Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC/celB   Type   Machinery gene
Locus tag   CKV83_RS02445 Genome accession   NZ_LT906439
Coordinates   535508..537745 (+) Length   745 a.a.
NCBI ID   WP_018373725.1    Uniprot ID   A0A239SQV7
Organism   Streptococcus merionis strain NCTC13788     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 530508..542745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV83_RS02420 (SAMEA4412692_00488) - 531590..532906 (-) 1317 WP_018373730.1 nucleobase:cation symporter-2 family protein -
  CKV83_RS02425 (SAMEA4412692_00489) - 533011..533271 (-) 261 WP_018373729.1 GIY-YIG nuclease family protein -
  CKV83_RS02430 (SAMEA4412692_00490) - 533261..534007 (-) 747 WP_018373728.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  CKV83_RS02435 (SAMEA4412692_00491) - 534073..534819 (+) 747 WP_018373727.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  CKV83_RS02440 (SAMEA4412692_00492) comEA/celA/cilE 534862..535524 (+) 663 WP_018373726.1 helix-hairpin-helix domain-containing protein Machinery gene
  CKV83_RS02445 (SAMEA4412692_00493) comEC/celB 535508..537745 (+) 2238 WP_018373725.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CKV83_RS02450 (SAMEA4412692_00494) - 538007..538879 (+) 873 WP_026216957.1 helix-turn-helix domain-containing protein -
  CKV83_RS02455 (SAMEA4412692_00495) tnpA 538999..539472 (+) 474 WP_018373723.1 IS200/IS605 family transposase -
  CKV83_RS11385 - 539717..539854 (+) 138 WP_018373722.1 hypothetical protein -
  CKV83_RS02460 (SAMEA4412692_00496) - 540130..540426 (+) 297 WP_018373721.1 lactococcin 972 family bacteriocin -
  CKV83_RS02465 - 540528..542546 (+) 2019 WP_018373720.1 bacteriocin-associated integral membrane family protein -

Sequence

Protein


Download         Length: 745 a.a.        Molecular weight: 84090.90 Da        Isoelectric Point: 8.9437

>NTDB_id=1147124 CKV83_RS02445 WP_018373725.1 535508..537745(+) (comEC/celB) [Streptococcus merionis strain NCTC13788]
MLRWIRTTALPPIQLALMLIVLFYTLRFPSPITLFLLGFLCWRLFWKHSRAVIQASLMILVPFGFFFVAQRYLAAQANHS
APESISQLQMIPDSIAVNGDRLSFRAKEAGQTYQFFYKLKTQEEQAFFQKLSQPVRLTVEAEVSEAESQRNFNGFDYQAY
LETQGIYKTVQVKTISEIVPVHSWNVFDLLSKWRRSALVYIHQQFPNPMRHYMSGLLFGHLDKDFDQMGDLYSGLGIIHL
FALSGMQVGFFVDKFRWLLLRLGLTQETVDWLQLPFSLFYAGMTGFSASVIRSLIQKNLANQGFSGLNNLGLTVLISFVL
MPNFLLTAGGVLSFGYAFILTMQDFEHLTGIKRALSEAFALSLAILPVLIWYFATFQPLSILLTFAFSLLFDVLFLPLLS
LVFLLSPLWKIAELNSLFVGLEGIIRWVAGLASRPLVFGKPSLLVLVFLLISLGLLYDFWQHKKARVGLGVLIIGLFLIT
KCPLTNEVTVVDIGQGDSIFLRDMKGRNVLIDVGGKVSFSGKEAWQQAQSDSNAERTLIPYLKSRGVSKIDTLVLTHTDT
DHIGDLLPVAKNFDIGQIWVSSGSLTKADFVKTLESLGVPVHVTKVGDQIPICDSYLEVLYPREIGDGGNNDSIVLYGQL
LQTRFLFTGDLEHGELDLIKAYPNLRVDVLKAGHHGSKGSSYPEFLDHIKPQIGLFSAGKNNRYKHPHPETVARFDERNI
TTYRTDQQGAIRFHGWKTWQVETVR

