Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BFP94_RS04780 Genome accession   NZ_LT604072
Coordinates   1158825..1160561 (+) Length   578 a.a.
NCBI ID   WP_003477096.1    Uniprot ID   A0A1C3TKN1
Organism   Xanthomonas translucens pv. translucens DSM 18974 isolate peng1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1153825..1165561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFP94_RS04760 (BN444_01938) coaE 1154499..1155119 (-) 621 WP_003477105.1 dephospho-CoA kinase -
  BFP94_RS04765 (BN444_01937) - 1155133..1155996 (-) 864 WP_003477103.1 A24 family peptidase -
  BFP94_RS04770 (BN444_01936) pilC 1156003..1157262 (-) 1260 WP_003477101.1 type II secretion system F family protein Machinery gene
  BFP94_RS19820 - 1157649..1158086 (+) 438 WP_038232341.1 pilin -
  BFP94_RS04775 (BN444_01934) - 1158332..1158709 (-) 378 WP_080603077.1 hypothetical protein -
  BFP94_RS04780 (BN444_01933) pilB 1158825..1160561 (+) 1737 WP_003477096.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BFP94_RS04785 (BN444_01932) - 1160571..1161188 (-) 618 WP_003477095.1 Fic family protein -
  BFP94_RS04790 (BN444_01931) - 1161185..1161367 (-) 183 WP_003477093.1 YhfG family protein -
  BFP94_RS04795 (BN444_01930) pilR 1161556..1162944 (-) 1389 WP_003477091.1 sigma-54 dependent transcriptional regulator Regulator
  BFP94_RS04800 (BN444_01929) - 1163019..1164632 (-) 1614 WP_003477089.1 ATP-binding protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62960.28 Da        Isoelectric Point: 6.1466

>NTDB_id=1145019 BFP94_RS04780 WP_003477096.1 1158825..1160561(+) (pilB) [Xanthomonas translucens pv. translucens DSM 18974 isolate peng1]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELLHKYQVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGRLEVGAGDEDMGSGVDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRVLDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1145019 BFP94_RS04780 WP_003477096.1 1158825..1160561(+) (pilB) [Xanthomonas translucens pv. translucens DSM 18974 isolate peng1]
ATGAACGCTGTGACATCCGCCAATCTTGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGTTGCACAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCATACCAATTTGACGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGCACCCTGGAACAGTGGCAGGCGAGCAACGATGCGATCGGCGATGCGCTGGGC
GACGACGACGAGGCCATGGGCAGGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGCGGCGTCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCGTCGGATA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGATGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCGGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTGTCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGTATCCTCAACGACGAGACCCGCAACATTTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAGATCCGTGACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACAGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCCCGACCCAGCTGCCG
CACAATGCGCTGCTGGCCGAGGGTTTCAGCGAGGCGGAAGTGGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGATCTGCGCCAGTCGGCGCTGCTC
AAGGCACGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3TKN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.93

98.27

0.54
  pilB Acinetobacter baumannii D1279779

53.873

98.27

0.529
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

49.047

99.827

0.49
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.466

97.924

0.455
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.421

90.311

0.365
  pilF Thermus thermophilus HB27

40.385

89.965

0.363

Multiple sequence alignment