Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NCTC9997_RS01995 Genome accession   NZ_LT575468
Coordinates   423811..425316 (-) Length   501 a.a.
NCBI ID   WP_064977169.1    Uniprot ID   -
Organism   Plesiomonas shigelloides strain NCTC10360     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418811..430316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCTC9997_RS01970 (BAY29_RS01965) coaE 418973..419581 (-) 609 WP_064977166.1 dephospho-CoA kinase -
  NCTC9997_RS01975 (BAY29_RS01970) pilD 419583..420419 (-) 837 WP_230405846.1 prepilin peptidase Machinery gene
  NCTC9997_RS01980 (BAY29_RS01975) - 420707..421747 (+) 1041 WP_010862605.1 GMP reductase -
  NCTC9997_RS01985 (BAY29_RS01980) - 421946..422500 (+) 555 WP_064977167.1 YbhB/YbcL family Raf kinase inhibitor-like protein -
  NCTC9997_RS01990 (BAY29_RS01985) - 422574..423809 (-) 1236 WP_064977168.1 type II secretion system F family protein -
  NCTC9997_RS01995 (BAY29_RS01990) pilB 423811..425316 (-) 1506 WP_064977169.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NCTC9997_RS15400 (SAMEA2665130_00407) - 425372..425830 (-) 459 WP_064978408.1 pilin -
  NCTC9997_RS02005 (BAY29_RS02000) nadC 426602..427528 (-) 927 WP_064977170.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NCTC9997_RS02010 (BAY29_RS02005) ampD 427978..428583 (+) 606 WP_064978409.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NCTC9997_RS02015 (BAY29_RS02010) ampE 428659..429501 (+) 843 WP_010862598.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55430.18 Da        Isoelectric Point: 5.9030

>NTDB_id=1143880 NCTC9997_RS01995 WP_064977169.1 423811..425316(-) (pilB) [Plesiomonas shigelloides strain NCTC10360]
MDKKNTINKYHHLFFLFIEYKALPLQEDDNTLYLSIADINNYKAADAFRFATGKNIELVQADKKELYARIEELTGQKVDD
IVTAADSETLGELDELDGDSRSTDTAEVADEQDEAPIVRFINQMLSRAVRVKASDIHFEPYEKTYRVRFRIDGVLQEITA
PPQKLAQRLAARLKIMSRLDIAERRVPQDGRIKLKLSEGTTIDLRVSTLPTLFGEKVVLRILDSSAARLDIEQLGFTARD
QQLYINALARPQGLILVTGPTGSGKTVTLYTGLSRLNTPERNISTAEDPVEINLSGVNQTQINTRAGLTFASALRAFLRQ
DPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLSNMGVPAYNIASSVNLIIAQRLARRLCPHCREAIT
LSYAEQQAREFTPLQPDGITIYRAHKEGCDHCSSGYKGRTGIYEMMPMSGQLTDAILRGAHAPELTAIACQQGMLTLRQA
GCAKVVEGITSLEEIERVTGV

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1143880 NCTC9997_RS01995 WP_064977169.1 423811..425316(-) (pilB) [Plesiomonas shigelloides strain NCTC10360]
ATGGATAAGAAAAACACGATAAACAAATATCATCACCTATTTTTCCTTTTTATCGAATATAAAGCCCTTCCCCTACAAGA
AGACGACAACACGCTCTATTTATCCATCGCCGATATTAACAACTACAAAGCCGCCGATGCTTTTCGCTTCGCCACCGGAA
AAAATATCGAACTGGTGCAAGCAGATAAAAAAGAGCTGTATGCCCGTATTGAAGAGCTGACCGGCCAAAAAGTCGATGAC
ATTGTTACTGCCGCCGACAGCGAAACCTTGGGCGAGCTTGATGAGCTCGACGGTGATAGCCGCAGCACCGACACCGCCGA
AGTAGCCGATGAGCAAGATGAAGCGCCCATTGTGCGTTTTATCAACCAAATGCTCAGCCGCGCCGTACGGGTAAAAGCTT
CCGACATTCACTTCGAACCCTACGAGAAAACCTATCGGGTCCGCTTTCGTATTGATGGCGTCTTGCAAGAAATTACTGCG
CCACCACAAAAGCTGGCCCAGCGCCTCGCCGCACGCCTAAAAATCATGAGTCGGCTGGATATCGCCGAGCGCCGCGTACC
ACAAGATGGGCGCATCAAGCTTAAACTGAGCGAAGGCACCACCATTGATTTACGGGTTTCCACCCTGCCCACTCTGTTTG
GCGAAAAGGTGGTACTGCGGATTCTCGACTCCTCTGCCGCCCGTTTGGATATCGAGCAACTGGGCTTTACTGCCCGTGAC
CAACAGCTGTACATCAATGCCCTAGCACGTCCGCAGGGGTTGATTCTGGTTACCGGCCCAACCGGAAGCGGTAAAACCGT
CACCTTGTATACTGGCTTATCACGCCTGAATACCCCTGAGCGCAATATCTCAACGGCGGAAGACCCGGTAGAAATCAACT
TAAGCGGCGTCAACCAAACCCAGATTAACACCCGTGCCGGCCTGACTTTCGCCAGTGCACTGCGCGCCTTTTTACGCCAA
GATCCTGACGTGGTGATGGTCGGGGAGATTCGCGATCTAGAAACCGCCGAAATTGCGGTAAAAGCAGCACAAACCGGCCA
CTTGGTACTCTCCACGCTGCACACCAACTCAGCCGCCGAAACCTTAACCCGCCTATCGAATATGGGCGTACCAGCTTACA
ACATTGCCTCCTCGGTGAATCTGATCATTGCTCAGCGGTTGGCGAGGCGTTTATGCCCGCATTGCCGTGAAGCCATCACC
TTGAGCTATGCCGAACAACAAGCCCGTGAGTTCACCCCGCTACAACCGGATGGGATCACCATTTACCGCGCCCACAAAGA
AGGCTGCGATCACTGCAGCAGTGGCTACAAAGGGCGTACCGGTATTTACGAAATGATGCCGATGTCCGGCCAGCTGACCG
ATGCCATTTTACGCGGCGCTCACGCCCCTGAGCTCACCGCTATAGCCTGCCAACAGGGCATGCTGACCTTGCGTCAAGCA
GGTTGTGCCAAAGTGGTTGAGGGGATCACCTCATTGGAAGAAATCGAGCGTGTAACAGGAGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-76)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

57.927

98.204

0.569
  pilB Vibrio parahaemolyticus RIMD 2210633

57.724

98.204

0.567
  pilB Vibrio cholerae strain A1552

57.613

97.006

0.559
  pilB Acinetobacter baylyi ADP1

54.082

97.804

0.529
  pilB Acinetobacter baumannii D1279779

52.323

98.802

0.517
  pilB Legionella pneumophila strain ERS1305867

51.521

98.403

0.507
  pilF Neisseria gonorrhoeae MS11

46.735

97.804

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

42.136

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

98.204

0.409
  pilB Haemophilus influenzae Rd KW20

40.947

97.006

0.397
  pilB Haemophilus influenzae 86-028NP

40.779

97.405

0.397
  pilF Thermus thermophilus HB27

40.994

96.407

0.395
  pilB Glaesserella parasuis strain SC1401

39.059

97.605

0.381

Multiple sequence alignment