Nucleotide


Download         Length: 2238 bp        

>NTDB_id=1147124 CKV83_RS02445 WP_018373725.1 535508..537745(+) (comEC/celB) [Streptococcus merionis strain NCTC13788]
ATGTTACGGTGGATTAGAACGACAGCTCTCCCACCCATCCAACTAGCCTTGATGCTTATTGTACTTTTCTACACCCTGCG
CTTTCCCAGTCCTATCACCTTGTTTTTGCTGGGCTTTTTATGCTGGCGGCTTTTTTGGAAACATAGCAGAGCAGTGATTC
AGGCTAGCTTGATGATTTTAGTTCCCTTTGGCTTCTTTTTTGTTGCTCAGCGCTATCTGGCCGCTCAGGCCAATCATTCT
GCGCCTGAAAGCATTAGCCAATTGCAGATGATCCCAGACAGCATCGCTGTCAATGGGGATCGTCTATCGTTTAGAGCTAA
GGAAGCTGGTCAGACCTATCAGTTTTTTTACAAACTCAAAACGCAAGAAGAACAGGCCTTCTTTCAAAAGCTATCTCAGC
CAGTCCGCTTGACGGTAGAAGCAGAAGTGTCAGAAGCTGAGAGTCAGCGCAATTTCAACGGTTTTGACTACCAAGCCTAT
CTGGAAACCCAAGGGATTTACAAGACGGTGCAGGTCAAGACTATTTCTGAGATTGTACCAGTTCACTCTTGGAATGTCTT
TGACCTTCTTTCCAAGTGGCGTCGCAGTGCTCTAGTCTATATTCATCAGCAGTTTCCCAATCCCATGCGCCATTACATGT
CAGGCCTGCTTTTTGGCCATTTGGATAAGGATTTTGATCAGATGGGAGATCTTTATTCCGGTCTTGGCATTATTCACCTC
TTTGCCCTGTCTGGCATGCAGGTTGGCTTCTTTGTGGATAAGTTTCGCTGGCTATTGCTCCGTTTGGGTCTAACTCAGGA
AACGGTGGATTGGCTGCAACTCCCTTTTTCCTTGTTTTATGCTGGTATGACTGGTTTTTCGGCCTCGGTCATTCGTAGTC
TGATTCAGAAAAACTTGGCTAATCAAGGGTTTTCAGGACTTAATAACCTTGGCTTAACCGTCCTTATCAGCTTTGTCCTC
ATGCCTAATTTTTTACTAACAGCGGGTGGGGTTCTCAGTTTTGGCTATGCCTTTATCCTGACCATGCAGGACTTTGAGCA
TCTGACAGGCATCAAGCGCGCACTATCTGAGGCTTTTGCCCTCTCACTTGCTATCCTCCCCGTCCTCATCTGGTACTTCG
CTACCTTCCAGCCCCTCTCAATCCTACTAACCTTTGCCTTTTCTCTGCTCTTTGATGTCCTCTTCTTACCTTTGCTGAGC
TTGGTCTTTTTGCTTTCCCCTTTATGGAAAATAGCAGAGCTCAATAGTTTATTTGTCGGTTTGGAGGGCATTATTCGGTG
GGTAGCAGGTTTAGCTAGCCGACCGCTCGTCTTTGGCAAGCCCAGTCTACTGGTTTTGGTTTTCTTATTGATTAGCCTTG
GTCTGCTCTATGATTTTTGGCAGCATAAAAAGGCCAGAGTCGGCCTTGGAGTTCTGATTATCGGACTTTTTCTCATCACT
AAGTGCCCGCTGACCAATGAAGTGACTGTGGTTGACATTGGCCAAGGCGACAGCATTTTCCTGAGAGATATGAAAGGTCG
CAATGTGCTTATTGATGTTGGCGGTAAGGTAAGCTTCAGTGGCAAGGAAGCATGGCAGCAAGCTCAGTCTGACAGCAATG
CAGAGCGTACGCTGATTCCTTATCTAAAAAGCCGCGGCGTAAGCAAAATCGATACCCTTGTTCTGACCCATACCGACACG
GACCATATCGGTGATTTGCTGCCGGTAGCCAAAAACTTTGACATTGGTCAAATCTGGGTGTCATCAGGTAGCTTGACTAA
GGCAGACTTTGTCAAAACGTTAGAAAGTCTTGGTGTTCCTGTTCATGTGACCAAGGTGGGCGATCAGATTCCTATCTGCG
ATAGCTACCTAGAGGTGCTCTACCCTAGAGAAATTGGTGATGGTGGCAATAATGATTCGATAGTCCTTTATGGGCAACTG
CTCCAGACCAGATTTCTCTTTACAGGTGACTTGGAGCATGGAGAGCTAGATTTGATTAAGGCCTATCCTAATCTCAGGGT
TGATGTCCTCAAAGCTGGACACCACGGCAGTAAAGGGTCTTCGTATCCAGAATTTCTGGATCATATCAAGCCTCAGATTG
GCCTTTTCTCTGCTGGAAAAAATAACCGCTACAAACATCCGCACCCAGAAACAGTCGCGCGATTCGATGAGAGAAACATC
ACCACCTACCGCACAGACCAACAAGGCGCCATCCGCTTTCACGGTTGGAAAACCTGGCAAGTAGAGACGGTGAGATGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(32-177)

Metallo-beta-lactamase superfamily (Lactamase_B)

(494-689)

Competence protein (Competence)

(216-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A239SQV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC/celB Streptococcus mitis SK321

54.993

100

0.554
  comEC/celB Streptococcus mitis NCTC 12261

54.667

100

0.55
  comEC/celB Streptococcus pneumoniae TIGR4

53.662

100

0.541
  comEC/celB Streptococcus pneumoniae Rx1

53.262

100

0.537
  comEC/celB Streptococcus pneumoniae D39

53.262

100

0.537
  comEC/celB Streptococcus pneumoniae R6

53.262

100

0.537
  comEC Lactococcus lactis subsp. cremoris KW2

48.309

99.195

0.479

Multiple sequence alignment