Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CKW10_RS22255 Genome accession   NZ_LT906435
Coordinates   5062583..5063755 (+) Length   390 a.a.
NCBI ID   WP_084104044.1    Uniprot ID   A0A239SW42
Organism   Pandoraea sputorum strain NCTC13161     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5057583..5068755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW10_RS22225 (SAMEA4530655_04485) cgtA 5057688..5058818 (-) 1131 WP_039393308.1 Obg family GTPase CgtA -
  CKW10_RS22230 (SAMEA4530655_04486) rpmA 5058968..5059228 (-) 261 WP_039393311.1 50S ribosomal protein L27 -
  CKW10_RS22235 (SAMEA4530655_04487) rplU 5059257..5059568 (-) 312 WP_039393314.1 50S ribosomal protein L21 -
  CKW10_RS22240 (SAMEA4530655_04488) - 5059896..5060825 (+) 930 WP_224786948.1 polyprenyl synthetase family protein -
  CKW10_RS22250 (SAMEA4530655_04491) - 5061149..5062441 (+) 1293 WP_039393320.1 HlyC/CorC family transporter -
  CKW10_RS22255 (SAMEA4530655_04492) pilF 5062583..5063755 (+) 1173 WP_084104044.1 GspE/PulE family protein Machinery gene
  CKW10_RS22260 (SAMEA4530655_04493) - 5063894..5065120 (+) 1227 WP_039393323.1 type II secretion system F family protein -
  CKW10_RS22265 (SAMEA4530655_04494) - 5065117..5065977 (+) 861 WP_052252325.1 A24 family peptidase -
  CKW10_RS22270 (SAMEA4530655_04495) coaE 5065974..5066588 (+) 615 WP_039393325.1 dephospho-CoA kinase -
  CKW10_RS22275 (SAMEA4530655_04496) zapD 5066764..5067519 (+) 756 WP_039393327.1 cell division protein ZapD -
  CKW10_RS22280 (SAMEA4530655_04497) yacG 5067524..5067718 (+) 195 WP_039393329.1 DNA gyrase inhibitor YacG -
  CKW10_RS22285 (SAMEA4530655_04498) - 5067855..5068289 (-) 435 WP_039401447.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 41994.23 Da        Isoelectric Point: 7.4150

>NTDB_id=1146965 CKW10_RS22255 WP_084104044.1 5062583..5063755(+) (pilF) [Pandoraea sputorum strain NCTC13161]
MLRPTIASDDALPSTGTPASTTPLQRLEQILSEAIRAGASDIHFEPMAQRFRVRLRVDGRLQEHGDVPLTARDMLVSRLK
VLANLDIAQKRLPQDGRMVWREAGEPVECRVSALPTLHGEKLVVRLLDGAKVPLSLEGLGYSPKQYLALTQAIARPHGLI
LLTGPTGSGKTVSLYSCLRRLNDASRNIVTIEDPVEIRLAGITQVNLNERAGLDFATALRAFLRQDPDVLVVGEIRDAQT
AEIAIQAAQTGHLVFATVHANDAPSTLARLFDLGVAPFNLSSTLLLVSAQRLLRRRCPAGCEPSRTTTTETGLADTCMRC
HGTGFAGRIGAFQVMPISATQAINIAQARSPHELAAQACREGVMTLREAGLWHVDAGAVAQEDVDATLPA

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=1146965 CKW10_RS22255 WP_084104044.1 5062583..5063755(+) (pilF) [Pandoraea sputorum strain NCTC13161]
ATGCTGCGTCCAACCATCGCCTCCGACGACGCTCTGCCGTCCACCGGCACCCCGGCGTCCACCACACCTCTGCAACGCCT
TGAGCAAATACTGAGCGAAGCGATTCGTGCGGGCGCTTCCGATATCCATTTCGAACCGATGGCGCAGCGCTTTCGGGTCC
GTCTGCGTGTCGACGGTCGCCTGCAGGAGCATGGCGACGTTCCCCTGACAGCGCGCGACATGCTGGTCTCGCGTCTGAAG
GTGCTTGCCAATCTCGACATCGCACAAAAGCGCTTGCCTCAGGACGGACGCATGGTGTGGCGCGAAGCTGGTGAACCGGT
CGAGTGTCGCGTGAGTGCGCTCCCCACCCTGCACGGCGAGAAACTCGTGGTGCGCCTGCTCGATGGCGCGAAAGTCCCTC
TCTCGCTTGAAGGTCTCGGCTATTCGCCGAAGCAATACCTCGCACTCACGCAAGCGATTGCCCGGCCACATGGCCTGATC
TTGCTGACGGGCCCGACGGGCAGTGGCAAGACCGTCTCGCTCTATAGTTGCCTGCGACGTCTGAACGACGCGTCGCGCAA
CATCGTCACCATCGAGGACCCGGTGGAAATTCGCTTGGCCGGTATCACGCAGGTCAATCTCAACGAACGCGCTGGCCTCG
ACTTTGCAACGGCTTTACGTGCGTTTCTCCGTCAGGATCCAGATGTCCTCGTCGTGGGCGAAATCCGCGATGCGCAGACC
GCCGAAATCGCCATACAGGCCGCGCAGACCGGTCACCTTGTGTTCGCCACCGTTCACGCCAACGACGCGCCGAGCACGCT
CGCGCGGCTCTTCGATCTCGGTGTCGCCCCGTTCAATCTGTCGTCGACGTTACTGCTGGTCAGCGCACAGCGTCTGCTTA
GGCGACGATGCCCGGCCGGGTGCGAGCCGTCCCGCACGACGACGACTGAAACAGGCCTCGCTGACACCTGCATGCGGTGT
CACGGCACCGGTTTTGCCGGACGCATCGGCGCGTTTCAGGTGATGCCGATCAGCGCCACGCAGGCTATCAACATTGCACA
GGCGCGCAGTCCGCACGAGCTGGCGGCGCAGGCATGCCGCGAAGGCGTCATGACGCTGCGAGAAGCCGGGTTATGGCATG
TCGATGCAGGGGCTGTCGCTCAGGAGGATGTCGATGCGACGCTGCCCGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(29-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A239SW42

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

48.462

100

0.485
  pilB Vibrio cholerae strain A1552

47.409

98.974

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.194

97.692

0.451
  pilB Acinetobacter baumannii D1279779

43.75

100

0.449
  pilB Vibrio campbellii strain DS40M4

47.297

94.872

0.449
  pilB Acinetobacter baylyi ADP1

45.789

97.436

0.446
  pilB Legionella pneumophila strain ERS1305867

42.786

100

0.441
  pilF Thermus thermophilus HB27

41.582

100

0.418
  pilB/pilB1 Synechocystis sp. PCC 6803

39.853

100

0.418
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.379

96.667

0.4
  pilB Haemophilus influenzae 86-028NP

42.149

93.077

0.392
  pilB Haemophilus influenzae Rd KW20

46.452

79.487

0.369
  pilB Glaesserella parasuis strain SC1401

38.919

94.872

0.369

Multiple sequence alignment