Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CFBP1590_RS04970 Genome accession   NZ_LT855380
Coordinates   1027898..1029598 (-) Length   566 a.a.
NCBI ID   WP_029243836.1    Uniprot ID   A0A1Y6JJZ8
Organism   Pseudomonas viridiflava strain CFBP 1590 isolate E12-5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1022898..1034598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFBP1590_RS04940 (CFBP1590__1041) - 1023394..1024023 (-) 630 WP_004884092.1 DUF1780 domain-containing protein -
  CFBP1590_RS04945 (CFBP1590__1042) - 1024148..1024609 (+) 462 WP_057409622.1 hypothetical protein -
  CFBP1590_RS04950 (CFBP1590__1043) yacG 1024978..1025187 (-) 210 WP_088234576.1 DNA gyrase inhibitor YacG -
  CFBP1590_RS04955 (CFBP1590__1044) coaE 1025184..1025807 (-) 624 WP_025993968.1 dephospho-CoA kinase -
  CFBP1590_RS04960 (CFBP1590__1045) pilD 1025804..1026676 (-) 873 WP_088234577.1 A24 family peptidase Machinery gene
  CFBP1590_RS04965 (CFBP1590__1046) pilC 1026678..1027895 (-) 1218 WP_088234578.1 type II secretion system F family protein Machinery gene
  CFBP1590_RS04970 (CFBP1590__1047) pilB 1027898..1029598 (-) 1701 WP_029243836.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CFBP1590_RS04975 (CFBP1590__1048) pilA2 1029823..1030260 (+) 438 WP_025993964.1 pilin Machinery gene
  CFBP1590_RS04980 (CFBP1590__1049) - 1030783..1031505 (-) 723 Protein_962 IS66 family transposase -
  CFBP1590_RS04985 (CFBP1590__1050) - 1031550..1033061 (-) 1512 WP_088234579.1 IS66 family transposase -
  CFBP1590_RS04990 (CFBP1590__1051) tnpB 1033080..1033439 (-) 360 WP_004883347.1 IS66 family insertion sequence element accessory protein TnpB -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62585.77 Da        Isoelectric Point: 6.2562

>NTDB_id=1146528 CFBP1590_RS04970 WP_029243836.1 1027898..1029598(-) (pilB) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
MSDVVVLTGLAKQLVVAELLSEKAAQQAFQQARRDKISLISYLVQNKLVKSLTLAEMASEQFGVPFMDISSLDKESQPKG
LVSEKLARQHHALPLWRRGNRLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTAAIDKFFDSDSGLGSMADIDLEL
DIEPGEGVKETSITSRDDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKVFRVRFRTDGILHEAAKPPINLASRIAARL
KVMASLDISERRKPQDGRVKLRVSKTKSIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKELYLEALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVNINISTAEDPVEINLEGVNQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKEVDVPHETLIKEGFPE
SKVGTFTLYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1146528 CFBP1590_RS04970 WP_029243836.1 1027898..1029598(-) (pilB) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
ATGAGTGATGTCGTTGTTCTTACCGGCCTGGCCAAGCAATTGGTCGTCGCCGAGCTTCTTTCCGAAAAAGCCGCTCAACA
GGCTTTCCAGCAGGCGCGACGCGACAAGATTTCGCTGATCAGCTATTTGGTTCAGAACAAACTGGTCAAGAGTCTCACGT
TGGCCGAAATGGCGTCCGAGCAGTTCGGCGTGCCATTCATGGACATATCAAGCCTCGATAAAGAAAGCCAGCCCAAGGGG
CTGGTAAGCGAAAAGCTCGCACGCCAACACCACGCCCTTCCCCTGTGGCGTCGGGGCAACAGACTGTTTATTGGCATCTC
GGACCCGACCAATCATCAGGCGGTGACCGACATCCAGTTCAGTACTGGTTTGAATACTGAAGCGATTCTGGTCGAAGACG
ACAAACTCACTGCAGCCATTGACAAGTTTTTTGACAGCGATAGCGGGCTCGGGTCCATGGCCGATATCGACCTGGAACTC
GATATCGAGCCGGGCGAAGGCGTCAAGGAAACATCTATTACCAGCCGCGACGACGCCGATGACGCTCCGGTAGTGCGTTT
CGTCAACAAAATGCTGCTGGATGCCATTCGCCTGGGCTCGTCCGATCTGCACTTCGAACCTTATGAAAAGGTGTTTCGCG
TGCGCTTTCGTACGGACGGGATTCTGCACGAGGCGGCAAAACCTCCTATCAATCTGGCCAGTCGTATCGCGGCGCGCCTG
AAGGTCATGGCCAGCCTGGACATCTCGGAACGGCGCAAGCCTCAGGACGGCCGGGTCAAGTTGCGTGTTTCCAAAACCAA
ATCCATCGACTTCCGGATGAACACGCTGCCGACGCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGATCCGACCAGTG
CACAGATGGGTATTGATGCGCTGGGCTACGAGCCGGATCAGAAAGAGCTGTATCTGGAAGCGCTCAAGCAGCCGCAGGGC
ATGATTCTGGTGACAGGCCCGACCGGCTCGGGCAAAACCGTTTCGTTGTACACAGGCCTTAATATTCTCAACACCGTGAA
TATCAATATTTCAACCGCCGAGGATCCGGTGGAGATCAACCTGGAAGGCGTCAATCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGTTGCGCGCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGTGAGATTCGCGACCTG
GAAACCGCCGAGATTGCGATCAAGGCCTCTCAGACCGGCCACATGGTGTTATCGACGCTGCACACCAACAGCGCAGCAGA
AACCCTGACACGCCTGCACCACATGGGTGTTGCGGCATTCAACATCGCCACGGCGATCAATCTGATCATTGCTCAGCGAC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAGGAAGTCGACGTCCCACACGAGACGCTGATCAAGGAAGGCTTCCCGGAG
TCGAAAGTCGGCACCTTCACACTGTACGGCCCGGTCGGATGCGAACACTGCAACGGCGGCTATCGAGGCCGAGTCGGGAT
TTACGAGGTCGTGAAAAAAACGCCGGAACTGGAACGCATCATCATGGAAGAAGGCAACTCGCTGGAGATTTCCAGGCAGA
TGCGCAAGGATGGTTTCAATGACCTGCGAACATCCGGCTTGTCGAAAGCCATGCAGGGCATTACCAGCCTGGAAGAAGTC
AATCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y6JJZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.406

99.293

0.56
  pilB Acinetobacter baylyi ADP1

56.228

99.293

0.558
  pilB Legionella pneumophila strain ERS1305867

54.561

100

0.549
  pilB Vibrio cholerae strain A1552

50.446

99.117

0.5
  pilB Vibrio campbellii strain DS40M4

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

49.186

97.703

0.481
  pilF Neisseria gonorrhoeae MS11

47.957

99.47

0.477
  pilF Thermus thermophilus HB27

38.732

100

0.389

Multiple sequence alignment