
TATGTTAAACACAACTGAGGTGAATATCTCAACTGCAGAGGATCCTGTTGAGATCAAAGTTCCAGGGATTAACCAAGTCA
ACATCAATACGAAGGCTGGTTTAACATTCTCTGGCGCTCTTAGGTCCTTTCTTCGGCAAGATCCTGATATTATAATGGTT
GGGGAAATCAGAGATTTAGAAACTGCTGAAATTGCGGTAAAAGCAGCTCAGACTGGTCATTTGGTGCTCTCTACACTTCA
TACAAATAGTGCTGCAGAAACCCTTAATCGACTTGTTAATATGGGTATTCCAACATTTAACATAGCAAGTTCTGTTACTC
TGATTATTGCTCAGCGATTAGCACGTAGACTATGTGAACAATGCAAAGTACTGCGTGATGATGTCAGTGATAAAGGTTTA
TTAGAGTTAGGATTTAAAGAAGAAGATCTGGTTGGCCTCAAACTCTATAAAGCGGTTGGATGTAATCAATGCACTAATGG
ATTCAGAGGTAGAGTAGGTTTGTATGAAGTGCTAACCATGAACAAGGATTTGGGACAAATGATAATGAATGGAGCAAATT
CACTTGAAATCCTTAAGTTTGCTCAATCACAAGGTATGCATACTATATTTGAATCTGGTTTAGAAAAAGTTAAGGAAGGA
GTTACTACCATGGAGGAAGTCAATAGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W1A169

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

83.798

100

0.839
  pilB Acinetobacter baumannii D1279779

51.59

98.778

0.51
  pilB Acinetobacter baylyi ADP1

49.829

100

0.508
  pilB Vibrio cholerae strain A1552

51.04

92.321

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

47.842

97.033

0.464
  pilB Vibrio campbellii strain DS40M4

47.133

97.382

0.459
  pilF Neisseria gonorrhoeae MS11

45.76

98.778

0.452
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.583

92.147

0.365

Multiple sequence alignment