Detailed information NTG1147123

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: CKV83_RS02440
• Genome accession: NZ_LT906439
• Coordinates: 534862..535524 (+)
• Length: 220 a.a.
• NCBI ID: WP_018373726.1
• Uniprot ID: -
• Organism: Streptococcus merionis strain NCTC13788
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 529862..540524

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKV83_RS02415 (SAMEA4412692_00487)	-	530321..530899 (-)	579	WP_018373731.1	xanthine phosphoribosyltransferase	-
CKV83_RS02420 (SAMEA4412692_00488)	-	531590..532906 (-)	1317	WP_018373730.1	nucleobase:cation symporter-2 family protein	-
CKV83_RS02425 (SAMEA4412692_00489)	-	533011..533271 (-)	261	WP_018373729.1	GIY-YIG nuclease family protein	-
CKV83_RS02430 (SAMEA4412692_00490)	-	533261..534007 (-)	747	WP_018373728.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
CKV83_RS02435 (SAMEA4412692_00491)	-	534073..534819 (+)	747	WP_018373727.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
CKV83_RS02440 (SAMEA4412692_00492)	comEA/celA/cilE	534862..535524 (+)	663	WP_018373726.1	helix-hairpin-helix domain-containing protein	Machinery gene
CKV83_RS02445 (SAMEA4412692_00493)	comEC/celB	535508..537745 (+)	2238	WP_018373725.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CKV83_RS02450 (SAMEA4412692_00494)	-	538007..538879 (+)	873	WP_026216957.1	helix-turn-helix domain-containing protein	-
CKV83_RS02455 (SAMEA4412692_00495)	tnpA	538999..539472 (+)	474	WP_018373723.1	IS200/IS605 family transposase	-
CKV83_RS11385	-	539717..539854 (+)	138	WP_018373722.1	hypothetical protein	-
CKV83_RS02460 (SAMEA4412692_00496)	-	540130..540426 (+)	297	WP_018373721.1	lactococcin 972 family bacteriocin	-

Sequence

Protein

• Length: 220 a.a.
• Molecular weight: 22999.68 Da
• Isoelectric Point: 4.1419

>NTDB_id=1147123 CKV83_RS02440 WP_018373726.1 534862..535524(+) (comEA/celA/cilE) [Streptococcus merionis strain NCTC13788]
MLDKMLEIVKENKITAALAGLGLMIGAAFVLFNQPTQAEQVPSVTALASQVNSTSDSSAEETPLPSKEEEESKTIMVDVK
GAVQKPGLYKLEAGSRLADAVAEAGGLSEEADSKSINLAQKLSDEAVVYVAKQGEEVSVVQSSAATGTSSTSQSGNDLVN
INTATLAELQTISGIGAKRAQDIIDYREANGGFKTIDDLNNVSGIGDKTMENLRAYVTVD

Nucleotide

• Length: 663 bp

>NTDB_id=1147123 CKV83_RS02440 WP_018373726.1 534862..535524(+) (comEA/celA/cilE) [Streptococcus merionis strain NCTC13788]
ATGTTGGATAAGATGTTAGAAATTGTCAAGGAAAATAAGATTACAGCAGCTCTAGCAGGTCTAGGGCTGATGATTGGAGC
AGCTTTTGTCTTGTTTAATCAACCAACTCAAGCTGAGCAGGTACCCTCAGTTACTGCCTTAGCAAGCCAAGTCAATAGCA
CAAGCGACTCATCTGCTGAGGAAACACCATTGCCATCAAAAGAGGAAGAAGAATCCAAGACTATCATGGTTGATGTCAAA
GGGGCAGTCCAAAAGCCCGGCCTATACAAGCTGGAAGCGGGTAGTCGTCTAGCGGATGCTGTGGCAGAAGCAGGAGGTTT
GAGCGAGGAGGCAGACAGTAAATCCATTAATCTGGCACAGAAATTGAGCGACGAAGCAGTCGTCTACGTAGCCAAGCAGG
GCGAGGAAGTCAGCGTCGTCCAAAGCTCAGCAGCAACAGGAACTTCAAGCACTTCTCAATCTGGTAACGACCTAGTCAAT
ATCAATACTGCCACCCTAGCTGAACTCCAGACCATCTCAGGTATCGGTGCCAAGCGCGCTCAGGACATCATCGATTACCG
CGAAGCCAATGGCGGCTTCAAAACCATTGACGATCTCAACAATGTCTCAGGTATCGGCGACAAAACCATGGAGAATTTGA
GAGCCTATGTTACGGTGGATTAG

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-130)

• Helix-hairpin-helix motif (HHH_3) (157-218)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus mitis NCTC 12261	48.402	99.545	0.482
comEA	Streptococcus thermophilus LMD-9	45.852	100	0.477
comEA/celA/cilE	Streptococcus pneumoniae Rx1	47.945	99.545	0.477
comEA/celA/cilE	Streptococcus pneumoniae D39	47.945	99.545	0.477
comEA/celA/cilE	Streptococcus pneumoniae R6	47.945	99.545	0.477
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	47.945	99.545	0.477
comEA/celA/cilE	Streptococcus mitis SK321	47.945	99.545	0.477
comEA	Lactococcus lactis subsp. cremoris KW2	43.049	100	0.436