Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BQ6873_RS14955 Genome accession   NZ_LT671418
Coordinates   3146276..3148003 (-) Length   575 a.a.
NCBI ID   WP_076593359.1    Uniprot ID   -
Organism   Herminiimonas arsenitoxidans strain AS8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3141276..3153003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BQ6873_RS14925 - 3141959..3142378 (+) 420 WP_076593353.1 NUDIX domain-containing protein -
  BQ6873_RS14930 yacG 3142389..3142583 (-) 195 WP_076593354.1 DNA gyrase inhibitor YacG -
  BQ6873_RS14935 zapD 3142598..3143353 (-) 756 WP_076593355.1 cell division protein ZapD -
  BQ6873_RS14940 coaE 3143533..3144159 (-) 627 WP_076593356.1 dephospho-CoA kinase -
  BQ6873_RS14945 pilD 3144165..3145025 (-) 861 WP_157889230.1 A24 family peptidase Machinery gene
  BQ6873_RS14950 pilC 3145028..3146260 (-) 1233 WP_076593358.1 type II secretion system F family protein Machinery gene
  BQ6873_RS14955 pilB 3146276..3148003 (-) 1728 WP_076593359.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BQ6873_RS14960 ispB 3148217..3149206 (-) 990 WP_076593360.1 octaprenyl diphosphate synthase -
  BQ6873_RS14965 rplU 3149447..3149758 (+) 312 WP_041322652.1 50S ribosomal protein L21 -
  BQ6873_RS14970 rpmA 3149797..3150054 (+) 258 WP_076593361.1 50S ribosomal protein L27 -
  BQ6873_RS14975 cgtA 3150146..3151255 (+) 1110 WP_076593362.1 Obg family GTPase CgtA -
  BQ6873_RS14980 proB 3151343..3152461 (+) 1119 WP_076593363.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62626.20 Da        Isoelectric Point: 4.8558

>NTDB_id=1145960 BQ6873_RS14955 WP_076593359.1 3146276..3148003(-) (pilB) [Herminiimonas arsenitoxidans strain AS8]
MAAVLPSSASNNAMPGLARALLQAGRLTQQQADTISKQATTEKSPFIDMLLKSEVIDSGRLAAFCSETFGYPLLDLAAFN
SSLLPEKIIDAKLMQGQRVIALAKRGNKVSIAISDPTNSQALDQIKFQTELAVEPIIVDHNTLLKLIEKLGQSAEQDLNE
LIGDDLESIDFAEEDLAAPVDGATADIDDAPVVKFLQKILVDAINLGASDLHFEPFEKFYRIRFRVDGVLRDIAQPPLAI
KEKLASRIKVISKLDISEKRVPQDGRMKLVMSKTRSIDFRVSTLPTLFGEKIVMRILDGSQAQMGIEALGYDPDQKETLM
NAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDRAGLTFPVALKAFLRQDPDII
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPATLTRLMNMGVAPFNIASSVILITAQRLTRRLCSCKQTTVIPDDVL
LEAGFTEDDLDGTWLPYKPVGCERCSGSGYKGRLGIYQIMPISEEIENIILNHGTSLEIEAQAKKDGVRNLRESGLVKVK
QGLTSLEEILGCTNV

