Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   CKV83_RS04850 Genome accession   NZ_LT906439
Coordinates   1045429..1046268 (-) Length   279 a.a.
NCBI ID   WP_018372876.1    Uniprot ID   A0A239SSK8
Organism   Streptococcus merionis strain NCTC13788     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1040429..1051268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV83_RS04830 (SAMEA4412692_00969) - 1041998..1042528 (+) 531 WP_018372872.1 hypothetical protein -
  CKV83_RS04835 (SAMEA4412692_00970) - 1042528..1043016 (+) 489 WP_018372873.1 hypothetical protein -
  CKV83_RS04840 (SAMEA4412692_00971) - 1043898..1044089 (+) 192 WP_018372874.1 helix-turn-helix transcriptional regulator -
  CKV83_RS04845 (SAMEA4412692_00972) - 1044501..1045346 (-) 846 WP_018372875.1 class I SAM-dependent methyltransferase -
  CKV83_RS04850 (SAMEA4412692_00973) dprA 1045429..1046268 (-) 840 WP_018372876.1 DNA-processing protein DprA Machinery gene
  CKV83_RS04855 (SAMEA4412692_00974) - 1046431..1047642 (-) 1212 WP_018372877.1 nucleotide sugar dehydrogenase -
  CKV83_RS04860 (SAMEA4412692_00975) - 1047663..1048850 (-) 1188 WP_018372878.1 glycosyltransferase family 4 protein -
  CKV83_RS04865 (SAMEA4412692_00976) - 1048966..1050393 (-) 1428 WP_018372879.1 lipopolysaccharide biosynthesis protein -
  CKV83_RS04870 (SAMEA4412692_00977) - 1050393..1051259 (-) 867 WP_018372880.1 glycosyltransferase -

Sequence

Protein


Download         Length: 279 a.a.        Molecular weight: 30984.47 Da        Isoelectric Point: 6.6031

>NTDB_id=1147132 CKV83_RS04850 WP_018372876.1 1045429..1046268(-) (dprA) [Streptococcus merionis strain NCTC13788]
MKAYDIFKWKQAGLTNLQINRILDYEQKAGKKLSDRDIVIVSEVKNPAVILEHYRNLDEAQCKERFDCFPALSILDEDYP
LALREIYNPPTLLFYQGNLELLHQPKLAIVGSRNASKTGAGAVQKIVKELDNHFVIVSGLARGIDTAAHMAVLKNGGQTI
AVIGTGLDVFYPKENRELQGYMSRNQLVLTEYEPSQQPLKFHFPERNRIIAGLASGVVVVEAKMRSGSLITCERAIEEGR
DVFAVPGSILDGQSDGCHHLIREGAKCTNSGQDILSEYA

Nucleotide


Download         Length: 840 bp        

>NTDB_id=1147132 CKV83_RS04850 WP_018372876.1 1045429..1046268(-) (dprA) [Streptococcus merionis strain NCTC13788]
ATGAAAGCATATGATATCTTTAAGTGGAAGCAGGCAGGGCTGACTAATCTGCAAATCAATCGAATTTTGGACTATGAACA
GAAAGCGGGTAAAAAGCTGTCAGATCGCGATATAGTCATCGTTTCAGAGGTGAAAAATCCTGCGGTTATTCTGGAGCATT
ATCGCAATCTCGATGAGGCTCAGTGTAAGGAGAGGTTTGACTGTTTTCCTGCCTTGTCTATCTTGGATGAGGATTATCCT
TTAGCTTTGAGAGAAATCTATAATCCGCCAACTTTGCTTTTCTACCAAGGGAATTTGGAGTTGTTACACCAGCCTAAGTT
AGCTATCGTTGGGAGTCGTAATGCTAGTAAGACTGGGGCGGGTGCAGTTCAGAAAATCGTCAAAGAGTTGGATAATCATT
TTGTCATCGTCAGTGGCTTGGCGCGTGGAATCGATACTGCAGCCCACATGGCTGTGCTTAAAAATGGTGGGCAGACTATT
GCGGTTATTGGTACGGGATTGGATGTCTTTTATCCCAAGGAAAATCGGGAACTGCAGGGCTATATGAGCCGAAATCAGCT
GGTCTTGACAGAGTATGAACCGAGTCAACAGCCACTCAAGTTCCATTTTCCAGAGCGCAATCGCATTATCGCGGGATTGG
CTAGTGGTGTGGTTGTCGTTGAGGCCAAGATGCGTTCAGGTAGTCTCATTACCTGTGAGCGAGCTATAGAAGAAGGACGC
GATGTCTTTGCAGTCCCAGGCAGCATCTTAGATGGTCAGTCCGATGGCTGCCATCATCTCATTCGTGAGGGAGCCAAATG
TACCAATTCGGGTCAGGATATTTTATCCGAGTATGCTTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-60)

DNA recombination-mediator protein A (DNA_processg_A)

(72-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A239SSK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Streptococcus mutans UA159

66.547

99.642

0.663
  dprA/cilB/dalA Streptococcus mitis SK321

63.636

98.566

0.627
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

63.273

98.566

0.624
  dprA/cilB/dalA Streptococcus pneumoniae D39

63.273

98.566

0.624
  dprA/cilB/dalA Streptococcus pneumoniae R6

63.273

98.566

0.624
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

63.273

98.566

0.624
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

62.909

98.566

0.62
  dprA Lactococcus lactis subsp. cremoris KW2

58.909

98.566

0.581
  dprA Legionella pneumophila strain ERS1305867

43.496

88.172

0.384
  dprA Acinetobacter baylyi ADP1

39.925

96.057

0.384
  dprA Bacillus subtilis subsp. subtilis str. 168

42.623

87.455

0.373
  dprA Haemophilus influenzae Rd KW20

38.636

94.624

0.366

Multiple sequence alignment