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1145960 BQ6873_RS14955 WP_076593359.1 3146276..3148003(-) (pilB) [Herminiimonas arsenitoxidans strain AS8]
ATGGCTGCAGTCCTCCCTAGTTCGGCATCAAATAATGCAATGCCAGGTCTCGCCCGTGCGCTCTTGCAGGCAGGCAGGCT
GACGCAACAGCAAGCCGACACCATTTCCAAACAGGCCACGACCGAAAAAAGCCCGTTTATCGACATGCTGCTGAAAAGCG
AAGTGATCGATTCAGGCAGACTCGCCGCTTTTTGTTCTGAAACATTTGGTTATCCATTACTGGATTTAGCGGCATTCAAC
AGCAGCCTGCTGCCAGAAAAAATCATTGATGCGAAATTGATGCAAGGTCAGCGCGTCATCGCCTTGGCCAAGCGCGGCAA
TAAAGTATCGATTGCCATTTCGGACCCGACCAATTCGCAAGCACTGGATCAAATCAAGTTTCAGACCGAATTGGCGGTTG
AACCCATCATCGTCGATCACAATACGCTGCTTAAGCTGATTGAAAAACTCGGGCAAAGTGCCGAGCAGGATTTGAATGAG
TTGATCGGTGACGATCTCGAAAGCATCGATTTTGCCGAAGAAGATCTCGCTGCACCTGTCGATGGCGCGACCGCCGATAT
CGACGATGCACCTGTCGTCAAATTCCTGCAAAAAATTCTGGTCGATGCGATCAATCTCGGCGCCTCTGATCTTCACTTCG
AACCTTTCGAAAAGTTCTATCGCATCCGCTTTCGCGTCGATGGTGTGCTGCGTGATATCGCGCAGCCACCGCTGGCGATC
AAGGAAAAACTGGCTTCGCGTATCAAGGTCATTTCGAAGCTAGATATTTCTGAGAAACGCGTACCGCAAGACGGCCGCAT
GAAGCTGGTGATGTCCAAGACGCGCTCTATCGATTTCCGCGTCAGCACCTTGCCCACCTTATTCGGCGAAAAGATCGTCA
TGCGTATTCTGGACGGCTCGCAGGCGCAAATGGGGATCGAAGCACTAGGTTACGACCCGGACCAGAAAGAAACGCTGATG
AATGCCATCCAGCGTCCGTACGGCATGGTGCTCGTCACCGGCCCGACCGGCTCCGGCAAAACCGTTTCGCTGTACACCTG
CCTGAACATTCTGAACAAACCCGGCATCAACATCTCCACCGCGGAAGACCCGGCCGAGATCAATTTGCCCGGCGTCAATC
AGGTCAACGTCAACGACCGCGCCGGCCTGACCTTCCCGGTCGCACTAAAAGCTTTCTTGCGACAAGATCCTGACATCATC
ATGGTCGGTGAGATCCGTGACCTGGAAACGGCAGATATCGCGATCAAGGCGGCGCAAACTGGTCACATGGTGTTCTCCAC
ACTGCACACCAACGATGCGCCGGCCACATTGACGCGTCTGATGAATATGGGCGTAGCACCGTTCAACATCGCGTCGTCCG
TCATTCTGATTACCGCGCAACGACTGACACGCCGCTTGTGCTCGTGCAAGCAAACCACCGTGATTCCGGACGATGTGTTG
CTAGAAGCCGGCTTTACTGAGGACGATCTGGACGGCACCTGGTTGCCATACAAACCTGTAGGTTGCGAACGTTGCAGCGG
CAGCGGCTATAAAGGTCGCTTGGGCATTTACCAAATCATGCCTATCAGCGAAGAAATTGAAAACATCATCTTGAACCACG
GCACTTCCCTGGAAATAGAAGCGCAGGCCAAGAAAGACGGCGTGCGTAACTTGCGCGAATCAGGCTTGGTAAAAGTCAAA
CAGGGATTGACCAGTCTGGAAGAAATTCTAGGCTGCACAAACGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.319

98.087

0.543
  pilB Acinetobacter baumannii D1279779

54.291

99.304

0.539
  pilF Neisseria gonorrhoeae MS11

54.448

97.739

0.532
  pilB Legionella pneumophila strain ERS1305867

49.556

97.913

0.485
  pilB Vibrio cholerae strain A1552

46.071

97.391

0.449
  pilB Vibrio campbellii strain DS40M4

45.374

97.739

0.443
  pilB Vibrio parahaemolyticus RIMD 2210633

45.115

97.913

0.442
  pilF Thermus thermophilus HB27

39.851

93.391

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.36

92.348

0.363

Multiple sequence alignment